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Showing: 81 - 100 of 771 results
  1. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ... CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods. Researchers...epitope tagging). NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received...
  2. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...become clear that the power of this editing platform lies in the diversity of nucleases and their applications...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...
  3. Recombinases AAV Preps

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    Collection
    ...-DIO-FLPo-WPRE-hGHpA EF1a none 1, 2, 5, 8, 9, rg* Li Zhang 75469 pAAV-EF1a-Flp-DOG-NW EF1a none 1 Connie...
  4. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...13226-x Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Donohoue, P. D....
  5. Design Tips for Prime Editing

    Type
    Blog Post
    ...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ...
  6. Hot Plasmids - November 2023

    Type
    Blog Post
    ...in single cells. Find iPAK4 plasmids here! Lin D., Li X., et al. (2023). Time-tagged ticker tapes for intracellular...whole-brain reference atlas efforts, including a list of validated antibodies, on their webpage: mAb3D...
  7. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...PubMed Central PMCID: PMC1462937. Dietzschold, B., Li, J., Faber, M., and Schnell, M. (2008). Concepts ...Sorensen, S.A., Slaughterbeck, C.R., Wakeman, W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K....advantage of AAV vectors is that there are well-established protocols for their production and purification...AAV1, -2, -5, -7, -8 (Taymans et al., 2007), AAV1 (Hollis et al., 2008) AAV6 (Towne et al., 2010), AAV8, ...PMID: 19098898. PubMed Central PMCID: PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R...
  8. mTOR Pathway

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    Collection
    ...PMID: 22500797 . Rapamycin: one drug, many effects. Li J, Kim SG, Blenis J. Cell Metab. 2014 Mar 4;19(3)...Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m ammalian t arget...available plasmids for the gene, or browse the gene list below. For multiple isoforms or subunits, individual...David Sabatini . Return to top mTOR Pathway - Gene List Click on a name to find available plasmids for the... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...
  9. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn, ...and in ternary complexes with other proteins, establishing CRBNmidi as a promising tool for future structure-guided...
  10. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...-4  Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F (2014) In vivo interrogation... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...Cas12b and CasX. While there are no papers yet published that deliver these Cas proteins with AAV, at just...
  11. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This...
  12. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...academic research environment. The “how many?” question lies at the base of all these questions as there is not... career development. Publishing Panel The Early Career Researchers in Publishing Panel brought in editors...Outcomes University websites do not currently publish career outcomes for PhDs or postdocs. Having this...Path Panel and the Early Career Researchers in Publishing panel, focused on showing researchers that they... PIs and all gave advice on how to succeed in publishing in the current academic environment. Some great...writing skills will help you in your academic publishing goals and make you a better communicator overall...have any questions or concerns throughout the publishing process. The diversity panel - Equity and inclusion...
  13. Plant Plasmids and Resources

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    Collection
    ...editing across crop species and genotypes - Zhenghe Li. A robust CRISPR/Cas9 system for convenient, high-efficiency... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...
  14. CRISPR History and Development for Genome Engineering

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    Collection
    ..., Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang...Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR, Aryee MJ, Joung JK. 2015....mammalian cell culture. Five years later, PubMed lists more than 6,300 CRISPR-related publications, many...
  15. Zhang Lab CRISPR Page

    Type
    Collection
    ...DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini...CRISPR-Cas9. Swiech L, Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . ... of Cas9 in vivo and ex vivo , the Zhang Lab established Cre-dependent and constitutively expressing Cas9...
Showing: 81 - 100 of 771 results