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We narrowed to 877 results for: ids

Showing: 1 - 20 of 877 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...protocol for volume of bacteria to grow (low copy plasmids require larger cultures). Centrifuge the culture... DNA to precipitate, while leaving the smaller plasmids free in solution. Pellet the proteins and genomic...

  2. Lentivirus ddPCR Titration

    Type
    Protocol
    ...green) and negative (black) droplets Sample ID Plasmid ID Dilution Copies/20 µL RRE (FAM) Copies/20 µL...

  3. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ... contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene...Addgene IDs in published work allows readers to quickly track down the specific plasmids used. RRIDs and ...use in our labs, including plasmids. All plasmids at Addgene have unique ID numbers associated with them...them, which serve as unique PIDs within the Addgene catalog. These IDs allow you to unmistakably identify...the “Articles Citing this Plasmid” section. Having IDs linked to these metadata and publications help to...about each plasmid item. Given a specific Addgene ID # listed in a publication, you can quickly pull up...look at lenti-Cas9-VQR-Blast, which has the Addgene ID #87155. The resolvable compact identifier format ...

  4. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...plasmid-based experiments. What are control plasmids? Generally, control plasmids help to ensure that the observed...delineate its requisite control plasmids, and discuss why these control plasmids are critical for the correct...proper control plasmids would address and answer these questions. Types of control plasmids Part of planning... and not some other unintended factor. Control plasmids are used to minimize any effects of the non-independent...variables.  To illustrate the importance of control plasmids, we will take a step beyond discussion in the ...non-targeting control Negative control conditions and plasmids should produce a null effect (i.e., no phenomenon...target cells. Positive controls Positive control plasmids should produce the expected phenomenon. One example...

  5. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...DNA designed in the lab. Specially constructed plasmids can be used to replace genes in yeast, mice, or...gene. Here, we'll describe the techniques and the plasmids used to inactivate specific genes in mammalian...mind when choosing a knockout strategy! Knockout plasmids Homologous recombination is a mechanism to accurately...Cre-mediated recombination.   Knockout/Knock-in plasmids Gene targeting methods also make it possible to....   Conditional knockouts The methods and plasmids described in the preceding sections are simple... of Cre recombination.  The future of knockout plasmids Although these methods have been used to create...Find Mammalian Expression Vectors Find Cre-Lox Plasmids Learn More about CRISPR  ...

  6. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...colicin-producing plasmids have been referred to as “Col plasmids” or “Col factors.” Virulence plasmids: microbial...Mother Nature’s toolbox. Natural plasmids, also called environmental plasmids, are all around us! A plasmid...., 2001) Beneficial traits of natural plasmids Natural plasmids often convey a variety of functions that...functions of natural plasmids. Created with BioRender.com. Resistance (R) plasmids: microbial defense...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria ...cells. Cryptic plasmids: microbial mysteries Cryptid hunters watch out — cryptic plasmids are real! This... term refers to plasmids that don’t have known functions. While some of these plasmids likely have undiscovered...

  7. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...host range in plasmids? Not all plasmids were created equally. Only a handful of plasmids have been identified...Addgene blog Plasmids 101: Origin of Replication Plasmids 101: What is a Plasmid?  Plasmids 101: Transformation...within different host organisms. pBBR1-based plasmids and plasmids in incompatibility groups N, P, Q, and W...Understanding the characteristics of natural plasmids and how they function in nature can inform us on...of the features that confer broad host range in plasmids in nature, and provide tips on how we can harness...transfer to, replicate in, and persist in. Some plasmids have a narrow host range, while others have a ...Recombinant vectors that are made from broad host range plasmids are more stable, can be easily cloned in different...

  8. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...“plasmid” derived as a hybrid of "cytoplasm" and "id" (Latin for 'it'), as “a generic term for any extrachromsomal...Learn more about the history of plasmids and cloning: CSHL Meeting: Plasmids: History & Biology The Joshua...factors played within an organism.  So how did plasmids get their name? In 1952, Joshua Lederberg set...in the early 1950s, it took until the 1970s for plasmids to gain prominence in the scientific community...eventually mammalian) species were cloned into plasmids and new cloning vectors such as pBR322, pACYC,...be used in these cloning experiments. Although plasmids started as a somewhat niche area of research, ... research! Since their discovery in the 1950s, plasmids have greatly impacted many areas of biological...

  9. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites. Biotin...biotinylating proteins with BirA. You can find plasmids that contain both AviTag and BirA at Addgene! ...becomes reactive and forms a link with nearby nucleic acids (McInnes et al., 1990). Photobiotinylation is sometimes...dsDNA, ssDNA or RNA–protein interactions.” Nucleic Acids Research, 23.18 (1995): 3792–3793. PubMed PMID: ...

  10. Let There Be LITE Plasmids

    Type
    Blog Post
    ... (blue) light switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use ...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest...

  11. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...compatibility with one or more other plasmids. Generally speaking, plasmids with the same ori are incompatible...a rule, plasmids from the same group should not be co-transformed, so if you need two plasmids for an ...into phage particles. Plasmids with phage-derived ori's are referred to as phagemids.   Other factors ... used to propagate plasmids, but endA- E. coli are best for high yields of plasmids. Growth conditions...must to survive within cells. The replicons of plasmids are generally different from the those used to...amounts? It is always good to keep in mind that plasmids with low to medium copy numbers can still express...compatibility grouping is an arbitrary designation, and plasmids from the same incompatibility group should not...

  12. Hot Plasmids - October 2020

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    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...'ll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a ...head over to our CRISPR Plasmids and Resources page.  CasPhi is 70 kDa Cas protein identified exclusively...offers advantages for cellular delivery. Get the plasmids at Addgene Sc++ is a codon-optimized, engineered...nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene The Human GlycoGene CRISPR Library...can be used in Drosophila melanogaster. Get the plasmids at Addgene The Cas Hybrid for Multiplexed Editing...

  13. Hot Plasmids - August 2020

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...They generated plasmids tagged with EB3 to mark the growing tips of microtubuli and plasmids tagged with ...and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for piggyBac gRNA delivery...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head... Find the plasmids at Addgene The MoClo CRISPR/Cas Toolkit for plants includes 95 plasmids consisting...'ll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a ...and might be useful for your studies.  Find the plasmids to set up your screening systems at Addgene! ...

  14. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...cloning plasmids, expression plasmids, gene knock-down plasmids, reporter plasmids, viral plasmids, and ...plasmid lovers - serves as an introduction to plasmids. Plasmids 101 will provide you with an overview of ...cloning site. The ease of modifying plasmids and the ability of plasmids to self-replicate within a cell ...bacteria from which plasmids are isolated grow quickly and make more of the plasmids as they grow, scientists....     How do scientists use plasmids? Generally, scientists use plasmids to manipulate gene expression...and genome engineering plasmids. Some of the many things that plasmids can be used to do include: Produce...quantity inside a cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor...

  15. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...inability of different plasmids to be maintained in one bacterial cell. In this Plasmids 101 post, we’ll cover...First, why are plasmids incompatible? Plasmid incompatibility occurs when multiple plasmids within one cell...Replication? See our Plasmids 101: Origin of Replication blog).  The replication problem Plasmids with the same...with low copy plasmids, though the partitioning problem does occur in high copy plasmids (Diaz et al.,...on each plasmid and pair the plasmids together (akin to sister chromatids coming together in eukaryotic...worth noting that plasmids can be symmetrically incompatible, meaning that both plasmids are lost from the...that is easy to use with other plasmids.  How do you know if your plasmids are incompatible? Scientists ...

  16. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...all plasmids from the article! Furlanis, E., Dai, M., Leyva Garcia, B., Tran, T., … Dimidschstein, J.... synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A., Negrean, A., Chen, Y., Iyer... swap sequences effortlessly.  We built these plasmids for flexibility and speed, so you can design, ... year!  Find Addgene's own Lentiviral backbone plasmids here! Figure 2: Addgene cloned EGFP into...previous integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger, L., Lim, J., Hiraizumi...-BY-NC-ND 4.0 International license. These plasmids are part of the BRAIN Initiative Armamentarium...

  17. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... Resistance Gene Plasmids 101: Yeast Vectors Plasmids 101: Mammalian Vectors Plasmids 101: The Promoter...blog Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection Plasmids 101:...showing how DNA sequences are translated into amino acids. And I was astonished that the molecular language... How are shuttle vectors different from other plasmids? If we all use the same molecular code, can I ...prokaryotic growth. Since there are no natural mammalian plasmids, mammalian vectors are even less similar to bacterial...contain it. Antibiotic selection Most bacterial plasmids used in the lab contain an antibiotic resistance...Viral Vectors 101 series. Are broad host range plasmids shuttle vectors? While it’s technically accurate...

  18. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...selectivity. With 10 plasmids available as a kit, plus 21 optional accessory plasmids, the collection targets...precise plant breeding. Find dicot prime editing plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song... proteins for mammalian cell expression. These plasmids will be useful not only for future studies of ...proteins of interest too! Find CLIP, CLAP, and SNAP plasmids here! Smith, E.R., Turner, E.D., Abdelhamid, ...al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent versions that...potential, ASAP5 is a great new option. Find ASAP5 plasmids here! Hao, Y. A., Lee, S., Roth, R. H., Natale...

  19. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ... or want to swap out the constant domains, the plasmids for the heavy chain and light chain are also available...minimal background.   Find these C. elegans mScarlet plasmids here!   Cao W.X., Merritt D.M., Pe K., Cesar ...Neo et al., 2024 under CC-BY license.   Find plasmids for CRISPR/Cas9 in M. abscessus here! Neo D, ...with a time resolution of minutes.  Find PILATeS plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., ...

  20. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. ...be used for applications beyond cryo-EM.   Find plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi...disease phenotype in an animal model. Find PE-eVLP plasmids here! The lab’s other recent deposit builds on...over the wild-type enzyme. Find optimized PASSIGE plasmids here!     Figure 3: Schematic of the PASSIGE...without compromising their efficacy.   Find CHARM plasmids here!   Neumann, E. N., Bertozzi, T. M., Wu, E...
Showing: 1 - 20 of 877 results