...human miRNAs. Weiss’s collection consists of a library of reporter plasmids, each containing two fluorescent...used for high throughput screens using pooled libraries. Find the CHIME plasmids at Addgene. LaFleur et...

...genome-wide library capable of performing similarly to Cas9 screens in a compacted library. Overall, Cas12a...

...looking ahead, we’re starting to build saturating libraries of all possible mutations in many major cancer...our Cancer Colleciton Pages Use the ClonTracer Library for Lineage Tracing Learn About Cancer Immunotherapies...

...both for screens (using pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses... O, Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11...

...CRISPR - from nickases to base editing to pooled library screening. It also has information for planning...

...Plasmids: Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale...that can be used to generate random mutagenesis libraries or to insert heterologous genes, reporters, or...Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems have been developed...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering... plasmids, or choose from a growing number of libraries containing pre-constructed standardized modules...

...pooled library from Keith Joung’s and Benjamin Kleinstiver’s labs are now available. These libraries encode...

...variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with a relevant...certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally, but these...

...so that they are easily distinguished. The UCLA Library offers some Powerpoint based templates with different...difficult to read. The Thompson Rivers University Library provides useful tips on how to craft this type ...

...Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol Pages Find Molecular Biology...

...DNA-based materials, including our popular kits and libraries. This DNA-based materials repository contains ...

...about each library can be found on the individual library page. Find Pooled Libraries of Interest...pneumoniae Veening N/A 1 1,499 MusCK Library 174196 (Library A) 174197 (Library B) 1000000178 (A & B) Knockout... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function...amplify the pooled library and the use of Next-Generation Sequencing (NGS) to confirm library representation...experiment, the next step is to pick a library. The pooled CRISPR libraries in Addgene’s collection differ based...a given CRISPR library are unique to the genome of particular organism, and the library is only compatible...filtered list of the CRISPR pooled libraries in Addgene's collection. Library Type Activation Barcode Knockout...

...Amplifying and Using the Library Once you receive your library from Addgene, check the library information to see...Overview of what pooled libraries are; how to amplify and use a pooled library; and what types of screens... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled Libraries Jump To: ...: Pooled Library Guide Introduction Library Amplification Types of Screens Resources An Introduction to... to Pooled Libraries Pooled libraries represent a powerful tool for forward genetic screening, or identifying...pooled libraries are single preparations of many different plasmids. Plasmids within a given library have...sets. In cDNA libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid...

...paper uncovering ancestral bias in CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest...

...knockout pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone...In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses...Service page . Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes involved...Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178 ...73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting...Root 73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting...Root Human activation pooled libraries 92379 Human CRISPRa sgRNA library Calabrese in backbone XPR_502...

...multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023). They ...investigating NHP brains. Figure 3: Overview of library generation and screening that led to identification...

...-Cas9 genome engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823...

... Data repository From the Frederick National Library for Cancer Research Human Luekocyte Differentiation...

...increase or decrease expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target multiple...

...Pooled Library 111704 Mode #1 Library Pooled Library 111705 Mode #2 Library (14-3-3β) Pooled Library 111706...Mode #2 Library (14-3-3σ) Pooled Library 111707 Mode #2 Library (NEDD4 WW2 domain) Pooled Library 111708...Pooled Library 188526 iSPI_pSer_Subpool#1 Pooled Library 188527 iSPI_pSer_Subpool#2 Pooled Library 188528...Pooled Library 188529 iSPI_pSer_Subpool#4 Pooled Library 188530 iSPI_pSer_Subpool#5 Pooled Library 188531...Pooled Library 188532 iSPI_pSer_Subpool#7 Pooled Library 188533 iSPI_pSer_Subpool#8 Pooled Library 188534...iSPI_pSer_Subpool#9 Pooled Library 188535 iSPI_pSer_Subpool#10 Pooled Library 188536 iSPI_pSer_Full_library Strain 68306...phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite library ( 111704...