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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 443 results for: DUR

Showing: 81 - 100 of 443 results

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...mutant cells were treated with WNV at a dose and duration sufficient to kill all control cells and surviving...mutant enables transient suppression of DNA repair during oligonucleotide integration, allowing MAGE in otherwise...polyproteam can be eluted using maltose. This procedure allows for stable and directional protein fusion...need for PCR or additional molecular cloning steps during plasmid assembly. This toolkit reduces the expense... of these genes were investigated for functions during infection of human cells, and one of them called...

  3. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ..., the vector may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try...result of plasmid integration via recombination during transfection, and does not necessarily indicate...Yezzi, and Barbara R. Young. "Recombination events during integration of transfected DNA into normal human...

  4. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...animal’s skull using dental cement (a similar procedure as implanting a guide cannula). A fiber optic ...cable to the ferrule implant must be maintained during the entire protocol, otherwise optical stimulation...that one laser can stimulate only a single animal during the course of an experiment. There are a number...

  5. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...abilities we often hear that scientists should acquire during their PhD and postdoctoral training. Addgene executive...different types of people both above and below you. During my time at the NPA, there were many changes to ... hands-on- and role-playing exercises were held during the weekend. I attended multiple CLIs and benefited...

  6. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...other side), you can check by using two membranes during your transfer instead of one. If you see signal...you had air trapped between the membrane and gel during the transfer, while dark splotches could indicate... mixed blocking buffer, or insufficient rocking during incubation steps.  Many errors can arise from technical... If you’re seeing high background or low signal during the imaging process, try adjusting your exposure...

  7. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...contamination, use appropriate decontamination procedures (Korte et al., 2021) for all disposable items... iodixanol) using a high-speed ultracentrifuge. During the spin, the virions will form a band due to their...release of functional vector into the culture medium during adeno-associated virus manufacturing. Human Gene...

  8. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ... steps, until its connection with DNA is broken during cross-link reversal (not shown in diagram). Cross-links...in chromatin conformation, like those that occur during development and cellular differentiation. Using...the Xu Lab tracked enhancer-promoter interactions during erythroid differentiation. CAPTURE 2.0 captures...

  9. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ... Protocol Pages Agarose cloning method procedure The procedure (illustrated in the YouTube video below...isolate colonies of antibiotic-resistant cells; during the time required to grease, place and fill a dozen...

  10. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...producer cell membrane, and acquired as an envelope during exocytotic budding, as the viral particle emerges...transfer of RT, which would actually occur in step 2 during uncoating. After uncoating, reverse transcription... integrated by the viral integrase protein (IN) during step 3 (King et al., 2008) (Hu & Temin, 1990). ...

  11. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ... caused by loss of protein during digestion, or masking of epitopes during sample anchoring and gel polymerization...occurs, due to a reduction in target concentration during expansion. One group has found that using Fab fragment...

  12. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...to follow an exponential decay model, with dips during weekends and significant holidays. Figure...likelihood by the corresponding factor 𝜙z if t is during event z. The "events" we considered for our model... Addgene blog Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find plasmids...

  13. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...want to maintain the highest quality and accuracy during the QC process and therefore put in a lot of effort...by synthesis, but modifies the original Sanger procedure to improve throughput by increasing the number...5467. https://doi.org/10.1073/pnas.74.12.5463  Shendure, J., & Ji, H. (2008). Next-generation DNA sequencing...

  14. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...cross-react with samples, eliminating them from the procedure removes one potential source of cross-reactivity...antibodies that work well together. Moreover, the procedure is longer than alternative ELISA methods, especially...peptide. The positive control will confirm that the procedure is working and should always be included.  For...

  15. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...creativity is absolutely a transferable skill developed during PhD training. Creativity is required to develop... need ahead of time and mute your notifications during. (Boyes, 2019) If you’ve taken the time to do this... were asked to create a device to help students during an interview. When they were instructed to use ...

  16. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...system to partition themselves to each daughter cell during cell division. They don’t simply rely on chance...CBP), and a partition NTPase (Schumacher, 2012).  During cell division, CBPs bind to the centromere-like...replication that occurs for some types low copy plasmids during cell division simply does not allow for enough ...

  17. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...contains a plasmid with a T-DNA (transfer-DNA) region. During infection this T-DNA region is transferred into...transformation and gene expression can be tracked. During a transient leaf transformation, the engineered...other aspects of the transient transformation procedure (Mortensen et al., 2019). Our method might provide...

  18. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...the host genome via the viral proteins packaged during production (read our blog post on the retroviral...time, IDLVs are ideal. As the episomes are lost during cell division, the expression of the delivered ...activity yet fail to integrate viral DNA efficiently during infection. Journal of Virology, 70(2), 721–728....

  19. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...not need to use a separate fix/perm cytosolic procedure in that case.     Figure 3: For intracellular... used. These dyes are incorporated into the DNA during DNA synthesis and then diluted with subsequent ...negative live cells can still be easily gated on during the analysis. Conclusion Congratulations! You made...

  20. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...progress), and I know that I’ve blocked out time during my day according to my priorities, I have a pretty...-world example, let’s say I’ve blocked out time during my day to write a blog post, edit other blog posts...leave your phone in your bag on “Do Not Disturb” during the times you’ve blocked out for other activities...
Showing: 81 - 100 of 443 results