... Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids. Selected...type-specific promoters or Cre lines. Spatial transcriptomics suggests that AAV-PHP.eB, when paired with ...target gene expression (EGFP, in the depositing manuscript) across the brain. AAV-BI-28 has been tested ...when using a ubiquitous CAG promoter. Spatial transcriptomics shows that its tropism is relatively more biased...Characterization of AAV Tropism and AAV-Mediated Transcriptional Changes via Single-Cell RNA Sequencing. Frontiers...Wang, A. Z., & Gradinaru, V. (2023). Spatial transcriptomics for profiling the tropism of viral vectors ...

...epitope-tagging experiments. Notably, we could tag >60 transcription factors in a short (3-week) time window; this...epitope tagging experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene(s) from Ensembl .../index.html Design crRNA/ssODN: Using Ensembl Transcript ID(s) as an input query, pick crRNA (guide RNA...strand (strand used by RNA polymerase II for transcription) are more effective for genome-editing events...

...community is generating a massive amount of descriptive transcriptomics data. Custom CRISPR screens designed ...pathway with many receptors, signaling molecules, transcription factors, and ligands. The presenter then showed...videos and text on the website for more detailed descriptions of how to use the software. Here is a brief ...

... fluorescent proteins, fusion proteins, or transcriptional regulators. In this system, Cas9 generates ... Find the CasX plasmids Drug inducible transcriptional activation and genome editing Yu Wang's lab...developed drug inducible CRISPR/Cas9 systems for transcriptional activation and genome editing by fusing Cas9...

...submission of a job application. 3) Read job descriptions carefully Be open minded and be bold. You don...qualifications to apply for a position.  A job description is usually a wish list and the hiring manager...skills and experience. Most Addgene PhD level job descriptions clearly describe that the roles do not involve...

...integrate within an exon of a gene to generate a transcriptional fusion. This may disrupt the endogenous function...spliced into the gene product resulting in a transcriptional fusion, much like promoter traps. While this...likely disrupt the gene it monitors due to transcriptional fusion.   Examples of promoter, enhancer...

...a job search website like Indeed. Do the job descriptions sound interesting? Is the work something you... types of jobs people with master's have Job descriptions often fail to capture all of the responsibilities...to present your work at conferences, help with manuscript and grant preparation, and lead your own research...

...to construct the beginning of my review - "The manuscript by ZZZ et al., describes ... The authors share... ask that data and resources described in the manuscript are accessible to the community, increasing the...recognize the hard work that the authors put in the manuscript. As a non-native English speaker, I sometimes...

...reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and Hnf4α, a process that like...reprogramming (CellTagMEF), at the end of the three-day transcription factor reprogramming treatment (CellTagD3), ...determined by at least day 13. However, distinct transcriptional states were seen as early as day 6. All together...

... lacks context unless it links to a verified description of that reagent online. Furthermore, if the link... link between the catalog number and reagent description is lost (e.g. a supplier is bought out by another...specific information about the plasmid: name, description, how the plasmid was generated, validated sequence...

...analysis scripts available, some associated with specific libraries, but any analysis script should return...automated manner. Addgene commonly uses a custom script derived from the count_spacers.py file from Feng...

...times have you looked at a diagram depicting transcription, or DNA repair, or replication, or any number...researching enhancer elements that control the transcription of a gene a megabase away, or looking at the... protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous...

...fusion to a receptor-transcription factor fusion. TEV protease cleave the transcription factor from the receptor...parallel receptorome expression and screening via transcriptional output - TANGO) (Kroeze et al., 2015). PRESTO-TANGO...

..., the ability to detect mRNA transcripts of toxins and other transcripts of interest is a benefit of this...the organisms present while sequencing of all transcripts gives clues into what the microbes are doing....

...includes overview information, key dates, funding description, research plan, award information, eligibility...aspects are understood. While reading the funding description, pay attention to the keywords used and think...project are clearly stated and align with the description of the RFA and funding institution. Emphasizing...

...spreadsheet Data Field Description Plasmid Name Use a descriptive plasmid name and match the...does this plasmid do?” in <200 characters. A descriptive purpose focuses on the practical application ...

...loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a colorimetric readout that can... 45 minutes. The team deposited the reverse transcriptase, protease, and Bst polymerase constructs from...toolkit include ones for expressing MMLV reverse transcriptase and Taq polymerase that can be used in a one-step...

...'ve overlooked regulatory fragments of these transcripts. I remember when small RNA was just something...’t give tRNAs much thought. High throughput transcriptome sequencing methods including work done by the...circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq data. As...

...histone modifications, which can influence the transcription of genes by RNA polymerase II, or by the addition...methylcytosine in CpG islands makes  genes less transcriptionally active. The role of epigenetics in studying...of tumor suppressor genes can repress their transcription and inactivate them. Global hypomethylation,...

...sequences that, when bound by transcription factors, can increase a gene’s transcription. Sometimes enhancers ...vivid chromatin picture, CAPTURE 2.0 re-writes the script on gene regulation. References Han, J., Zhang...