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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 731 results for: Sis

Showing: 81 - 100 of 731 results

  1. AAV for Neuronal Tracing

    Type
    Collection
    ...is done using the envelope protein of (EnvA) of leukosis virus. EnvA can direct virus infection specifically...

  3. Antibody Production

    Type
    Collection
    ...applications such as these, the buffer can be removed by dialysis or gel filtration. Titer and Purity Antibodies...

  4. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species...

  5. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species...

  8. AAV Packaged on Request

    Type
    Collection
    ...answer isn’t in the Help Center or you need more assistance with troubleshooting, we want to hear from you...

  9. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species...

  10. TALEN Guide

    Type
    Collection
    ...available as a kit from Addgene , custom arrays consisting of 12-31 repeats can be assembled and inserted...

  12. CRISPR Plasmids - Plants

    Type
    Collection
    ...U6 BbsI none S. pyogenes Gao 59188 pBlu/gRNA Arabidopsis U6 BbsI none S. pyogenes Stupar 62200 pBUE411...

  13. Plan Your Experiment

    Type
    Collection
    ...identify DNA fragments. PCR amplification and gel electrophoresis (for HDR or NHEJ): For large deletions or insertions...

  16. Genetic Code Expansion

    Type
    Collection
    ...sufficient levels of Sep in the cytoplasm for protein synthesis. Also see Strain 192872. Jesse Rinehart 69493 ...

  17. CRISPR Antimicrobials

    Type
    Blog Post
    ...without a resistance gene, allowing resistant bacteria to multiply, spreading their resistance genes throughout...The crisis of antibiotic resistance is upon us, and the world is unprepared. Each year in the United ...antibiotic resistant bacteria. Even when researchers develop new antibiotics, the onset of resistance is swift...antibiotic resistant bacteria with a weapon that they’ll have less potential to become resistant to? CRISPR...antibiotic resistance Most antibiotics target one of three essential processes: cell wall synthesis, DNA replication...or antibiotic resistant bacteria, and inappropriate antibiotic usage selects for resistant bacteria, allowing...propagate their resistance genes. Our society is hoping to combat antibiotic resistance using multiple ...

  18. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...example, we examined ICE analysis of Sanger sequencing data alongside the analysis of NGS-based amplicon ...scalability, the ICE software has two analysis formats: sample by sample analysis, which can compare up to five...synthego.com. Overview of ICE editing analysis Once the analysis is complete, a new screen appears with...also download the entire analysis as a ZIP file by clicking the Download Analysis Data button on the bottom...bottom right of the analysis screen. ICE analysis details Contributions   The Contributions tab shows the...tool was initially created to support the CRISPR analysis needs of Synthego’s scientists, and it’s now free...sequencing data and found that the accuracy of ICE analysis results was highly comparable (within r2=0.96)...

  19. Deep Dive: qPCR

    Type
    Blog Post
    ...   Statistical analysis  Getting your RQ value isn’t the end of your analysis process. You’ll still...different statistical analysis methods that pair well with slightly modified CT analysis, so pick one and ...chemistries, primer optimization, controls, and analysis.  As a rule of thumb, you can assume that qPCR...chemistry  The two most common qPCR chemistries are hydrolysis probes (which you probably know as TaqMan) and...multiplexed Single amplicon validation technique Hydrolysis Probes (Taqman) Low; can be reduced with a ... Yes, one Yes Yes Agarose gel or uMelt analysis dsDNA dye (Sybr)  High No No No ... Yes, two Yes Yes Agarose gel or uMelt analysis Hairpin probes Low Yes, one Yes Yes...

  20. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...cancer development, metastasis, and drug resistance, Bhang says “the [drug] resistance story panned out ...cells that represent the preexisting resistant subpopulation 2. Resistant cells among the separate replicates...found that similar barcodes consistently made up the majority of the resistant populations. In fact, 88%...apply [it to].” Using ClonTracer to study drug resistance Although Bhang and co-workers started using the...determine whether or not cancer cell populations resistant to drug treatments arise during treatment or are... outcomes (see schematic depiction below): 1. Resistant cells among the separate replicates would contain...abundance before and after a treatment. The library consists of a pool of lentiviral vectors with roughly 73...
Showing: 81 - 100 of 731 results