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...sales training and knew I had to start somewhere. I knew if I started with the Harvard Business Review...and cloning, I decided to pack it in. I had just started month 8 of my 3 year Post-Doctoral contract at ...interview. The simplest place I could think of to start was “Marketing for Dummies”. The fastest way to ...to learn something about marketing would be to start reading a broad simple overview, learn some jargon...academia, sales and marketing can be great places to start. Depending on what type of career you would like...

...easily started and easily credited. And nothing stops these one-time favors from becoming starting points... rewarding way of making science.   How we started Crowdfight-COVID19 Crowdfight was born as a collaborative... all over the world had signed up, and soon we started receiving dozens of requests every day.    ...suitability and availability; 3) Make the contact to start a direct collaboration between researcher and expert...are undergraduates or PhD students who are just starting their journey into science. Others are experienced...

... the internet, but start by downloading my handy list of suggestions.  Get started. Have regular meetings...elusive teachers, supporters and advisors? I tried to start a more formal mentoring program at my company, but...mentoring relationships.   If you would like to start a group mentoring program at your organization, ...provide a step-by-step, how-to guide to get one started. Advantages of the group mentoring format Adding... speaking to grad students and postdocs I have started to talk to trainees more about peer mentoring. ...

...because it has the advantage of starting out with a larger initial starting library size (up to 1012 members...To find an optimally binding sybody, you want to start out with a large and diverse pool of sybodies that...al., 2018). Find the Sybody Generation Toolbox! Starting with libraries and a biotinylated (for purification...et al., 2018. Ribosome display The Seeger lab starts with a “pre-enrichment” selection step using ribosome...Zimmermann et al., 2020). This step of the protocol starts out with anti-Myc antibodies bound to an ELISA ...

...often called “nontraditional,” helped prepare me to start CINSO. Here, I share some of the tips I learned ... had to take advantage of it. Blog and tweet I started my blog, Born to Science, as a graduate student...experience with my blog helped immensely when CINSO started brainstorming the resources we wanted to provide...my career path began to take shape, was when I started volunteering. I wasn’t sure yet what a career in...I volunteered at a place that made me happy. I started volunteering in the “Dinosphere” almost every week...the dinosaurs, and met wonderful people. When I started volunteering, I was worried about becoming one ...

...genome as a map with three coordinates: chromosome, start, and stop. For instance (chr3, 1, 10) indicates ...find all coffee shops near your house, excluding Starbucks. Taking a nerdy perspective, you might denote ...your search as follows: (my_house + coffee) - starbucks See how the notation works? The + operator denotes...above formula to a real-world experiment. We’ll start with ChIP-seq data from the ENCODE project, find... final step is to conduct our CRISPR screen. We start by infecting millions of embryonic stem cells (ESCs...CRISPRs Yours truly generated the DHS data set by starting with all of the DHS sites from 125 different human...

...creative problem-solving process?  You may want to start by carving out time to tackle it. It’s easy to get...Another strategy for your brainstorm session is to start with something completely unrelated to the issue...were instructed to use the three-ring binder as a starting point, the ideas generated were hardly novel. .... When told to use a roller skate as a starting point, their ideas scored 37% higher in originality. It...behind the study holds. To generate novel ideas, start with an out-of-the-box concept and pare it down ... the best it can be that it seems impossible to start at all. This is why perfectionists tend to also ...

.... I started watching the questions and preparing answers early in the day. Although I didn’t start answering...description of our AMA and Joanne’s preferred time to start answering questions. r/Science has pretty strict...was posted at 8:00 am and Joanne didn’t have to start answering until 5 pm). The ability to see the questions...Fortunately, Reddit Science is heavily moderated. I started watching the questions and comments go up early...things an early career scientist can do is to get started using LinkedIn early and brush up on best practices...

...was using.  I started to do little things to save reagents and money.  This was the start of my interest... a popular topic in the media.  At one point I started to become very aware of how little care I took ...time and money. My other stingy science project started when I realized I was pouring large agarose gels... a small portion of the gel.  What a waste?  I started looking for smaller and smaller gel apparatus until...

...and so far most of their funding has come from kickstarter and fundrazr projects). This allows the project... to a wider group of people. This is a perfect starter project for our computer scientists, many of whom...interested in biology but don’t really know how to start applying their skills to it. The Kittybiome project...Funding Makerspaces One of the main challenges when starting a new project at a makerspace is finding the financial...find a makerspace somewhere near you. The place to start looking for your local space is the Hackerspace ...

... For anyone just starting to use flow cytometry, it’s generally recommended to start compensating with...needing to use your single-color controls as a starting point. Applying compensation to your experimental...your main population and singlets. Then, you can start to compensate on your experimental samples. Let'...challenging! But we hope this guide can help you get started. Remember, you can always change your compensation...

...they are useful, how they work, and how to get started with them. Why Use multicistronic vectors? Detecting...added to the end, while the downstream cistron will start with the Proline.  The table below lists the four...peptide to improve cleavage efficiency. How do I get started? If you are looking to co-express your gene of ... protein or selectable marker, it is easiest to start with a plasmid that already has the multicistronic...co-expression of multiple unique genes, you can start with a plasmid that has multiple cloning sites flanking...

... retinal gene therapy. The year was 2001 and I started a job as a technician in a lab using adeno-associated...hemophilia, muscular dystrophy)? AAV gene therapy started in the mid-90's with a lot of work and a little... luck A pioneer in the field, Dr. Jean Bennett started working on ways to deliver genes to correct genetic... another 6 years before the 1st clinical trial started, and 10 more years to obtain FDA approval and make... first clinical study combining these tools may start as early as next year. Twenty years after falling...

...producing and purifying it in your lab. Instead of starting a new search or clicking through to a different...Materials or elsewhere in their papers. Maybe they started their deposit prior to publication and were able...searching with filters Not every plasmid search will start from a publication. You may have an idea for a plasmid...express ubiquitin under control of a CMV promoter. Starting with two search terms we see there are plenty ...need to apply different filters you do not need to start a new search. Simply click the ‘X’ next to any filter...

...kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL bacterial culture Product: plasmid... DNA gel extraction with glass syringe filters Starting material: bacterial cultures or agarose gel slices...commercial kits.  DNA gel extraction with glass milk Starting material: agarose gel slices Product: DNA fragments... enzymes.  Cellulose nucleic acid purification Starting material: plant, microbe, blood, or mammalian ...Regeneration of silica columns for DNA purification Starting material: PCR product or agarose gel slices Product...

...can have when starting in an academic lab. I floundered for a solid year after I started my postdoc! ...cloning and lentivirus production, I didn’t really start to recognize my transferable (or “soft”) skills ...you’re out of the lab to think about these--get started with our Transferable Skills Guide.     Over ...gloves again! The opportunities are there once you start looking for them. Acknowledgements Big thank you...

...suspenseful. 4. Start preparing your talk early - like today Don’t wait until you need to speak to start creating...’s attention will start to wander when you are supposed to be done. They will start looking at their phones...future boss in the room, so it is a good idea to start thinking about public speaking early and often. ...

...field is simply to know where to look and how to start. There is value, then, in having a guide that curates..., podcasts, and communities to help people get started in synthetic biology. While not everything is open-access...various applications. I think these papers are good starting points to understand the contours of each topic...many people will find this guide useful as they start their synthetic biology journey. And if you have...

...just dive right in and start injecting your virus, a successful AAV experiment starts with validation and...your system that can affect expression, before starting the selection process. Factors to think about ...expression.   Once you know that, you’re ready to start selecting, testing, and optimizing three key variables...in your system and then test each vector before starting your experiments (Rolland et al., 2016; Su et ...

...step. As with standard IF, you will need to start with a fixed sample. Depending on the sample type...photobleaching or by stripping out the antibodies, then start over with the next target: add the new primary, ...Abcam's sample protocol is a good place to get started.  One important consideration when deciding whether...not being degraded. Whether you’re just getting started with two or three targets or you’re pushing the...variations you could try. We hope this post helped you start planning for your next multiplex IF experiment! ...