...using the technology in the lab (e.g., controls to perform, recommended histology conditions, minimum/maximum...

...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and...Watch the protocol video below to learn how to perform gel electrophoresis. Equipment Casting tray Well...

... AICSDP-117 mEGFP RPB1 RNA polymerase II 168798 TERF2-mEGFP AICSDP-77 mEGFP Telomeric repeat-binding factor...

...with Transient Plant Leaf Transformations RNA Interference in Plant Biology: New Tools for an Old Favorite...

...resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate...

... manipulate genetic information, viruses are a powerful tool for the delivery of this genetic information...

... Pooled Libraries Pooled libraries represent a powerful tool for forward genetic screening, or identifying...in the selection NEGATIVE screen: Apply library Perform Next Generation Sequencing (NGS) on a control sample...library Apply selection; most cells will survive Perform NGS on the surviving cells Compare NGS results ...

... is anticipated to be useful for laboratories performing phosphoproteomic experiments in need of reference...

...are prone to misfolding and aggregation and can interfere with protein-protein interactions and nerve cell...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

... (from Chloromonas oogama ), and SdChR (from Scherffelia dubia ). Synthetic variants have been created...Chloromonas subdivisa CheRiff Channelrhodopsin from Scherffelia dubia Channelrhodopsins: cation channels- chimeras... opsin is reached. This approach is especially powerful when combined with site specific recombinase technology...

...transmembrane glycoprotein of the immunoglobulin superfamily, binds to the SARS-CoV-2 S protein and is involved...

...maize, Arabidopsis, and rice. Proof-of-concept performed in zebrafish. ZiFiT Targeter Originally developed...

...Models Induced pluripotent stem cells (iPSCs) are a powerful system for generating Rett syndrome-specific differentiated...

...function by RNA interference (RNAi) RNA interference (RNAi) is a powerful tool for performing targeted knockdowns...the students performing these experiments have a good baseline for comparison. Performing WISH using these...are too few resources to teach students how to perform these experiments in the beginning. In most classrooms...observation and dissections. Students don’t usually perform more modern techniques used in genetics or molecular...addition, the Sánchez lab has made it easier to perform advanced developmental biology experiments in planarians...genes. Double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) to knockdown expression of specific gene...both WISH and RNAi experiments, as well as how to perform worm amputations. They also created an online companion...

...Implementation Plasmids References The development of powerful molecular reagents for biology have propelled ...

... vivo testing of these viral preparations was performed by the Karpova lab. Results for our quality control...

...Addgene's blog on Systemic Capsids . To review performance data of each of these serotypes, please refer...

...than one another, a two-tailed test should be performed. This test will assess if the mean of an experimental...tests, referred to as post-hoc analysis, must be performed. These will be discussed later. One and two-way...within your ANOVA/ANCOVA analysis. If you have performed a MANOVA/MANCOVA and want this information, multiple...multiple ANOVAs/ANCOVASs must be performed first, and then post hoc tests can be run on these tests. ...can reduce the family-wise error rate by only performing the comparisons you really care about. Family-wise... positive occurring when many comparisons are performed. For example, if your alpha is set at .05, then...test will be incorrect. If you have 5 samples and perform every pairwise comparison (10 in total), your likelihood...

...optimization as each antibody performs differently and some antibodies impact the performance of other antibodies...beautiful results. Performing an immunofluorescence experiment The process to perform IF can be broadly...important considerations to make when planning and performing an IF experiment. What is immunofluorescence... different species and colors makes it easy to perform multiplexed IF. By using multiple primary antibodies...in IF experiments. Precisely which controls you perform will depend on your experimental context.  Choosing... to use, then the primary antibody will likely perform as expected in both.  How do you find validated...may be, it is always important to verify their performance in your hands and in your specific experiment...