We narrowed to 114 results for: CHL

...Snell KD, Seo JS, Chua N-H, Strano MS (2019) Chloroplast-selective gene delivery and expression in planta...

...hsdR17(rK- mK+)  XL10 Gold Tetracycline and Chloramphenicol High competency cloning and propagation of ...

.... G., Otto, P., Zimmerman, K., Vidugiris, G., Machleidt, T., Robers, M. B., Benink, H. A., Eggers, C. ...

...light-activated cation channel, from the archaebacteria Chlamydomonas reinhardtii. When exposed to blue light, this...

... guide you in gRNA design. We have been using Benchling, but there are many other gRNA design tools available...

...27966527. PubMed Central PMCID: PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform...

... widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that...

.... Usage Guide sequences can be designed using Benchling or using similar design tools and ordered as standard...

...References Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine...

...watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal, mitochondrial, peroxisomal...

...Balazsi Matthew Bennett Carolyn Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins Tom...

...kits are quick, easy, and don’t involve phenol-chloroform.  The default option is to use the kit that ...

...Burkholderia sp. Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus...

... of two plasmids: pCas9: carries Cas9 and chloramphenicol resistance pCRISPR: carries a spacer targeting...

...JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev ...

...on our blog . Protein Ligand Plasmids HaloTag Chloralkane (CA) pMH-Halo tag - Mammalian Gateway Destination...

...mL RNase A TE or water-saturated phenol-chloroform Chloroform 100% ethanol or isopropanol 90% ethanol ...: Perform phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction removes...Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the...volume of chloroform to the recovered aqueous DNA layer. Repeat steps 2-4. Note: Phenol-chloroform is a hazardous...aqueous DNA sample. Pro-Tip Water-saturated phenol-chloroform can be used if TE-saturated is not available....without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant after step 6 and...

...56 °C for 30 min. 25 mM chloroquine diphosphate Dissolve 0.129 g of chloroquine diphosphate salt into 10...slowly add hydrochloric acid until the solution clears. Check the pH of the solution Use hydrochloric acid ... Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear MW 25,000 Da Microcentrifuge....X 10 cm tissue culture dish, Corning 430167 Hydrochloric acid Sodium hydroxide 0.22 μm polyethersulfone... will be basic and will need adjustment with hydrochloric acid first. Pro-Tip The pH of this solution ...add 10 mL fresh DMEM complete containing 25 µM chloroquine diphosphate and incubate ~5 h For 10 mL of DMEM...DMEM complete, add 10 µL of 25 mM chloroquine diphosphate. Dilute 18.9 µg of DNA into 500 µL of Opti-Pro...

...are also included: using Isopropanol or Lithium Chloride. This protocol was adapted from (Link opens in...Water-saturated Phenol 2 M Sodium Acetate pH 4 Chloroform/Isoamyl alcohol (49:1) 75% Ethanol RNase-free...for precipitation step, Option A) 7.5 M Lithium Chloride (for precipitation step, Option B) Glycogen (Optional...phenol, mix thoroughly by inversion. Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) and then shake vigorously...(s). You can use either Isopropanol or Lithium Chloride for this step. Isopropanol (Option A): Add 1 volume...aqueous layer. Incubate at -20°C for 1 hour. Lithium Chloride (Option B): LiCl selectively precipitates RNA ...from the homogenized sample(s). Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) per 1 mL of TRIzol® used...

... add hydrochloric acid until the solution clears. Check the pH of the solution. Use hydrochloric acid ... Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear MW 25,000 Da Heat-inactivated...Corning 430167 15 mL conical tubes, VWR 21008-216 Hydrochloric acid Sodium hydroxide 0.22 μm polyethersulfone... will be basic and will need adjustment with hydrochloric acid first. Pro-Tip The pH of this solution ...mL conical and an appropriate amount of 25 mM chloroquine to the conical so that the final concentration...transfection mixes are added) will be 25 uM of chloroquine. After the incubation, add the DNA:PEI-Max mix...mix to the conical containing the media and chloroquine and mix well. Gently aspirate the media out of...