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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 244 results for: Xpa

Showing: 81 - 100 of 244 results

  1. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...., et al.,Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science 361,1259-1262(2018). ... on the Addgene blog The PAM Requirement and Expanding Beyond Cas9 xCas9: Engineering a CRISPR Variant...

  3. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...AAV1! New INTRSECT viral preps available! We are expanding our inventory of ready to use viral preps from...imaging calcium dynamics in fine structures. We have expanded our recombinase-dependent virus selection to now...

  4. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution Patterns of Diverse..., S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution Patterns of Diverse...

  6. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...., Grames, M. S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector...with Addgene’s AAV Data Hub!Data Freedom: The Expansion of Data Sharing in Research Publications...

  7. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...through the development of tools that exploit and expand our current knowledge. The Abudayyeh-Gootenberg... Lately, they’ve been working on two tools that expand and exploit CRISPR, both of which we're featuring...

  8. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...then read on for your best options. There are an expanding number of Cas9s with looser PAM requirements compared...Cas9s can be found in “The PAM Requirement and Expanding CRISPR Beyond SpCas9.” Single base pair edits ...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...C2c2), has several unique properties that further expand the CRISPR toolbox. We'll cover how Cas13a was ...diversity to the CRISPR toolbox and will continue to expand the applications of CRISPR. Since this post was...

  11. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...video to complement traditional print journals and expand our ability as researchers to reach new audiences...Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources...

  12. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...alternatives to GFP is expected to grow with the ever-expanding genetic toolbox generated by the continuous effort...PubMed PMID: 28401765. 15. Takai, Akira, et al. "Expanded palette of Nano-lanterns for real-time multicolor...

  13. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... offers additional PAM options that can greatly expand gRNA options. Created with BioRender.com...exciting time for functional genomics, with an ever-expanding list of tools to probe gene function. The best...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...sequence homology (usually 30-40bp overlaps). To expand the capabilities of Gibson assembly and make a ...scientists use GMAP, there will be a continuous expansion of compatible backbones, promoters, and genes ...depositing your new plasmids with Addgene to help expand the collection! The Jacks lab has made several ...

  18. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...Single cells from a stable cell pool were then expanded for an additional 3 weeks under blasticidin selection...stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type A549 cells do not...

  19. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...CRISPR CRISPR-Cas can harness the power of MoClo to expand both targetability and effectiveness. Perhaps the...tools, it should be easy enough for researchers to expand the Fragmid toolkit to facilitate other lentivirus...
Showing: 81 - 100 of 244 results