We narrowed to 241 results for: Xpa
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TypeBlog Post...interview and Expand your professional network Volunteer at a professional organization to expand your skill...
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Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution Patterns of Diverse..., S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution Patterns of Diverse... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...for lists of alternatives to BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post...., Grames, M. S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector...with Addgene’s AAV Data Hub!Data Freedom: The Expansion of Data Sharing in Research Publications... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog Post...different TAM (5′-TTAT-3′). The unique TAM requirements expand the number of genomic sites that can be targeted...continues to evolve, we anticipate its use will expand into diverse plant and animal systems, potentially... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...then read on for your best options. There are an expanding number of Cas9s with looser PAM requirements compared...Cas9s can be found in “The PAM Requirement and Expanding CRISPR Beyond SpCas9.” Single base pair edits ... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...through the development of tools that exploit and expand our current knowledge. The Abudayyeh-Gootenberg... Lately, they’ve been working on two tools that expand and exploit CRISPR, both of which we're featuring... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...C2c2), has several unique properties that further expand the CRISPR toolbox. We'll cover how Cas13a was ...diversity to the CRISPR toolbox and will continue to expand the applications of CRISPR. Since this post was... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...video to complement traditional print journals and expand our ability as researchers to reach new audiences...Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...alternatives to GFP is expected to grow with the ever-expanding genetic toolbox generated by the continuous effort...PubMed PMID: 28401765. 15. Takai, Akira, et al. "Expanded palette of Nano-lanterns for real-time multicolor... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post... offers additional PAM options that can greatly expand gRNA options. Created with BioRender.com...exciting time for functional genomics, with an ever-expanding list of tools to probe gene function. The best... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...after your training. You must ensure that you are expanding your options and preparing for both academic and...conflicts PI has no idea who is dating whom Expanding your skillset Scientists are lifelong learners... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...presentations, and preparing for job interviews, expanded profesional networks, and nominations for government...possible a significant qualitative and quantitative expansion in the 2015-2016 academic year. This year, the... -
27 Hot Plasmids from 2016
TypeBlog Post...sequence homology (usually 30-40bp overlaps). To expand the capabilities of Gibson assembly and make a ...scientists use GMAP, there will be a continuous expansion of compatible backbones, promoters, and genes ...depositing your new plasmids with Addgene to help expand the collection! The Jacks lab has made several ... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...cle10ts) allele), increasing experimental options. Expanding and improving the CRISPR/Cas editing toolbox for...need to segregate away co-conversion markers. Expanding beyond Cas9 Since the development of CRISPR/Cas9... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post...Single cells from a stable cell pool were then expanded for an additional 3 weeks under blasticidin selection...stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type A549 cells do not... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic Code Expansion Collection... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...CRISPR CRISPR-Cas can harness the power of MoClo to expand both targetability and effectiveness. Perhaps the...tools, it should be easy enough for researchers to expand the Fragmid toolkit to facilitate other lentivirus... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering ...protein function and signaling networks continues to expand. The labs of Jesse Rinehart and Dieter Söll at ... -
Suggest A Plasmid You'd Like to Find at Addgene
TypeBlog Post...will end up depositing, we hope this campaign will expand the repository in ways that will be most useful...