...For example, hematopoietic stem cells found in the bone marrow only differentiate into cells of peripheral... 19343304. PubMed Central PMCID: PMC2752493. 4. Bongso, A. and M. Richards, History and perspective of...

... based on the copy number of the empty plasmid backbone or a researcher’s personal observations. If you..., buffering agents, and/or supplying additional carbon sources such as glycerol or glucose (in moderation...

...generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro...Topic Page Learn about CRISPR Delivery Using Ribonucleoproteins (RNPs) Read Tips for a 1st Time CRISPR User...

...making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element, leading to separation...insert the whole multicistronic cassette into a backbone as a single unit. Although it is recommended to...

...expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm. Microbial Cell Factories...-White Screening Choosing Your Perfect Plasmid Backbone  ...

...plasmid backbone that was designed specifically for transient expression (find some useful backbones here...

...protocol!  First, the authors created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...

...an option to transduce cells directly with ribonucleoprotein cargo. eVLPs contain no genetic material, ...particles for transient delivery of prime editor ribonucleoprotein complexes in vivo. Nature Biotechnology, 10.1038...

...interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags to your gene,...metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl malate dehydrogenase ...

...plays some role, and mismatches with more hydrogen bonds are more readily ligated than those with few. For...many ligases, G:T mismatches, with two hydrogen bonds and a base-pair size nearly indistinguishable from...

...uses the direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates...//doi.org/10.1093/nar/gku289 Chen C, Fenk LA, de Bono M (2013) Efficient genome editing in Caenorhabditis...

... show wide scale mobility with movement from the bone marrow and other lymphoid organs to specific tissues... to decipher the journey of lymphocytes from the bone marrow to the thymus where they gain their specificity...

...for the target:probe/primer bonds to be more stable than the hairpin bond.  Alternatively, chemistries...

...domain that promotes the formation of an isopeptide bond between 2 peptide tags, SpyTag and KTag. The group...plasmids for a given species are based on the same backbone to permit expression levels to be directly compared...

...Often, the size of the plasmid insert and vector backbone are known and thus this technique can be quickly...buffer Verifying Total Plasmid Size -OR- Insert and Backbone Size The simplest form of diagnostic digest is...the expected size, or that it is composed of a backbone and insert of expected sizes. This is frequently...in two bands; the 1200bp insert and the 5000bp backbone. Verifying Plasmid Identity by "Plasmid Fingerprinting...know that you have a 1,200bp insert in a 5,000bp backbone, but there are many plasmids out there that, when...very unique pattern that will distinguish one 5kb backbone with a 1.2kb insert from all others. When choosing...orientation the insert is ligated into the vector backbone and therefore the only thing left to do is identify...

...Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope protein for producing lentiviral and...

... over to our YouTube channel and watch it now! (Bonus: Tim kindly offered to finishing answering all submitted...

...winners dressed up as Addgene's favorite lunch... a BonMe noodle meal, crammed right into the box. Which is...

...full list here. Most of these plasmids are empty backbones, some of them containing fluorescent markers or...