...this post: Improved voltage indicator: JEDI-2P Novel class of light-gated potassium channelrhodopsins...https://doi.org/10.1016/j.cell.2022.07.013.   Novel class of light-gated potassium channelrhodopsins...labs of Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins...fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that HcKCR1 and...photoactivation of HcKCR1. Images adapted from Govorunova et al. 2022.     HcKCRs represent a long-...performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins are natural...

...mammalian research: 𝛄-retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). While each...higher end. Adeno and adeno-associated viruses Adenoviruses infect non-dividing cells but they do not permanently...integration causing mutagenesis in the host. Adenoviruses can deliver relatively large cargo inserts (...non-dividing cells. AAVs primary advantage over adenovirus is their low immunogenicity, which means they...cells Insertional Mutagenesis Possible Adenovirus Remains Episomal (Little Risk of Insertional... adenovirus...

...Dumas, Lercher, Spicer, & Davis, 2015). By using a novel aminoacyl tRNA synthetase/tRNA pair, a researcher...engineered into a desired site. During translation, the novel tRNA/unnatural amino acid finds the UAG codon in...continually being developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies...experimental design lends itself to creativity and innovation, valuable commodities in any NIH grant application... understanding of your protein that can lead to novel insights about its biology and its behavior. See...in Boston, MA. His interests include developing novel fluorescent tools for labeling reagents and structurally... of ryanodine receptor type 1 structure using a novel site-specific labeling method." PloS one 4.10 (2009...

...did we get here in the first place? How CRISPR innovation affected software development CRISPR, like the...it, offers an interesting view into how rapid innovation affects the research community. I’ve likened ...That’s just 3 to 4 years ago. It is a bubble of innovation (a piñata), and software development has caught...separate sgRNA design tools into database and de novo solutions. Database tools allow us to view and get...community in order to succeed. With respect to de novo sgRNA design, I again separate it into two categories...future of CRISPR software is promising. Rapid innovation in this space has put us in a good position to...

...produce, so most labs opt to purchase adenoviruses from dedicated adenoviral production labs or companies. Production...available. The viruses Lentiviruses, AAV, and adenoviruses can all be produced in mammalian cells. However...make lentiviruses and AAV in-house. High-titer adenoviruses are difficult and time consuming to produce,...transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. The FASEB Journal...

...Non-integrating iPSC delivery methods Viral Vectors Adenovirus: Adenoviral vectors infect dividing and nondividing...verify excision didn’t introduce mutations Adenovirus Non-integrating Low Medium Generates transgene-free...polycistronic transgene or with four different adenoviruses, each expressing one factor. These vectors don...less likely to cause insertional mutagenesis, adenoviral vectors are considered a safer way to express...Minoru Iijima, Manami Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression Mediated...

...replication when copy number in the cell falls too low (Novcik, 1987).  Antisense RNAs encoded on the plasmid ...plasmid for replication or partitioning machinery (Novick, 1987). In cases where one plasmid outcompetes ...incompatibility to combat antimicrobial resistance. Using novel plasmids based partly on plasmid incompatibility.../j.1472-765x.2008.02426.x Pubmed PMID: 19241516 Novick RP (1987) Plasmid incompatibility. Microbiology... 25873913 Uraji M, Suzuki K, Yoshida K (2002) A novel plasmid curing method using incompatibility of plant...

...we’ve seen so far!). They started by designing a novel topology, then iteratively generated 3D models and...Although Top7 was designed simply to be stable and novel, the principles that created it have been applied...the Baker Lab and others. The introduction of de novo designed proteins also helped researchers understand...Stoddard, B. L., & Baker, D. (2003). Design of a Novel Globular Protein Fold with Atomic-Level Accuracy...

...animal testing and modifications. The premise of the novel involves Dr. Moreau who creates human-animal hybrids... writer Jack Williamson who used it in his 1951 novel Dragon’s Island. In 1976, the world’s first genetic...against a genetically engineered superhuman Khan. The novel, Inferno (2013) and the movie version (2016) stars...editors which can help accelerate the engineering of novel base editor proteins. She is particularly interested...Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Meyer BJ (2011) Targeted Genome Editing Across...

... changed its name from Novophage to Sample 6. To learn more about Novophage's successful transformation...Koeris and Lu founded the biotech startup company, Novophage. As you'll hear Dr. Koeris explain however, the...firm, Flagship Ventures, Koeris helped change Novophage's focus. The company went from developing therapeutics...

...our partnership with the Institute for Protein Innovation (IPI), we expanded a growing collection of thoroughly...enter 2025, your feedback continues to guide our innovation.  In response to a demand for more AAV viral ...extensive plasmid database — because we believe that innovation flourishes when data are shared. The scientific...look ahead, I am excited to continue to grow and innovate with your help and guidance. Every plasmid shared...

.... https://doi.org/10.1101/2020.05.04.076067    Novel fluorescent enzyme biosensors provide direct, specific...optimization. Claudia Vickers’s lab has designed novel SL fluorescent biosensors by integrating a SL-insensitive...parasitic Striga hermonthica SL receptor HTL7. This novel type of biosensors could be used in high throughput...As a result, agricultural applications of these novel biosensors could have a major economic impact.   ...

...of the company.  Ultragenyx’s goal is to develop novel products for the treatment of rare and ultra-rare...was the Head of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development...are less common, they can be very impactful. At Novartis, Stegmeier deposited the ClonTracer Barcoding ...built create inefficiencies that ultimately make innovation and science slow,” Vandenberghe says. “The continuous...

..., founding bioethicist of Rogue Bioethics. On November 25, the MIT Technology Review dropped a bombshell...forward” is the June 2012 report on the ethics of “Novel techniques for the prevention of mitochondrial DNA...Nuffield Council began their research into both novel and previously established ethical issues raised...industry to inform her work on emerging issues in novel technologies, including self-driving vehicles, synthetic...

...activity after ischemic brain injury Dr. Gohar Tsakanova, Institute of Molecular Biology of the NationalAcademy...Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior Researcher who investigates the molecular...become blocked, resulting in reduced blood flow. Novel treatment options, as well as non-pharmacological...two-photon laser scanning microscope will allow Tsakanova to image cortical neurons and study changes in...

...CRISPR–Cas9 activity switching via a novel optogenetic variant of AcrIIA4 (CASANOVA). As shown in the figure below...release of Cas9. A noteworthy application of the CASANOVA system was its use in light-mediated CRISPR labeling...minutes and red foci formed 20-40 min later. The CASANOVA system is versatile in that it can be adapted ...Light-Dependent Anti-CRISPR Bubeck et al., 2018 CASANOVA LOV2 from A. Sativa AcrIIA4 anti-CRISPR protein...

...have been investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles... AAV was first discovered as a contaminant of adenovirus preparations. It is an icosahedral, non-enveloped... requires co-infection of helper viruses like adenovirus for productive infection. AAV vectors are engineered... have slight variations in their viral capsids. Novel forms of AAV capsids are discovered frequently and...

...Commun. 2017. PubMed PMID: 28646204. soCoChR: A novel opsin that enables single-cell optogenetics Article...system. Taken together, Shemesh et. al. describe a novel system for the precise mapping of neuronal networks...developed two new FLIM-FRET acceptors, ShadowG, a novel dark green fluorescent protein (GFP) with an absorption...absorption peak of 486 nm and ShadowY, a novel dark yellow fluorescent protein (YFP) with an excitation...

...research? Do you have innovative tools? A novel dataset? Unique expertise? A novel hypothesis? Part of ...problem and then explain why it’s significant and innovative. For example, "while it is possible to grow liver...

... Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, ...Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (...