We narrowed to 616 results for: Set;

...searching and reading a regular part of your routine. Set a goal to read a certain number of papers each week...up with the literature in your field of interest. Set aside some time each day or week to at least scan...titles of what’s going on in your research area. Set a goal for paper-reading, even starting out at 1 ...

...limitations of current methodologies. This section sets the stage for why your funded proposal will address...advisors Once a rough draft is in presentable form, set up a meeting with your advisor to go over format,... apply for funding improves the associated skill sets. Increased experience in communicating and motivating...detailed scope of specific aims, milestones, possible setbacks, and potential alternative routes, Provides ...

...The moderator (possibly with an organizer) should set up a 30 minute call with each panelist to get to ...remind them about the event as it approaches. Room set-up and logistics To make a really lively discussion...level as the audience. In my experience the best set-up is panelists in a slight semi-circle (so they ...

... through the integration of science with a large set of other societal pressures including economic, cultural... typically think of. It occurs in a government setting: drafting legislation, making rules, and implementing...convening activities, formal lobbying, or strategy setting.  The “little p” policy is non-governmental work... the profession. One of the most important skill sets for a science policy career is science communication...

...multiplexing. pCri plasmids are divided into two sets, each of which includes plasmids with different ... varieties of tags, both N- and C-terminal. Each set of plasmids uses the same restriction enzymes for...

...the names of everyone at Addgene, and send random sets of 4 people out to lunch.   I think these random...food, and these lunches would provide a friendly setting for people in different labs to chat informally...

...longer-lasting effects. Building your chemogenetic system To set up a chemogenetic system, you’ll need to choose a...retain their intracellular interactions and still set off the same pathways, giving rise to the same cellular...cellular responses. The cellular response set off by a DREADD depends on what G-protein and, consequently...drugs that must diffuse throughout the body, the onset of receptor activation is delayed and takes on the...

... fun every day.  With these three main values we set out to radically redesign our homepage to reflect...reinforcing the three values with each element. We also set out to make sure the page looked clean, professional...

...Controlling stimulation Invasiveness  Choosing the best set of tools therefore depends on what you are looking...with all tools, both approaches suffer from unique sets of off-target side-effects that should be carefully...the diffusion of drugs throughout the body, the onset of receptor activation takes minutes. As a result...can be injected, this is not as precise and the offset of stimulation is gradual. Invasiveness Both optogenetic...DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad...

...forward? Most projects typically require a diverse set of skills. It is important to know who in the lab...benchmarks. Think about what your personal goals are and set milestones along the way so that you don't get stuck...

...in yeast imaging, categorized by specific filter sets and experimental output requirements. Select from...GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in S. cerevisiae, with the additional...

...high-fidelity” junction sets for both the 12- and 24-fragment versions of the lacI/lacZ cassette were designed ...- or 24-fragment test systems was performed by setting up assembly reactions in which a single component...sequences under standard reaction conditions. This data set allows quantitation of sequence-dependent ligation... the ability to correctly assemble a lacI/lacZ cassette (designed by NEB for use in optimization of assembly...sequence for beta-galactosidase in the lacI/lacZ cassette. Additional confirmation of accurate assemblies...12- and 24-fragment assemblies of the lacI/lacZ cassette, and illustrates how the volume of the 1 ml outgrowth...and assembly efficiency Five fragment lacI/lacZ cassette assembly was easily achievable with high levels...

...performance via the depletion of a gold standard set of genes (1,580 essential and 927 non-essential genes...off-target effects. The Dolcetto library contains two sets of sgRNAs; A, the top 3 ranked sgRNAs, and B, the...sgRNAs were selected per gene and divided into two sets, A and B. Sanson et al. demonstrated that the Calabrese...cutting. A Dolcetto library containing only 3 sgRNAs (setA) also outperformed CRISPRi libraries with 10 sgRNAs...

...spectrophotometers have an IgG protein setting. If yours does not, be sure to set the absorbance to 280 nm (Pace... antibody is not IgG, make sure to look up the setting for your isotype.  Antibody concentration may also...

... denotes intersection and the – operator denotes set difference. That’s the intuition for how genome math...1: Genome math illustrated. Intersect (left) and set minus (right). Enhancers are regions of DNA that...Intergenic CRISPRs Yours truly generated the DHS data set by starting with all of the DHS sites from 125 different...

...transcriptional activation or repression. The BIOFAB plasmid set (Addgene #1000000037) contains specific bacterial... and Venus) Escherichia coli Wolf Frommer 20336 pRsetB-his7-Perceval ATP:ADP ratio Fluorescence (GFP) ...

...invincible. Bondy-Denomy et al. identified the first set of anti-CRISPR genes in bacteriophages infecting ...

...Targeted gene activation using SAM There are two sets of 3 vectors each available for mammalian endogenous...2013). We also have an older system using two cassettes to express the CRISPR RNA array and tracrRNA separately...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested.... SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SaCas9 driven by either...This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized SaCas9; SaCas9...

...remove the plate seal from the 96-well plate and set aside. Aspirate the wells and use a multichannel ...the plate. Place the plate on a microplate shaker set at 400 rpm and shake for 1 min . Repeat steps 8–10...a plate seal and incubate on a microplate shaker set to 400 rpm for 2 h at room temperature or overnight...the plate Place the plate on a microplate shaker set at 400 rpm and shake for 1 min . Repeat steps 18–...

... recombinant antibodies. The first collection, a set of integrin antibodies, will be available on Addgene...