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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 312 results for: bon

Showing: 81 - 100 of 312 results

  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...Thankfully, this is a hot topic with many resources! In Bondeson et al, they review all the tags discussed here...informed choice! References and Resources References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al....

  5. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... based on the copy number of the empty plasmid backbone or a researcher’s personal observations. If you..., buffering agents, and/or supplying additional carbon sources such as glycerol or glucose (in moderation...

  7. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element, leading to separation...insert the whole multicistronic cassette into a backbone as a single unit. Although it is recommended to...

  10. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...

  11. Hot Plasmids - August 2020

    Type
    Blog Post
    ...protocol!  First, the authors created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

  12. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...an option to transduce cells directly with ribonucleoprotein cargo. eVLPs contain no genetic material, ...particles for transient delivery of prime editor ribonucleoprotein complexes in vivo. Nature Biotechnology, 10.1038...

  13. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags to your gene,...metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl malate dehydrogenase ...

  17. Deep Dive: qPCR

    Type
    Blog Post
    ...for the target:probe/primer bonds to be more stable than the hairpin bond.  Alternatively, chemistries...

  18. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...domain that promotes the formation of an isopeptide bond between 2 peptide tags, SpyTag and KTag. The group...plasmids for a given species are based on the same backbone to permit expression levels to be directly compared...

  19. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...Often, the size of the plasmid insert and vector backbone are known and thus this technique can be quickly...buffer Verifying Total Plasmid Size -OR- Insert and Backbone Size The simplest form of diagnostic digest is...the expected size, or that it is composed of a backbone and insert of expected sizes. This is frequently...in two bands; the 1200bp insert and the 5000bp backbone. Verifying Plasmid Identity by "Plasmid Fingerprinting...know that you have a 1,200bp insert in a 5,000bp backbone, but there are many plasmids out there that, when...very unique pattern that will distinguish one 5kb backbone with a 1.2kb insert from all others. When choosing...orientation the insert is ligated into the vector backbone and therefore the only thing left to do is identify...
Showing: 81 - 100 of 312 results