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Addgene
Showing: 81 - 100 of 113 results

  3. Brain Initiative Collection

    Type
    Collection
    ...AAV9 pAAV_hSynapsin_psychLight2 To generate Adeno-Associated viruses for expression of psychLight2 under...

  5. Typing CRISPR Systems

    Type
    Blog Post
    ...References Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine...

  6. CRISPR Guide

    Type
    Collection
    ...alternatives Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine...Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V., Donghia..., O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., Van Der Oost, ...

  8. Optogenetics Plasmid Collection

    Type
    Collection
    ... widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that...

  9. Brzezinski Lab CRISPR Collection

    Type
    Collection
    .... Usage Guide sequences can be designed using Benchling or using similar design tools and ordered as standard...

  11. Synthetic Biology - Overview

    Type
    Collection
    ...Balazsi Matthew Bennett Carolyn Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins Tom...

  12. Microbiology Resources

    Type
    Collection
    ...Burkholderia sp. Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus...

  13. Qi Lab CRISPR Page

    Type
    Collection
    ...inducible dcas9 gene on a p15A vector with chloramphenicol resistance. The second plasmid ( pgRNA_bacteria...

  14. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Marraffini 42876 pCas9 BsaI E. coli, S. pneumoniae Chloramphenicol yes, cut Marraffini 44251 pgRNA-bacteria BBa_J23119...

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Knockout Human Garnett 3rd 2 37,722 Green monkey (Chlorocebus sabaeus) sgRNA library 178284 Knockout Green ...

  17. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...mL RNase A TE or water-saturated phenol-chloroform Chloroform 100% ethanol or isopropanol 90% ethanol ...: Perform phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction removes...Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the...volume of chloroform to the recovered aqueous DNA layer. Repeat steps 2-4. Note: Phenol-chloroform is a hazardous...aqueous DNA sample. Pro-Tip Water-saturated phenol-chloroform can be used if TE-saturated is not available....without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant after step 6 and...

  18. General Transfection

    Type
    Protocol
    ...56 °C for 30 min. 25 mM chloroquine diphosphate Dissolve 0.129 g of chloroquine diphosphate salt into 10...slowly add hydrochloric acid until the solution clears. Check the pH of the solution Use hydrochloric acid ... Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear MW 25,000 Da Microcentrifuge....X 10 cm tissue culture dish, Corning 430167 Hydrochloric acid Sodium hydroxide 0.22 μm polyethersulfone... will be basic and will need adjustment with hydrochloric acid first. Pro-Tip The pH of this solution ...add 10 mL fresh DMEM complete containing 25 µM chloroquine diphosphate and incubate ~5 h For 10 mL of DMEM...DMEM complete, add 10 µL of 25 mM chloroquine diphosphate. Dilute 18.9 µg of DNA into 500 µL of Opti-Pro...

  19. Kit Free RNA Extraction

    Type
    Protocol
    ...are also included: using Isopropanol or Lithium Chloride. This protocol was adapted from (Link opens in...Water-saturated Phenol 2 M Sodium Acetate pH 4 Chloroform/Isoamyl alcohol (49:1) 75% Ethanol RNase-free...for precipitation step, Option A) 7.5 M Lithium Chloride (for precipitation step, Option B) Glycogen (Optional...phenol, mix thoroughly by inversion. Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) and then shake vigorously...(s). You can use either Isopropanol or Lithium Chloride for this step. Isopropanol (Option A): Add 1 volume...aqueous layer. Incubate at -20°C for 1 hour. Lithium Chloride (Option B): LiCl selectively precipitates RNA ...from the homogenized sample(s). Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) per 1 mL of TRIzol® used...

  20. Lentivirus Production

    Type
    Protocol
    ... add hydrochloric acid until the solution clears. Check the pH of the solution. Use hydrochloric acid ... Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear MW 25,000 Da Heat-inactivated...Corning 430167 15 mL conical tubes, VWR 21008-216 Hydrochloric acid Sodium hydroxide 0.22 μm polyethersulfone... will be basic and will need adjustment with hydrochloric acid first. Pro-Tip The pH of this solution ...mL conical and an appropriate amount of 25 mM chloroquine to the conical so that the final concentration...transfection mixes are added) will be 25 uM of chloroquine. After the incubation, add the DNA:PEI-Max mix...mix to the conical containing the media and chloroquine and mix well. Gently aspirate the media out of...
Showing: 81 - 100 of 113 results