We narrowed to 614 results for: TIL;

...Easy-to-use tools to monitor these features were lacking until Roberta Gottlieb and Zhen Yan labs developed MitoTimer...constructed using Golden GATEway cloning have been utilized to create recombination template vectors, to perform...cloning vectors (Level 0, 1, or 2) which can be utilized in three successive assembly steps. Before beginning... (SSA) assay. This new Platinum Gate TALEN Kit utilizes a 4-module assembly system, which reduces the ... of synapses in the mouse brain. Kim et al is utilizing a mammalian GRASP (GFP reconstitution across synaptic... were reported in 2007. In order to extend the utility of the system, the researchers developed three ...plasmid kit page or read our blog post: Quick, Versatile Plant Transgenesis with GreenGate Plasmids. ...

...visualization techniques. In addition, their incredible versatility opens aptamers for use in many entirely new applications...signal-off” biosensors in which the aptamers fluoresce until they bind to the appropriate ligand, but relying...the bound protein fluorophores and quenchers are still relatively bulky. One of the advantages of oligonucleotides...stable and brighter version of the aptamer, but it still had limitations in vivo. Using a combination of ... a lower magnesium dependence. Though initially still unstable in eukaryotes, the Jaffrey lab has since...

...experiments. There are far too many behaviors that have utilized optogenetics to be fully summarized in a short...circuit is sufficient to induce reinforcement (instills a sense of “reward”), then the animal will repeatedly...type of behavior, certain technical considerations still apply. Testing stimulation First, In lieu of electrophysiology...prove inhibition of neuronal activation, but it may still be possible to use c-fos expression to get the evidence...advances of some are shared with all. Due to the versatility and standardization of genetic tools in the laboratory...

...plasmid replaces the chromosomal copy. This is a versatile technique, and is less prone to off-target mutations...counter-selection system relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is...that have only gone through the single cross over still contain sacB and will not grow on sucrose. One ...et al., 2014). Replaced the pDS132 MCS with a versatile, synthetic MCS (designed with the help of the ...and continue to improve the efficiency of this versatile technique. Many thanks to our guest blogger Jacob...

...or cell culture. For example, Scott Lowe’s lab utilized mice to study how different mutations in leukemia...at a time. Zebrafish embryos are also laid and fertilized externally, allowing scientists to easily manipulate...sizes. The majority of C. elegans are also self-fertile hermaphrodites making it easy to conduct large ...to name a few. For example, Andrew Leifer’s lab utilized the worm to study calcium signaling in the entire...PMC3703927. Jennings, Barbara H. "Drosophila–a versatile model in biology & medicine." Materials today ...

...nucleotide transitions but still requires a nearby PAM, although these are still early days for this technology...gRNAs to 5 – 65% of the protein coding region will still result in many dozens of gRNAs to choose from. With... While SpCas9, with a PAM preference of NGG, is still the most widely-used Cas enzyme, the development...basic landscape of mismatches that can nevertheless still lead to activity has been established, and can be...

...other cellular proteins. BRET-based biosensors utilizing NanoLuc® luciferase Many intracellular sensors... activation of other fluorescent probes but can still transfer energy to the fluorescent protein acceptor...researchers to convert FRET biosensors into more versatile BRET biosensors that can even be paired with optogenetic... from Addgene and we're excited to see how you utilize them in your group! Many thanks to our guest ...Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem ...

...spectrum and emission spectrum. Fluorescent dyes utilize these fluorophores by combining them with a molecular...protein tags, such as high specificity, increased versatility in probe attachment, and compatibility with far-red...These variations directly impact the way you can utilize these dyes. The most-used fluorescent dye is fluorescein...are currently offered at Promega. Due to the versatility of interchangeable labeling using the HaloTag...driven by the need for more precise, stable, and versatile imaging tools in biological research. This does...

...Tags. Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids...than E. coli, specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory.... pCri vector pCri-13a, designed for use in B. subtilis, contains the MISTIC (membrane-integrating sequence...proteins that natively localize there. pCri is both versatile and easy to use, and we hope that it will take...

...Addgene depositor Indu Maiti has created a new and versatile binary Ti vector for both transient and stable...protein expression via Western blotting. Given its versatility and customizability, pSiM24 can be used not only...Dey, and Indu Bhushan Maiti. "pSiM24 is a novel versatile gene expression vector for transient assays as... Challenges in Plant Synthetic Biology Quick, Versatile Plant Transgenesis with GreenGate Plasmids Interview...

...valuable laboratory tool are still an area of active research. New details are still being uncovered, and different...This helps explain why Cas9 can’t locate PAM sites until after RNA loading: the structure to interrogate ...produce SuperFi-Cas9, a high-fidelity variant that still retains high cleavage efficiency (Bravo et al., ...et al., 2014). New discoveries on this topic are still being made! Conclusion Finally, we can summarize...

...flexibility, versatility, safety, and cost-effectiveness enable molecular biologists to broadly utilize plasmids...antibiotic resistance gene. In contrast, plasmids utilized in the lab are usually artificial and designed...gene of interest into a vector, scientists may utilize one of a variety of cloning methods (restriction...

...quantify Cas9 cleavage, thus exhibiting its versatile utility in quantifying multiple gene editing activities...2018. PubMed: PMID 30220597. ACE system: A versatile in vivo fluorescent reporter for rapid quantification...fluorescence-based quantification of base editors that utilize dCas9 fused to cytidine deaminases like APOBEC....

...Science is failing over and over and over again until you don’t.” As every researcher can attest to, science... that the superheroes win in the end. But we’re still invested in seeing how they do it.    Finding...within that process of failing, over and over again until you don't, of trying to understand your completely... said that even when we know the ending, we can still get invested in the journey? The same thing applies...

... not naturally exist in mammals, scientists can still reap the benefits of plasmid-based research using... a reporter and we will be covering this ever-versatile fluorophore in a later post, so stay tuned! Transient...that you may not want the plasmid depleted, but still want to use transient transfection methods. Since...selection agent should not be added to culture media until 24-48 h post transfection when creating stable cell...

...chemically modified to resist degradation while still effectively serving their function of guiding Cas9...The photoactivatable counterpart to this system utilizes a gRNA which is photocaged and unable to engage...initiate targeted editing events. Both technologies utilize chemical modifications to the RNA to control the...molecule) you will not have that option, but you can still choose what dye to use  What to do once you’ve chosen...

...focus of the application was changed, but Sample6 still leverages the core engineering and scientific concepts...into the local academic community. However, Mike still could not remove himself from the idea of starting... importantly, “the company does not become real until someone takes the risk full-time and abandons all... many universities throw out old equipment that still works. “We use functional spectrometers from the...

.../Cas9 effector (LACE) system, the Gersbach lab utilized a similar strategy to develop an optimized photoactivatable...repair (HDR). This system is unique in that it utilizes a “split” nuclease - the authors fragmented Cas9...the figure below. Their most successful design utilized Magnet photoswitchable proteins derived from the.... aureus Cas9 (saCas9) proteins, showing the versatility of this approach. This study generated the first...formed 20-40 min later. The CASANOVA system is versatile in that it can be adapted to regulate gene editing...

...experiments in S phase zygotes at 18 hr post fertilization. They observed higher targeting efficiency than...41/42 embryos were non-mosaic at 3 days post fertilization. Since Ma et al. designed a repair template ...recessive gene. Ma et al. claim that CRISPR would still be helpful in increasing viable embryos, especially... in terms of editing success rate. Would CRISPR still be worthwhile for a given disease if it increased...

... that the majority of attendees were scientists still working at the bench. It was a fantastic opportunity...unintended themes of the meeting was how much we still don’t know about RNA. One talk that really brought...about his work on the 500+ human tRNA genes and, until now, we've overlooked regulatory fragments of these... summed up this conference—nucleic acid science still has many surprises for the researchers in this field...