We narrowed to 89 results for: grna cloning vector
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TypeCollection...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...popular empty backbone plasmids into which you can clone your regulatory element or gene of interest into... of 5' promoter/enhancer regions using Gateway cloning. Jorge Ferrer 16539 pBV-Luc Firefly Insertion of...Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream...Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc NanoLuc...Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB...Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly...
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Tetracycline Inducible Expression
TypeCollection...plasmid. A single vector approach may not have as much flexibility and control as a dual vector design, but ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or search...tet-inducible vectors. Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control....1 Lentiviral Tet-On vector for inducible expression; insert with Gateway cloning and puromycin selection...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...respectively. You should also choose between: Dual vector system: express the transactivator and the tet-...-controlled gene of interest from two separate vectors. Stable cell lines or transgenic animals can be... -
Plan Your Experiment
TypeGuide...the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit....more about how to design your gRNA . Synthesize and Clone Desired gRNAs Once your target sequences are...it’s time to design your gRNA oligos and clone them into your desired vector. In many cases, you can synthesize...containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact cloning strategy...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral...Introduction Select Genetic Manipulation Design and Clone gRNA Select Expression and Delivery Validate Genetic... -
CRISPR Guide
TypeGuide...come in 1-vector systems, in which Cas9 is included in the gRNA-containing plasmid, or 2-vector systems,... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ...sequence is cloned into a gRNA expression plasmid but does NOT include the PAM sequence or the gRNA scaffold... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...amplify a specific sequence of DNA pegRNA P rime E dit gRNA; a specialized gRNA containing an additional primer...Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20 nucleotide... -
Lentiviral Vector Guide
TypeGuide...Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations Vector Production...lentiviral plasmids. Lentiviral Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into...Viral Vectors 101 topics More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols... require gRNAs for each target. Lentiviral vectors are a common way of delivering these gRNAs, due to ...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors have ...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...gamma-retroviruses, see Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically... -
Adenovirus Guide
TypeGuide...Guides Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production Integration... rAdV vectors and helper virus. Created with BioRender.com. Adenoviral Vector Production Cloning Cloning...capacity of rAdV vectors (~6.5 kb for first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb)...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...adenoviral (rAdV) vectors in order to deliver genetic cargo into cells. Most rAdV vectors are based on adenovirus...Generations First-generation rAdV vectors The first generation of rAdV vectors was developed by removing the... -
Addgene's eBook Collection
TypeGuide...viral vectors contains an introduction to each vector and its applications, tips for using vectors in your...Overlay Select Viral Vectors 101 July 2024 | Second Edition About Viral Vectors 101 July 2024 | ...nomenclature, and uses to common promoters and cloning techniques! Close About Overlay Select CRISPR 101...planning your CRISPR experiment and designing your gRNA and how to use pooled libraries in genome-wide screens... -
Adeno-associated virus (AAV) Guide
TypeGuide...Guides AAV Vector Guide Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production...for rAAV vector production. Created with BioRender.com. AAV Vector Production Cloning Cloning your transgene...Read our adeno-associated viral vector guide to learn about AAV vector components, production and common...used as viral vectors for gene therapy applications. This guide contains many viral vector-specific terms...adenovirus, read our Adenoviral Vector Guide . Recombinant AAV Vectors Wild-type AAVs have been modified...transgene, gRNA, or shRNA of interest into the transfer plasmid can be done with most standard cloning methods...compatible with certain cloning methods (such as a Gateway destination vector), so be sure to confirm ... -
Gamma-Retroviral Vector Guide
TypeGuide...Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral Vectors Vector Production...BioRender.com. Gamma-Retroviral Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...of Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...Gamma-retroviral vectors do not have defined “generations” of plasmids, as lentiviral vectors do. For more...lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral Vectors The genome of gamma-retroviruses...