We narrowed to 152 results for: Tore

1. All in a Twist: dsRNA

   Type

   Blog Post

   ... some exist as dsRNA. Still others use dsDNA to store their genetic information. All of these viruses ...

2. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   ...are sequencing from the exact same stock that we store and distribute from. Of course, your culture should...

3. Bacterial Expression Systems

   Type

   Collection

   ...Strains Why Bacteria? E. coli are commonly used to store and replicate plasmids of all types, but beyond ...

4. Tetracycline Inducible Expression

   Type

   Collection

   ...cell in a population. Proliferation history is monitored by the dilution of a dox-inducible H2B-mCherry...

5. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   ...significantly higher than the same prep that has been stored in the freezer. If you plan to freeze a batch of...

6. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

   Type

   Blog Post

   ...excited to see what the next few months have in store for us. If you’re thinking about making the switch...

7. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   ...-3.13. To test xCas9 3.0-3.13, Hu et al. first restored catalytic residues D10 and H840. To ensure that...

8. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   ...preparation.  rAAVs  Now your AAV virions are ready to store or use. They are quite stable and will withstand...

9. Viral Vectors 101: Voltage Indicators

   Type

   Blog Post

   ... linked to a chromophore and functions as a photoreceptor in the eye. There are two flavors of rhodopsin...

10. Anatomy of a Plasmid Page at Addgene

    Type

    Blog Post

    ...survival (for Gateway plasmids).  We also like to store plasmids that need extra stability, such as some...

11. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    ...testing. An aliquot of our recombinant antibodies is stored at 37 °C for a period of two weeks. After this ...

12. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    ...titrating AAV by qPCR, viral DNA is amplified and monitored in real time. When the PCR is complete, the results...

13. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    ...containing buffer, polymerase and dNTPs, can be stored frozen: just defrost and add your primers and template...

14. Plasmids 101: Colony PCR

    Type

    Blog Post

    ...cultures of your positive clones. If you want to store your clones longer term, just streak the colonies...

15. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    ...caused by stress and fear. Such a behavior can be monitored in an open field test (Alpar et al., 2018). Locomotion...

16. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    ... response to an experimental treatment can be monitored. This can reveal previously unknown functions ...

17. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type

    Blog Post

    ...Discovering ways to culture, propagate, transform, store, and share all of these new organisms continues ...

18. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    ... this mutation by an adenine base editor would restore antibiotic resistance. To find such a protein, ...

19. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    ...precise amount of protein to each brain cell to restore physiological levels. Much research is now focused...

20. How to Be an Excellent Trainee

    Type

    Blog Post

    ... go ahead and write down where that one drug is stored or how to access the shared server. The blue box...