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Showing: 81 - 100 of 879 results

  1. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ... the tag. Created with BioRender.com. Plasmids, plasmids everywhere To find out more about gene edited...expensive for many labs. We’ve created and tested plasmids that use CRISPR/Cas9 to endogenously tag a wide...wide variety of genes with GFP. These plasmids are available through Addgene, and the stem cell lines we...found in the Cell Catalog on our data portal. The plasmids should be functional in many different human cell...constructs below. Read on to find out how these plasmids work and to discover more resources for your genomic...BioRender.com. The first step in creating the plasmids was to identify the gene of interest and decide...this manner, we have created an initial set of 10 plasmids representing 10 human genes and will add more ...

  2. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...head over to our CRISPR Plasmids and Resources page.  Plasmids for delivering CRISPRi components by a single...'ll make it easier for you to find and use the plasmids you need. Here's what you'll find in this post...inducible degron system for C. elegans New CRISPR plasmids New items from the viral service Epigenetic ... Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic researchers...proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence) tagged proteins...

  3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...possible, we propagate plasmids in the standard cloning strain DH5α. For plasmids with highly repetitive...lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain. For plasmids containing the ccdB gene...username. In order for the plasmids to appear in your Addgene account under My Plasmids, we need to know your...quickest ways to submit deposits with more than 10 plasmids is to use our deposit spreadsheet?  Our plasmid...Material Transfer Agreement (MTA) approval  Send plasmids to Addgene  Quality control  In this post, we...to send liquid DNA or bacterial streaks of your plasmids back to us. Our scientists will contact you if...Figure 1). Please read our “How to Deposit Your Plasmids with Addgene” blog post for a thorough guide to...

  4. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ... for More Plasmids 101 Articles?  Find them all here: http://blog.addgene.org/topic/plasmids-101   ...In a previous Plasmids 101 blog, we reviewed the salient features of several popular strains of E. coli...strain? Protein expression from high-copy number plasmids and powerful promoters will greatly exceed that...BLR (DE3) Tetracycline RecA-deficient; best for plasmids with repetative sequences.  F- ompT lon hsdSB... strains is not recommended as these auxillary plasmids may be isolated during the prepping process. How...expression work? As mentioned above, many expression plasmids utilize inducible promoters, which are 'inactive...replication, making it incompatible with other p15A plasmids. pLys comes in two flavors—pLysS and pLysE—the...

  5. Plasmids 101: Common Lab E. coli Strains

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    Blog Post
    ...Vectors Looking for More Plasmids 101 Articles?  Find them all at our Plasmids 101 Topic Page!     ...synthesis Ptrc-ccdA   Propagation of ccdB-containing plasmids Hte   High transformation efficiency deoR ...for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency supE44...Survival 2 T1R   TOP10 derivative. For propagating plasmids expressing the ccdB gene (important in Gateway...Streptomycin  HB101 derivative. For propagating plasmids expressing the ccdB gene (important in Gateway...DH5alpha   General cloning and storage of common plasmids, blue/white screening. F- endA1 glnV44 thi-1 recA1...Cloning large inserts and transformation of large plasmids. F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74...

  8. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post
    ...that took up the vector and distinguish between plasmids that contain the gRNA (white) from those that ... it turns into an insoluble blue pigment. Find plasmids for blue-white screening at Addgene! Some bacterial...way to distinguish between colonies containing plasmids with inserts from those that do not is by using... video on using restriction digests to analyze plasmids, which can help you visualize this process, or...Addgene blog Get all the plasmid basics in our Plasmids 101 series Find more molecular biology protocols...

  9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

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    Blog Post
    ...plasmid DNA. Among those that do, some now have plasmids that contain your gene of interest, but others...kanamycin-containing agar. Only cells carrying plasmids that contain a kanamycin resistance cassette will... for cloning are auxotrophic for specific amino acids. When plated on media lacking the essential amino... created a yeast prototrophy kit containing 23 plasmids to complement auxotrophy. Negative selection: ... property by including ccdB on its destination plasmids. In the destination plasmid, the ccdB gene is ...Additional resources on the Addgene blog Read our Plasmids 101 blog posts Learn more about Lambda Red recombineering...counter-selection Resources on Addgene.org Find plasmids at Addgene Read our molecular biology reference...

  10. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ... Gulp! Though bacteria don't exactly "eat" plasmids as depicted in this comic, when a bacterium uptakes...’s genome. To do this scientists commonly use phagemids, a DNA cloning vector that contains both bacteriophage...bacteriophage and plasmid properties. The phagemids are packaged into replication-incompetent phage particles...find all kinds of different lentiviral and AAV plasmids as well as ready-to-use viral preparations at ...requires the use of a fine needle to deliver nucleic acids to individual cells. Electroporation on the other...resources on the Addgene blog Read all of our Plasmids 101 blog posts Check out all of our blog posts...

  11. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...underrepresented plasmids (plasmids that were lost or “dropped out”) and overrepresented plasmids (plasmids that ...library contains many plasmids, care must be taken to ensure that all of these plasmids are amplified and ...diversity. If the various plasmids in the library are differently sized, smaller plasmids may be amplified preferentially...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds...hundreds, thousands, or even a million plasmids. These libraries are some of Addgene’s most exciting and versatile...growth of other cells. For libraries where the plasmids are all very similar sizes, like a gRNA library...2. You can use a small PCR product because the plasmids in the library are identical except for the insert...

  12. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    ...Addgene “Find Plasmids” badge in the image below. If an abstract on PubMed has the “Find Plasmids” badge, you...you’ll find new plasmids. Simply click on the badge to be taken directly to the plasmids on Addgene’s site...you should see the “Find Plasmids” box on papers that have deposited plasmids. Now that you know how ...Order Plasmids from Addgene Check Out Our FAQ Pages Browse Plasmids ...thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed searching...you can use PubMed to see which articles contain plasmids deposited at Addgene from the abstract page. Check...article to ensure you understand the features of the plasmids, how they were created, and how to use them in...

  13. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...Addgene Blog Plasmids 101: Common Lab E. coli Strains Plasmids 101: TOPO Cloning Plasmids 101: Golden ...SurvivalTM, one can efficiently propagate and prep plasmids containing the ccdB gene, which can then be used...difficult to transform, depending upon the specific plasmids being used. We would also like to note that while...should never be used when selecting for recombinant plasmids. Check out our blog post on Common Lab E. coli...

  14. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...researcher at a nonprofit institution, you can order plasmids covering a wide range of fields from Addgene. ...that Addgene distributes curated collections of plasmids as kits with greatly reduced costs per plasmid...create kits to allow you to easily use a set of plasmids together on a tight budget. To help popularize... production (a variety of pExpression vectors) plasmids. The type IIS restriction sites used in these ...products. Negative selection markers built into all plasmids in the kit help decrease false positives observed...to a variety of experimental opportunities. The plasmids in this kit have been used to purify a variety... BIOFAB and the Endy Lab created this kit. The plasmids within it contain a variety of different promoters...

  15. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

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    Blog Post
    ...hot plasmids in our newsletter or on our hot plasmids webpage. Listen to the entire Hot Plasmids podcast...In the second episode of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins...podcast! Hot plasmids covered in this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly...Receive Our Newsletter Check Out All of Our Hot Plasmids Articles Browse the Repository  ...

  16. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...with the Plasmid Name, Addgene Plasmid ID #, and the PubMed ID # (PMID) for the article that has used ...it has been validated in.  If a plasmid's Addgene ID # has been referenced in other publications, you'...your deposited plasmids Increased visibility of your plasmids in the "Associated Plasmids" lists Benefits...Find other related plasmids in the "Associated Plasmids" lists Properly citing plasmids you've requested... from Addgene Plasmids 101: How to Verify Your Plasmid Plasmids 101: Control Plasmids 6 Tips for Analyzing...'ll find the plasmids listed below the citation. An example of these "Associated Plasmids" is shown in...assistance finding plasmids on Addgene's website or would like to contribute plasmids you have made to ...

  17. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...collection of plasmids is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent...Michelle Baird, who has created plasmids as a technician in the Davidson lab for the last four years. “...mEmerald plasmids offer a comprehensive list of readily available targets since the Davidson lab always...work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael Davidson, Michelle Baird...and their use for imaging cell biology, Michael Davidson’s lab at Florida State University has been the ...Molecular Expressions collection including some 3,300 plasmids along with image galleries and educational resources...Fluorescent Protein Collection webpage. These plasmids are divided into the following categories: Over 300 ...

  19. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

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    Blog Post
    ...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control...controls prescribed by the Australia Group, for these plasmids for the majority of the countries we ship to, ...lasting basis.   Table 1: Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G ...control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster, as ...required. If you have any questions on ordering plasmids, please feel free to email [email protected]. We...

  20. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...'ll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a ... Figure 1: The base editor is split into two plasmids and co-transfected into cells for reconstitution...overcome this overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion...channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed...fragments. This system has been used to construct plasmids for use in model organisms such as E. coli and...for cyanobacteria. Figure 3: Assembly of plasmids using the Cyanogate Kit and subsequent transformation...
Showing: 81 - 100 of 879 results