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We narrowed to 222 results for: Library

Showing: 81 - 100 of 222 results

  2. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...the past couple months: A new lentiviral CRISPR library from Alec Kimmelman targets mouse metabolic genes...to wild type. A new genome-wide CRISPR knockout library from Xiaole Shirley Liu's lab targets all mouse...

  3. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...shows how you can use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening experiments...experiments. Get the full details on how to make these libraries from the post and the associated publication. ...

  5. Plasmid Collections

    Type
    Collection
    ...Pathways & ORFs Synthetic Biology Pooled Libraries Pooled libraries are a set of plasmids all built with ...region. Click here to view all pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor...curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, ...

  6. COVID-19 Resources

    Type
    Collection
    ...Membrane Proteins Return to top Pooled Libraries Pooled Library Name Type PI Description SARS-CoV-2 Spike...Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead Libraries of Spike (S) Ectodomain and...Binding Domain Deep Mutation Scanning Library Screening Jesse Bloom Library of Spike protein RBD mutations for...-Saturation Mutagenesis Libraries Screening Jesse Bloom Tyler Starr Libraries of variant Spike protein...Mammalian Targets General Plasmid Tools Pooled Libraries Other Resources Image credit: Maya Kostman for...Articles (Link opens in a new window) Wiley Online Library - Covid-19: Novel Coronavirus Content Free to Access...

  7. Plasmid Cloning by PCR

    Type
    Blog Post
    ...plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient to give...you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate the PCR product Isolate ...

  8. Distribution to Industry

    Type
    Collection
    .... Pooled Libraries Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten...Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering and MTA Information Featured... Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of...human TP53 exon 5–8. Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created for research ...

  9. Zhang Lab CRISPR Page

    Type
    Collection
    ...Return to top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for...genome-wide human SAM library SAM can be combined with a human genome-wide library to activate all known...10.1038/nmeth.3047. PubMed . Return to GeCKO Library SAM Library Genome-scale transcriptional activation by... performing genome-wide knockout screens. The libraries are available in two formats: 1 vector system ...lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO Library CRISPR page for more information. Full references...transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic Activation Mediator (SAM...more information, please visit the Addgene SAM library page . Targeted gene activation using SAM There...

  10. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled Library - Toxoplasma gondii CRISPR...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding...

  12. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ... has been working on developing CRISPR pooled libraries for use in genetic screens and has contributed...Addgene Find CRISPR resources Find CRISPR pooled libraries Learn about other members of the Addgene Advisory...

  14. CRISPR References and Information

    Type
    Collection
    ...Addgene blog. CRISPR Pooled Libraries Addgene's collection of CRISPR pooled libraries for large-scale functional...protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene Church gRNA...plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's downloadable ...Editing Toolkit) (Link opens in a new window) A library of various python functions for the purpose of ...control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2...pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are also available for human and mice PDF, 276...

  16. Hot Plasmids - February 2022

    Type
    Blog Post
    ...genome-wide library capable of performing similarly to Cas9 screens in a compacted library. Overall, Cas12a...

  18. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...both for screens (using pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses... O, Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11...

  19. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...CRISPR - from nickases to base editing to pooled library screening. It also has information for planning...

  20. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Plasmids: Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale...that can be used to generate random mutagenesis libraries or to insert heterologous genes, reporters, or...Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems have been developed...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering... plasmids, or choose from a growing number of libraries containing pre-constructed standardized modules...
Showing: 81 - 100 of 222 results