...broad): somatic and visceral sensory ganglia, type II spiral ganglion neurons Mice, rats No (mechanism...administration, distributing widely throughout the organism to target large or diffuse biological structures...specific application and animal model.  Other model organisms Tropism and efficacy can vary depending on species...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific Tropism?  Reference... and shows decreased transduction in off-target organs (Goertsen, Flytzanis, et al., 2022). AAV-PHP.N ...has been shown to transduce 80% of dorsal root ganglia (DRG) neurons, as well as neurons located in the...

... a variety of functions that allow their host organisms to thrive in competitive environments. The genes...the competition, and Penicillium isn’t the only organism that knows it. Some bacteria possess plasmid-borne...encode genes that allow the host to infect other organisms. If you’ve ever wondered why E. coli is safe to...means to deliver DNA and other molecules to host organisms. For instance, vectors derived from the Agrobacterium...like by living where no one else wants to. Some organisms manage this with plasmids that allow them to eat... eat unusual substrates that other organisms can’t use. Other plasmids offer resistance to toxic substances... which protect the host from attacks by other organisms. To conserve energy, these specialized degradative...

...from organization to organization, so you’ll need to be familiar with your specific organization/department...-2 labs. When working with lentivirus, many organizations require or recommend disposable gowns over your...cabinet, even if it is not required by their organization. It’s always okay to add another level of protection..., 10% bleach, or 1% iodine are useful. Some organizations or cities may require a higher percentage of...cut. Following all sharps procedures for your organization will significantly reduce the risk of an accident...also need to familiarize yourself with your organization’s reporting mechanisms before you start an experiment...

...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...LAD Light-activated dimerization, using proteins GIGANTEA (GI) and the light oxygen voltage (LOV) domain...engineered zinc finger proteins, using proteins GIGANTEA (GI) and the light oxygen voltage (LOV) domain...

...across organisms. When using the MK system, many of the parts can be used in a variety of organisms, saving... these applications, no matter what your model organism is. MoClo background MoClo is a clever way to...MoClo kit recommendations for different modal organisms and applications.    Plant applications We...kits: Plant Parts Kit II and Plant Parts Kit III (Gantner et al., 2018; Chamness et al., 2023). The Plant...libraries and tools available for certain model organisms and backgrounds, MoClo can be invaluable for more... complex editing and screening experiments in organisms that don’t yet have these tools built out. While...many MoClo kits are designed with specific model organisms in mind, others are designed for flexibility. ...

...professional works for or with an organization to evaluate the organization’s grant readiness, identify grant...stories about a time when their passion for an organization or a program and a funding opportunity coincided...submitting it, to evaluating the capacity of an organization to receive and manage funds.  Grant professionals...targeted to the grants profession? The number of organizations, both public and private, that qualify for grants...oriented nonprofits. Like Deb, I worked for an organization in a position that required some involvement...employer, I took on my first consulting job, and I began to realize I too had a niche. As a result, I started...your ideal client or job will save you and the organization(s) you serve time and resources. 3. Get a mentor...

...Why have zebrafish become such a popular model organism? Zebrafish (Figure 1) were initially recognized...reasons that zebrafish have been embraced as a model organism, I’ll outline a few of the available genetic resources...reason for the success of zebrafish as a model organism is its amenability to genetic manipulation. Tools...Europe, and China. In addition, the Zebrafish Model Organism Database ZFIN actively curates information on ...Generating zebrafish transgenics Creating transgenic organisms by introducing foreign DNA into the genome is ...fertilized zebrafish zygote transforms into an organism with a clearly established body plan including...pinhead—contribute to the “sculpting” of tissues and organs during embryo development. Single cell sequencing-based...

...common means to achieve ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain (LDB...interest (Atasoy et al. 2008; Cardin et al. 2009). Ligand regulated recombinase - keeping Cre out until needed...specific time point? To allow temporal control, ligand regulated Cre and FLPe recombinase have been developed...retained in the cytoplasm until estrogen receptor ligand Tamoxifen is added. Tamoxifen then binds to the...versions can be used in these experiments. This elegant combination of Cre, FLP, and inducible systems ... 6. Logie, Colin, and A Francis Stewart. 1995. “Ligand-Regulated Site-Specific Recombination.” Proc. Natl...Site-Specific Recombination in Mammalian Cells Using a Ligand-Dependent Chimeric Cre Recombinase.” Proc Natl ...

... with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility. The minisymposium... additional resources and information about organizations promoting reproducibility in this booklet (which...within a published manuscript. Luckily, many organizations have been working to provide tools that make...expand to other protein technologies later. Organizations such as BenchSci and FPbase are working hard... they’ve used for data analysis while other organizations like Dryad make it easier for researchers to...are blogs - both hosted by individuals and by organizations like Addgene (a good example is our series of...editing technology NgAgo). In addition, there are organizations like ReFigure which aims to consolidate published...

...has taken place in other organisms. In many bacteria, unlike other organisms, CRISPR-induced double stranded...diseases have connections to either specific organisms or organism/gene interactions with the host. This scenario...can consider the microbiome as a collection of organisms, their genes, and metabolic processes, occupying...to diseases where the presence of just a single organism, such as Clostridium difficile, could be targeted... This approach successfully targeted specific organisms when using bacteriophages or mobile plasmids in...Pseudomonas species Expanding this tool to other organisms, the Ji lab brought genome and base editing to...genome engineering remains challenging for most organisms in the genus. Work from the Sun lab demonstrates...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in research... there were other academics and like-minded organizations with similar ideas who were also thinking about... an eLife ambassador, had previous experience organizing open access events and grassroots efforts. After...in developing reproducibility workshops and organizational skills. Meanwhile, Addgene was also thinking... in the year and in the fall of 2018, I helped organize a workshop for community scientists at the Global... would want to know before, during, and after organizing such a workshop. Finally, the group put a lot...IS-MPMI. If you’re interested in having a workshop organized at your upcoming conference or you’d like to join...

...Neuroendocrine Tumors Using Adult Stem Cell-Derived Organoids Matthew Meyerson Dana-Farber Cancer Institute ...

...Girardia sp., Dugesia dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different...developmental biologists have to extract RNA from their organism of interest, use the RNA to create a complementary... in  the labeling specific cell types or entire organs in planarians. For example, the gene Piwi labels...discovered and characterized in an unrelated worm (C. elegans) [2]. Delivering dsRNA matching a specific mRNA...strain that is designated as DE3. For Phagocata morgani and Phagocata gracillis, the process is a little...E. coli. Thus, to get the dsRNA into Phagocata morgani and Phagocata gracillis, one has to purify the ...interference by double-stranded RNA in Caenorhabditis elegans. Nature, 391 (1998), pp. 806-811. PubMed PMID: ...

...models of C. elegans molecular biology are based on the standard worm genome. While C. elegans are often ...of active spaces near you.   Learning about C. elegans with the OpenWorm Project One such space is the...project. The main goal of OpenWorm is to simulate C. elegans as a computer model. When I first heard about it...often thought of as simple organisms , they have many levels of complexity, especially when trying to explore...and understand this complexity using existing C. elegans mutants. For instance, we use a mutant known as...collection of samples and sequencing is being organized and funded by someone else (KittyBiome has been...

...educating the public and organizing events. I contacted local professional organizations and nonprofits. I volunteered...Central Indiana Science Outreach, or CINSO. We organize fun science events for adults and professional... test and discover what career suits you best I began viewing my activities as types of experiments. I...communication, editing, marketing, and website organization. My short experience with my blog helped immensely...establishments such as museums, professional organizations, and other nonprofits to gain support for CINSO...graduate experience, the point at which my career path began to take shape, was when I started volunteering. ...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

... priority and have organized many events over the years. To keep the organizational side of things from...Use it! 8. Organize If you’re doing an event that requires people to be in groups, organize your troops...event! Addgene company culture At Addgene we organically developed the philosophy of  “work hard, play... in getting the fun started. I’m aware that  “organizing the fun” sounds a little neurotic, but hey- sometimes... her operational responsibilities, she enjoys organizing team building events at Addgene and cultivating...