We narrowed to 677 results for: ids
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TypeBlog PostPublishedNov. 20, 2018, 8:17 p.m.... contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene...Addgene IDs in published work allows readers to quickly track down the specific plasmids used. RRIDs and ...use in our labs, including plasmids. All plasmids at Addgene have unique ID numbers associated with them...them, which serve as unique PIDs within the Addgene catalog. These IDs allow you to unmistakably identify...the “Articles Citing this Plasmid” section. Having IDs linked to these metadata and publications help to...about each plasmid item. Given a specific Addgene ID # listed in a publication, you can quickly pull up...look at lenti-Cas9-VQR-Blast, which has the Addgene ID #87155. The resolvable compact identifier format ...
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Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....plasmid-based experiments. What are control plasmids? Generally, control plasmids help to ensure that the observed...delineate its requisite control plasmids, and discuss why these control plasmids are critical for the correct...proper control plasmids would address and answer these questions. Types of control plasmids Part of planning... and not some other unintended factor. Control plasmids are used to minimize any effects of the non-independent...variables. To illustrate the importance of control plasmids, we will take a step beyond discussion in the ...non-targeting control Negative control conditions and plasmids should produce a null effect (i.e., no phenomenon...target cells. Positive controls Positive control plasmids should produce the expected phenomenon. One example... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....DNA designed in the lab. Specially constructed plasmids can be used to replace genes in yeast, mice, or...gene. Here, we'll describe the techniques and the plasmids used to inactivate specific genes in mammalian...mind when choosing a knockout strategy! Knockout plasmids Homologous recombination is a mechanism to accurately...Cre-mediated recombination. Knockout/Knock-in plasmids Gene targeting methods also make it possible to.... Conditional knockouts The methods and plasmids described in the preceding sections are simple... of Cre recombination. The future of knockout plasmids Although these methods have been used to create...Find Mammalian Expression Vectors Find Cre-Lox Plasmids Learn More about CRISPR ... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....colicin-producing plasmids have been referred to as “Col plasmids” or “Col factors.” Virulence plasmids: microbial...Mother Nature’s toolbox. Natural plasmids, also called environmental plasmids, are all around us! A plasmid...., 2001) Beneficial traits of natural plasmids Natural plasmids often convey a variety of functions that...functions of natural plasmids. Created with BioRender.com. Resistance (R) plasmids: microbial defense...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria ...cells. Cryptic plasmids: microbial mysteries Cryptid hunters watch out — cryptic plasmids are real! This... term refers to plasmids that don’t have known functions. While some of these plasmids likely have undiscovered... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....host range in plasmids? Not all plasmids were created equally. Only a handful of plasmids have been identified...Addgene blog Plasmids 101: Origin of Replication Plasmids 101: What is a Plasmid? Plasmids 101: Transformation...within different host organisms. pBBR1-based plasmids and plasmids in incompatibility groups N, P, Q, and W...Understanding the characteristics of natural plasmids and how they function in nature can inform us on...of the features that confer broad host range in plasmids in nature, and provide tips on how we can harness...transfer to, replicate in, and persist in. Some plasmids have a narrow host range, while others have a ...Recombinant vectors that are made from broad host range plasmids are more stable, can be easily cloned in different... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m....“plasmid” derived as a hybrid of "cytoplasm" and "id" (Latin for 'it'), as “a generic term for any extrachromsomal...Learn more about the history of plasmids and cloning: CSHL Meeting: Plasmids: History & Biology The Joshua...factors played within an organism. So how did plasmids get their name? In 1952, Joshua Lederberg set...in the early 1950s, it took until the 1970s for plasmids to gain prominence in the scientific community...eventually mammalian) species were cloned into plasmids and new cloning vectors such as pBR322, pACYC,...be used in these cloning experiments. Although plasmids started as a somewhat niche area of research, ... research! Since their discovery in the 1950s, plasmids have greatly impacted many areas of biological... -
Plasmids 101: Biotinylation
TypeBlog PostPublishedNov. 15, 2018, 1:50 p.m....catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites. Biotin...biotinylating proteins with BirA. You can find plasmids that contain both AviTag and BirA at Addgene! ...becomes reactive and forms a link with nearby nucleic acids (McInnes et al., 1990). Photobiotinylation is sometimes...dsDNA, ssDNA or RNA–protein interactions.” Nucleic Acids Research, 23.18 (1995): 3792–3793. PubMed PMID: ... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m.... (blue) light switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use ...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ... or want to swap out the constant domains, the plasmids for the heavy chain and light chain are also available...minimal background. Find these C. elegans mScarlet plasmids here! Cao W.X., Merritt D.M., Pe K., Cesar ...Neo et al., 2024 under CC-BY license. Find plasmids for CRISPR/Cas9 in M. abscessus here! Neo D, ...with a time resolution of minutes. Find PILATeS plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., ... -
Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m....of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. ...be used for applications beyond cryo-EM. Find plasmids for MagIC-Cryo-EM here! Arimura, Y., Konishi...disease phenotype in an animal model. Find PE-eVLP plasmids here! The lab’s other recent deposit builds on...over the wild-type enzyme. Find optimized PASSIGE plasmids here! Figure 3: Schematic of the PASSIGE...without compromising their efficacy. Find CHARM plasmids here! Neumann, E. N., Bertozzi, T. M., Wu, E... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. ...including in human retinal organoids and live mice. Importantly, REVeRT avoids the introduction of extraneous... over the original CROPseq. Find CROPseq-multi plasmids here Walton, R.T., Qin, Y., Blainey, P.C. (2024...potential improvements to the editor. Find PE7 plasmids here! Figure 2: PE7 improves prime...conjugated with its HaloTag domain. Find Voltron2 plasmids here! Abdelfattah, A.S., et al. (2023) Neuron...therapeutic applications. Find microglia-targeting AAV plasmids here! Serrano, C., et al. (2023). Simple and ...potential for therapeutic success. Find REVeRT plasmids here! Riedmayr, L.M., et al. (2023). mRNA trans-splicing... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint Gene Tagging Plasmids 101 eBook Resources ...Resources on the Addgene blog Plasmids 101: Protein Tags Plasmids for Endogenous Gene Tagging in Human Cells ... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....NEB’s DAD Interview with Nicola Patron on MoClo Plasmids 101: Golden Gate Cloning ... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...Figure 3 Colognori et al 2022. Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise...inducible expression. Find heat-inducible lentiviral plasmids here! Wu Y, et. al. Control of the activity ... Campbell et al, Nature Methods Find YFP plasmids here! Campbell, B.C., et al. Chemically stable... -
Plasmids 101: Dimers and Multimers
TypeBlog PostPublishedJan. 10, 2023, 2:15 p.m....Addgene blog: Plasmids 101: What is a plasmid? Plasmids 101: Origin of ReplicationPlasmids 101: PCR... the sequencing results for their plasmids, they found the plasmids were much longer than expected, and...When performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our...full plasmid sequencing services to verify the plasmids, including long-read sequencing techniques, such...was repeated two or more times in tandem. Such plasmids are known as multimers, and they are the result...been observed to occur in many different kinds of plasmids, taking many forms (Levene, 2009; Higgins & Vologodskii... has been observed to occur more frequently in plasmids with large inserts (>7 kb)(Berg et al., 1989),... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m....Tet-Off system. For more information on Addgene Tet plasmids, see our Tetracycline resource page. Cumate Switch...20697123. PubMed Central PMCID: PMC2942869. Find plasmids from this publication at Addgene. del Valle Rodriguez...20434990. PubMed Central PMCID: PMC2883883. Find plasmids from this publication at Addgene. Wei, Xing, ...22406855. PubMed Central PMCID: PMC3846601. Find plasmids from this publication at Addgene. Riabinina, ...25581800. PubMed Central PMCID: PMC4344399. Find plasmids from this publication at Addgene. Riabinina O... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m. -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m....modifications Learn more molecular biology in our Plasmids 101 eBook! Researchers use various techniques ...RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems are ideal for fast...assemble proteins with labelled or modified amino acids that are useful in different downstream applications...being able to help the scientific community share plasmids. Additional Resources Thermofisher Protein ...Addgene.org Bacterial expression systems Search for plasmids by expression system ... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....Resources on the Addgene Blog: Plasmids 101: The Promoter Region Plasmids 101: Cre-lox Light-Switchable...Systems for E. coli Browse other blog posts from our Plasmids 101 series Resources on Addgene.org: Inducible...Inducible Systems at Addgene Cre-lox Guide and Plasmids ... -
15 Hot Plasmids from 2017
TypeBlog PostPublishedJan. 9, 2018, 3:02 p.m....editing for precise genome modification. Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles... you to find and use the plasmids you need. You can find all the hot plasmids from 2017 below. With over...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...articles provide a synopsis of a plasmid or group of plasmids' functions and applications. We hope that these...over 50,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but...this cell type. To this end, they constructed AAV plasmids bearing one of the distal-less homeobox 5 and ...