We narrowed to 238 results for: SPL

1. RaPID Detection of RNA-protein Interactions

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   Blog Post

   ...Interactions with Split-BioID Find resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids...

2. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...colleagues are working on developing new surface display vectors to improve expression and trafficking of...: Schematic of iGluSnFR3 (left). GluSnFR3 in pMinDisplay (PDGFR anchoring domain), in electroporated in...

3. Hot Plasmids and Viral Preps - January 2021

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   Blog Post

   ...generated a photosensitive Cre, Opto-Cre-Vvd, by splitting and joining the protein regions with Vivid (Vvd...low and high-intensity blue light. Unlike other split-Cre systems which can take up to 24 hours to cut...

4. The AAVantages of AAV in CRISPR Screens

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   Blog Post

   ...cells with the CRISPR library in vitro before transplanting them into mice. For in vivo screens, mice are...another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog like SaCas9. You...

5. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   ...vitro (see figure 1B in Terskikh et al.) and even displays predictable kinetics over 14 hours of expression... table: Fast-FT, Medium-FT, and Slow-FT. These display progressively longer blue fluorescence maturation...

6. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   ...Mapping antibody-escape mutations with the yeast display library. From Starr et al Science., 2021. SARS-CoV... designed so that the SARS-CoV-2 spike RBD is displayed on the yeast cell’s surface. The Bloom lab used...

7. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   Blog Post

   ...storage. What we don't have are the keyboards and displays. We don't have good ways of converting typically...is the next priority; we need the keyboards and displays for control and visualization. Addgene: What do...

8. CRISPR 101: RNA Editing with Cas13

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   Blog Post

   ...mismatch at that position. PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from...is more precise than shRNA knockdown, it still displays substantial off-target activity. By mutating residues...

9. Plasmids 101: Secondary Nanobody Toolbox

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   Blog Post

   ...penetration Less resolution due to larger label displacement Require separate incubation of 1° and 2° Must...multiple dyes Greater resolution due to lower label displacement 2° incubation can be skipped by pre-binding ...

10. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...their characterization of two Cpf1 orthologs that display cleavage activity in mammalian cells. Like Cas9...Cpf1 candidates, Zetsche et al. found two that display robust cleavage activity similar to that of Cas9...

11. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split EGFP has also been used to study...

12. Hot Plasmids: Fall 2024

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    Blog Post

    ...light-sensitive systems. Most excitingly, assembly of the split luciferase is reversible, making PILATeS suitable...., Wang, B., Prakash, M., & Dunn, A. R. (2024). Split Luciferase Molecular Tension Sensors for Bioluminescent...

13. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

    ...is a significant need for healthy livers for transplants. Growth of new livers in culture could resolve..., neither of these can substitute for a liver transplant. My lab will combine bioengineered scaffolds ...

14. Antibodies 101: Reading a Flow Plot

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    Blog Post

    ... cells correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients... recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23 Jhunjhunwala...

15. CRISPR Activation: A Practical Guide

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    Blog Post

    ...environmental triggers. This is done by creating a split dCas9 protein that can reassemble when exposed to...015-0784-0 Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and...

16. Plasmid Collections

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    Collection

    ...Library Types Barcoding CRISPR Screening Surface Display cDNA Depositor Collections A modular set of plasmids...

17. Five Popular Model Organisms, Part 2

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    Blog Post

    ...popular tools for studying frogs include egg transplants and microinjections. Injections of mRNAs, for...disease-relevant genes in such a controlled environment displays how essential the frog is as a biomedical model...

18. Hot Biosensors 2022: Year-End Roundup

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    Blog Post

    ...authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression, and sensitivity...from Twitch-2B (Matlashov et al. 2022). iGECInano displays better response kinetics and photochemical characteristics...

19. Quick Guide to Near-Infrared Fluorescent Proteins

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    Blog Post

    ...protein-protein interactions, we recommend using NIR split reporters based on miRFPs (Shcherbakova et al., ...and higher contrast than previously developed NIR split probes (Tchekanda et al., 2014, Filonov et al., ...

20. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

    ...to a number of rare genetic diseases caused by splicing defects, including ataxia telangiectasia, congenital...Exon-skipping antisense oligonucleotides to correct missplicing in neurogenetic diseases. Nucleic Acid Ther. ...