We narrowed to 236 results for: Library
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TypeBlog Post... has been working on developing CRISPR pooled libraries for use in genetic screens and has contributed...Addgene Find CRISPR resources Find CRISPR pooled libraries Learn about other members of the Addgene Advisory...
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Suggest A Plasmid You'd Like to Find at Addgene
TypeBlog Post...for us to reach everyone. Therefore, to make our library more useful to our community, we’re asking you ... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...human miRNAs. Weiss’s collection consists of a library of reporter plasmids, each containing two fluorescent...used for high throughput screens using pooled libraries. Find the CHIME plasmids at Addgene. LaFleur et... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...looking ahead, we’re starting to build saturating libraries of all possible mutations in many major cancer...our Cancer Colleciton Pages Use the ClonTracer Library for Lineage Tracing Learn About Cancer Immunotherapies... -
Hot Plasmids - February 2022
TypeBlog Post...genome-wide library capable of performing similarly to Cas9 screens in a compacted library. Overall, Cas12a... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...both for screens (using pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses... O, Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11... -
It’s CRISPR Week at Addgene!
TypeBlog Post...CRISPR - from nickases to base editing to pooled library screening. It also has information for planning... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...Plasmids: Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale...that can be used to generate random mutagenesis libraries or to insert heterologous genes, reporters, or...Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems have been developed...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering... plasmids, or choose from a growing number of libraries containing pre-constructed standardized modules... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...so that they are easily distinguished. The UCLA Library offers some Powerpoint based templates with different...difficult to read. The Thompson Rivers University Library provides useful tips on how to craft this type ... -
Plasmids 101: Modular Cloning
TypeBlog Post...variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with a relevant...certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally, but these... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...pooled library from Keith Joung’s and Benjamin Kleinstiver’s labs are now available. These libraries encode... -
New Videos: Addgene Lab Tips
TypeBlog Post...Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol Pages Find Molecular Biology... -
Twenty Years of Sharing Science!
TypeBlog Post...DNA-based materials, including our popular kits and libraries. This DNA-based materials repository contains ... -
8 Great Depositor Research Stories
TypeBlog Post...paper uncovering ancestral bias in CRISPR guide library design. dCas9-directed DNA Demethylation: Guest... -
Hot Plasmids February 2024
TypeBlog Post...multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023). They ...investigating NHP brains. Figure 3: Overview of library generation and screening that led to identification... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...-Cas9 genome engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823... -
Antibody Data Repositories and Search Engines
TypeBlog Post... Data repository From the Frederick National Library for Cancer Research Human Luekocyte Differentiation... -
CRISPR Cheat Sheet
TypeBlog Post...increase or decrease expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target multiple... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...PHP.B was created by first generating a diverse library of AAV9 mutants that had a small randomized peptide... loop inserted in the AAV9 capsid. This mutant library was then screened in vivo in C57BL/6J mice strains... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...to implement in genome-wide screens over cDNA libraries. However, using CRISPRa to target individual genes... activator were transduced with a pooled sgRNA library and were subsequently cultured for nearly two weeks...