Skip to main content
Addgene
Showing: 81 - 100 of 286 results

  1. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann...10.1016/j.cell.2014.02.001  Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O,...

  3. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...Plasmid #141386-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein...or #211757 (middle panel). Samples were also immunoblotted against vinculin as a loading control (right...

  4. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...antibody-based assays, like an ELISA or Western Blotting, but it allows you to visualize a target of interest... antibody application in The Basics of Western Blotting Resources on Addgene.org Find plasmids encoding...

  5. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...quizzically and ask why I didn't just say that? Bottom line is to appreciate that your audience needs ...did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health...

  6. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...detection system, as is commonly used in western blotting or ELISAs.  A popular method for indirect labeling...host species reactivity. Just like for western blotting, a primary epitope-targeting antibody can originate...

  7. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ... faculty candidate. Sweaty palms. Heart racing. Cotton mouth.The candidate may find it difficult to answer...region you’ll be working. At this point, your budget bottom line costs may be rather high. That’s normal, because...

  8. A Control for All Seasons

    Type
    Blog Post
    ...controls, which we will briefly touch on.  Western blotting When comparing samples in different lanes in a... P. H., et al. “Goals and Practicalities of Immunoblotting and Immunohistochemistry: A Guide for Submission...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...recently deposited with Addgene by Dr. Mark Prescott. Dr. Prescott and Dr. Rod Devenish (both at Monash University...use. The plasmids were validated by both Western blotting and immunohistochemistry, and are summarized in...

  11. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...piggyBac was discovered in the cabbage looper moth (Potter et al., 1976). Its target site is TTAA and unlike...genome.   References and Resources References Feschotte C, Pritham EJ (2007) DNA Transposons and the Evolution...

  13. Typing CRISPR Systems

    Type
    Blog Post
    ...H., Horvath, P., Moineau, S., Mojica, F. J. M., Scott, D., Shah, S. A., Siksnys, V., Terns, M. P., Venclovas...Makarova, K. S., Koonin, E. V., Cheng, D. R., & Scott, D. A. (2018). Functionally diverse type V CRISPR-Cas...

  14. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ... and Continue to Next Step" (blue button at the bottom of Figure 6) or you can click the "Save" button...Be sure to press the white "Save" button at the bottom of the page before moving onto a different page...

  15. Antibody Guide

    Type
    Guide
    ...resulting light emissions. In FACS, cells are then slotted in a specific direction based on the emission(s...

  16. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...protein-based buffers, so here’s a quick tip: Keep a bottle of chemical buffer around, which works well and...Stacked Film Exposure Improve Sensitivity in Western Blotting. Journal of Pharmacological and Toxicological ...

  18. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ... plasmids since 2004. In that time, our lab has gotten a lot experience in processes including automated...
Showing: 81 - 100 of 286 results