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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 30 results for: SPL

Showing: 1 - 20 of 30 results

  1. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...EcAR7 Plasmid 112031 pNAS1b split mCherry 14-3-3β ctrl Plasmid 112030 pNAS1b split mCherry NEDD4 WW2 neg ctrl...Plasmid 112029 pNAS1b split mCherry NEDD4 WW2 pos ctrl Plasmid 111883 pNAS1b split mCherry NEDD4-2 WW2 ...WW2 Plasmid 111882 pNAS1b split mCherry NEDD4 WW2 Plasmid 111881 pNAS1b split mCherry 14-3-3σ Plasmid 111880...introduced into a second expression vector encoding a split mCherry fluorescent reporter, enabling the detection...phosphosite library is fused to the N-terminal portion of split mCherry, while a phosphobinding domain is fused ...111880 pNAS1b split mCherry 14-3-3β Pooled Library 111704 Mode #1 Library Pooled Library 111705 Mode #2...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ...Creation of N-terminal Firefly luciferase fragment for split-luciferase complementation assay in plants using... of C-terminal Fifrefly luciferase fragment for split-luciferase complementation assay in plants using...desined de novo using deep-learning approaches. Split Luciferase Complementation assays can report on ...opens in a new window) : The NanoLuc® enzyme is split into Large (LgBiT; 18 kDa) and Small (SmBiT; 11 ...with LgBit and SmBit . Cluc-FKBP and FRB-Nluc : Split firefly luciferase reporter of rapamycin-inducible...rapamycin-inducible interaction. nLuc and cLuc : Constructs for split firefly luciferase complementation assays in plants...processes beyond transcription and translation. Splicing reporters from Younis et al., 2010 and Monteys...

  3. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...the AAV2 serotype. The AAV2(Y444F) serotype also displays enhanced transduction of the trabecular meshwork...Y730F. AAV2(4pMut)dHS The AAV2(4pMut)dHS serotype displays enhanced transduction of the retina following .... AAV6(dbY-F+T-V) The AAV6(dbY-F+T-V) serotype displays enhanced transduction of hematopoietic stem cells... Y731F and T492V. AAV44.9 The AAV44.9 serotype displays highly efficient transduction and lateral spread...Boye, et al. 2020. Novel AAV44.9-Based Vectors Display Exceptional Characteristics for Retinal Gene Therapy...

  4. Antibody Plasmid Collection

    Type
    Collection
    ... 2018;1827:313-334. Daniel Christ pComb3 phage display vector system for producing human antibodies Synthetic...1995). Carlos Barbas Variant of the pComb3 phage display vector system for isolation of scFv, diabody and...chicken monoclonal antibody fragments by phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81...

  5. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...drug-inducible approach may also be used. Split proteins: The recombinase is split into inactive N- and C-terminal...or search by inducible system (e.g., search for "split", "rapamycin", “tamoxifen”, or “light”): Cre Recombinase...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...cleavage frequency. Truncated gRNAs: Truncated gRNAs display less off-target activity. High fidelity Cas9s: ...Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very low off-target activity due to rationally ...without creating a double-strand break. This method displays higher efficiency than HDR-mediated editing. The...

  7. Bacterial Expression Systems

    Type
    Collection
    ...proteins of interest are fused to each part of a split FP. When the candidate proteins interact, the pieces...reconstruct the full FP. Find plasmids containing FPs and split FPs that can be fused to your proteins of interest...Makes Visualizing Protein–Protein Interactions Easy Split Fluorescent Proteins for Studying Protein–Protein...

  8. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...development of the original iRFP has resulted in a split fluorescence complementation probe used for detecting...690/713 6.2 piRFP iRFP720 702/720 5.8 piRFP720-N1 iSplit 690/713 5.3 pPAS-E and pK-GAFm Photoactivatable...

  9. AAV for Neuronal Tracing

    Type
    Collection
    ...Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant...rabies virus AAV1 Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement...

  10. p53 Pathway

    Type
    Collection
    ...PubMed PMID: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective.... PubMed PMID: 12619118 . p53 mutant mice that display early ageing-associated phenotypes. Tyner SD, Venkatachalam...

  11. CRISPR Guide

    Type
    Collection
    ... reverse transcription. The edited DNA strand displaces the unedited 5’ flap, and the resulting heteroduplex...including editors based on a second ADAR variant that displays improved specificity, and editors carrying an ... in vivo RNA imaging or to control alternative splicing. Figure 13: Overview of RNA targeting (A) and ...locus-specific targeting of long RNAs with CRISPR-Display. Nature Methods , 12 (7), 664–670. PMID: 26030444...

  12. Rett Syndrome

    Type
    Collection
    ...RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...2002. Insight into Rett syndrome: MeCP2 levels display tissue- and cell-specific differences and correlate...

  13. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc tag pPMW-attB - pUASp...expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular surface of S. ...

  14. Brain Initiative Collection

    Type
    Collection
    ...promoter Viviana Gradinaru 100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing...monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...iATPSnFR1.0 and iATPSnFR1.1 (cytoplasmic, membrane-displayed, expression in neurons, astrocytes) A genetically... 1720648115. Katalin Torok Glutamate Membrane displayed variants of fluorescent glutatmate sensors (iGluSnFR1...

  16. Immunology Research Plasmids and Resources

    Type
    Collection
    ... regulatory factor X-associated protein - SHFM1 split hand/foot malformation (ectrodactyly) type 1 DSS1...oncogene homolog A (avian) MGC131774, NFKB3, p65 SYK spleen tyrosine kinase DKFZp313N1010, FLJ25043, FLJ37489...of sevenless homolog 2 (Drosophila) FLJ25596 SYK spleen tyrosine kinase DKFZp313N1010, FLJ25043, FLJ37489...Fox Foundation is a synuclein phagemid VHH phage display library used to screen for antibodies that bind...

  17. Institute for Protein Innovation

    Type
    Collection
    ...platform (Link opens in a new window) based on yeast display technology (Link opens in a new window) and an ...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Rajat Rohatgi 107855 pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G) TARDBP CMV ALS Rajat...Rajat Rohatgi 107860 pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV) TARDBP CMV ALS Rajat... VWM disease Petr Svoboda 121165 tau exon 9-12 splicing minigene MAPT CMV Parkinson's, FTD Stefan Stamm...ALS Agnel Sfeir 169375 pET3d-SF-DCTN1-TS DCTN1 SplitFIAsH, Strep, His T7 ALS, motor neuron disease Bernd...

  19. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... reverse transcription. The edited DNA strand displaces the unedited 5’ flap and the resulting heteroduplex...

  20. Promega Plasmid Collection

    Type
    Collection
    ...of test compounds is measured by competitive displacement of a NanoBRET tracer, reversibly bound to a ...
Showing: 1 - 20 of 30 results