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...processing (Figure 1). The shRNA sequence is transcribed to produce hairpin shRNA structures that are ...typically use RNA polymerase III promoters, which transcribe small RNAs. The most common promoters are U6,... H., & Kim, V. N. (2004). MicroRNA genes are transcribed by RNA polymerase II. The EMBO Journal, 23(20...

...promoters can be used in the repressible manner described above, as well as the inducible manner detailed...30 years since the original Tet-Off system was described by Gossen and Bujard, both the promoter and transactivator...cells, and can be used in the repressible manner described above, as well as the inducible manner detailed...

...inserted in this LTR so you don’t need Tat to transcribe the viral genome in producing cells. Currently...process is to test several protocols that have been described (protocol 1, protocol 2, protocol 3) to find the...quantify viral RNA. All of these methods have been described in a previous Addgene blog Once you know your ...

...Plasmid #52962) was a gift from Feng Zhang and is described in Improved vectors and genome-wide libraries ...was a gift from John Doench & David Root and is described in Rational design of highly active sgRNAs for...were gifts from John Doench & David Root and are described in Optimized sgRNA design to maximize activity...

... been described in this blog. If your data does not meet the assumptions for the tests described above...error rate than the multiple comparison tests described above. Violated assumptions and statistics Statistical...

...system is much more potent than the in vitro-transcribed single guide RNA (IVT sgRNA) approach (Dewari...comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus synthetic cr:tracrRNA. Cells...step by step protocol for the csRNP method is described in our recent article (Dewari et al. 2018), however...

...after the piñata burst, value was subjectively ascribed relative to the basic needs of each individual...There are 8 major categories found in the table, describing everything from basic to advanced functionality...Software design elements that fall into this category describe how the user is expected to interact with the ...

...in practice integration can occur. Okita et al describes a protocol for generating iPSCs by plasmid transfection...Methods RNA Delivery: For this approach, in vitro transcribed RNAs are serially transfected using a cationic...transduction, such as the polyarginine peptide described in Zhou et al and Kim et al. This is considered...

...an alternative method, called STARR-seq (self-transcribing active regulatory region sequencing) that tests...assay. In their recent publication, Kroeze et al. describe their enhanced TANGO arrestin recruitment assay...When used in combination with their previously described SpyTag/SpyCatcher pair, multiple protein fusions...to fuse the AID tag to endogenous proteins. As described in their recent Cell Reports paper, the Kanemaki...

...your experiment* Functional data, if the report describes a functional vector such as a channelrhodopsin...site as well as the target region, if the report describes a retrograde serotype An abstract that summarizes...

...researchers at Japan’s Hiroshima University who described their new tool in Scientific Reports in June. ... gap. As Sakuma and colleague Takashi Yamamoto describe in their recent study, the construction system...

...characteristic radiance for some time – there are records describing them dating back to the first century A.D. – ...mechanistic understanding of GFP’s function. His lab described the protein’s structure and also discovered many...

...Jessica Sacher from the University of Alberta and describes a technique you can use to find phage proteins...molecular biology tool, P1 phage. 2) The post describes an ingenious but simple technique to screen for...

...supporting the tools in our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub... to learn more about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi,...

...communication between all members of a team. As described here in a 2012 Intuit blog post, a study coming...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...

...updated! Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene...

...Most Addgene PhD level job descriptions clearly describe that the roles do not involve bench research. ... way to a hiring manager. Learn to confidently describe your strengths in your resume and don't be shy...

... greater than any fluorescent protein earlier described. “Until our proteins, there were lots of fluorescent...illumination with far-red light. As the researchers described in Nature Communications last year, “The capability...

...Two new human lentiviral CRISPR library systems described in companion papers in Science in December were...Additionally, a concurrent Nature Biotechnology article describes the development of a mouse lentiviral CRISPR library...

... and variable spacer sequences. The array is transcribed and processed into crRNAs, each including one...from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from the CRISPR array and include a spacer and...