AAV Q&A with Tim Miles
Type
Blog Post
... engineered AAV are presently built on an AAV9 backbone and so will very likely cross-react with each ...
We narrowed to 200 results for: ckb
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A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein Tags ... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post...immediately outside of the ITRs in the plasmid backbone and do not properly assemble, but these sequences... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post... a version that fits your setup: Plasmid Backbone Promoter pRSET OCaMP pRSET T7 pAAV-hSYN1... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...different wavelengths. Addgene provides empty backbones with the luciferase gene, a variety of expression... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...communication skillsClear communication is the backbone of effective management. Miscommunication often... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...Biology Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research ... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...lentivirus. Also, note that due to the size of the backbone, transformations require electroporation. References... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...try to use an excess of insert compared to the backbone plasmid; a starting point could be a molar ratio... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...toolkit upon the Yeast Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog Post...promoters were also tested in different plasmid backbones that varied in size or carried different genes... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...photoactivatable fluorescent proteins in empty backbones in the Michael Davidson Collection Find Photoswitchable... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular... -
CRISPRainbow and Genome Visualization
TypeBlog Post...Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags ... -
Developing Lab Management Software for Biology
TypeBlog Post...plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you simply don't have enough context... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12 (Figure 2). Cloning from the pAWp28 storage... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post...disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations N497A, ... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post.... https://doi.org/10.1038/518027a Freedman LP, Cockburn IM, Simcoe TS (2015) The Economics of Reproducibility...