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Showing: 81 - 100 of 618 results

  1. Engaging with science and society at pgEd

    Type
    Blog Post
    ...ended up at pgEd, what I do there, and how you can set yourself on a path towards a similar position. How...speak about our experiences in a variety of other settings, including “conventional” academic venues such... to take care of the organizational logistics. Setting yourself on the path to science policy and science...

  2. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...Harvard Medical School, and the Doudna Lab at Berkeley set out to decrease the off target effects of CRISPR/...,3). Stable strand separation is maintained by 2 sets of interactions : 1. Interactions between the non-target...off target effects in applied and/or therapeutic settings. Will combining the different mutations found ...

  3. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...searching and reading a regular part of your routine. Set a goal to read a certain number of papers each week...up with the literature in your field of interest. Set aside some time each day or week to at least scan...titles of what’s going on in your research area. Set a goal for paper-reading, even starting out at 1 ...

  4. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...limitations of current methodologies. This section sets the stage for why your funded proposal will address...advisors Once a rough draft is in presentable form, set up a meeting with your advisor to go over format,... apply for funding improves the associated skill sets. Increased experience in communicating and motivating...detailed scope of specific aims, milestones, possible setbacks, and potential alternative routes, Provides ...

  5. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...The moderator (possibly with an organizer) should set up a 30 minute call with each panelist to get to ...remind them about the event as it approaches. Room set-up and logistics To make a really lively discussion...level as the audience. In my experience the best set-up is panelists in a slight semi-circle (so they ...

  6. Finding Your Science Policy Path

    Type
    Blog Post
    ... through the integration of science with a large set of other societal pressures including economic, cultural... typically think of. It occurs in a government setting: drafting legislation, making rules, and implementing...convening activities, formal lobbying, or strategy setting.  The “little p” policy is non-governmental work... the profession. One of the most important skill sets for a science policy career is science communication...

  7. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...multiplexing. pCri plasmids are divided into two sets, each of which includes plasmids with different ... varieties of tags, both N- and C-terminal. Each set of plasmids uses the same restriction enzymes for...

  9. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...longer-lasting effects. Building your chemogenetic system To set up a chemogenetic system, you’ll need to choose a...retain their intracellular interactions and still set off the same pathways, giving rise to the same cellular...cellular responses. The cellular response set off by a DREADD depends on what G-protein and, consequently...drugs that must diffuse throughout the body, the onset of receptor activation is delayed and takes on the...

  10. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...Controlling stimulation Invasiveness  Choosing the best set of tools therefore depends on what you are looking...with all tools, both approaches suffer from unique sets of off-target side-effects that should be carefully...the diffusion of drugs throughout the body, the onset of receptor activation takes minutes. As a result...can be injected, this is not as precise and the offset of stimulation is gradual. Invasiveness Both optogenetic...DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad...

  12. Advice for Choosing a Research Project

    Type
    Blog Post
    ...forward? Most projects typically require a diverse set of skills. It is important to know who in the lab...benchmarks. Think about what your personal goals are and set milestones along the way so that you don't get stuck...

  13. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...in yeast imaging, categorized by specific filter sets and experimental output requirements. Select from...GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in S. cerevisiae, with the additional...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...spectrophotometers have an IgG protein setting. If yours does not, be sure to set the absorbance to 280 nm (Pace... antibody is not IgG, make sure to look up the setting for your isotype.  Antibody concentration may also...

  15. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...high-fidelity” junction sets for both the 12- and 24-fragment versions of the lacI/lacZ cassette were designed ...- or 24-fragment test systems was performed by setting up assembly reactions in which a single component...sequences under standard reaction conditions. This data set allows quantitation of sequence-dependent ligation... the ability to correctly assemble a lacI/lacZ cassette (designed by NEB for use in optimization of assembly...sequence for beta-galactosidase in the lacI/lacZ cassette. Additional confirmation of accurate assemblies...12- and 24-fragment assemblies of the lacI/lacZ cassette, and illustrates how the volume of the 1 ml outgrowth...and assembly efficiency Five fragment lacI/lacZ cassette assembly was easily achievable with high levels...

  16. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...performance via the depletion of a gold standard set of genes (1,580 essential and 927 non-essential genes...off-target effects. The Dolcetto library contains two sets of sgRNAs; A, the top 3 ranked sgRNAs, and B, the...sgRNAs were selected per gene and divided into two sets, A and B. Sanson et al. demonstrated that the Calabrese...cutting. A Dolcetto library containing only 3 sgRNAs (setA) also outperformed CRISPRi libraries with 10 sgRNAs...

  17. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ... denotes intersection and the – operator denotes set difference. That’s the intuition for how genome math...1: Genome math illustrated. Intersect (left) and set minus (right). Enhancers are regions of DNA that...Intergenic CRISPRs Yours truly generated the DHS data set by starting with all of the DHS sites from 125 different...

  18. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...remove the plate seal from the 96-well plate and set aside. Aspirate the wells and use a multichannel ...the plate. Place the plate on a microplate shaker set at 400 rpm and shake for 1 min . Repeat steps 8–10...a plate seal and incubate on a microplate shaker set to 400 rpm for 2 h at room temperature or overnight...the plate Place the plate on a microplate shaker set at 400 rpm and shake for 1 min . Repeat steps 18–...

  20. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...applications. Recently, the Spiess lab developed a set of functionalized nanobodies to study retrograde ... from the cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP nanobody ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...Sontheimer and his group from the University of Massachusetts Medical School have developed a group of plasmids...
Showing: 81 - 100 of 618 results