We narrowed to 318 results for: idua

1. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   ... rather than the day-to-day details. Ignoring individual differencesManaging a team isn’t “one size fits...learned that my approach needed to adapt based on individual needs. For instance, I had one colleague who ...

2. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   ...everything else dies off. For a screen, you would individually treat cells with many different drugs and then... forward screens. A given panel of drugs are individually administered and then outcomes are measured....

3. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   ...really confident about working for most genes and individual targeting modules. Those two things, in my opinion...cellular consequences of repressing or inducing individual transcripts. The activator (CRISPRa) sgRNA library...

4. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

   Type

   Blog Post

   ...anywhere. The team should initially consist of 2-3 individuals that are aligned in vision and outcomes. The ...initially include a visionary, a science-minded individual, and a specialist. Notably, the team must have...

5. CRISPR Plasmids - Tagging

   Type

   Collection

   ...guide RNA transcript and a repair template for an individual tagging event. The kit contains 26 plasmids; ...

6. Reproducibility for Everyone: Lessons from an Open Science Collaboration

   Type

   Blog Post

   ...bring together talented and motivated groups and individuals with similar ideas to actually do something about...connect? Through networking of course! Thanks to individuals in the group who were well connected, this diverse...

7. Human Germline Editing Using CRISPR

   Type

   Blog Post

   ...pregnancy, the researchers were able to examine each individual blastomere to determine its genotype. However...regulatory and ethics committees comprised of individuals with varied backgrounds. Talking about this study...

8. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   ...established, it’s also a process that’s difficult for individual labs to complete. The nanobody based RANbody ...purification. Antibodies are difficult for an individual lab to produce - Hybridomas offer an alternative...

9. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   ...method it is not possible to follow turnover of individual proteins in situ.  For the visualization of specific...Söderberg, Ola, et al. "Direct observation of individual endogenous protein complexes in situ by proximity...

10. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    ...Gilbert et al., 2014; Konermann et al., 2014) individual genes for studies interrogating their functions...incredibly useful for the efficient generation of individual (and multiple) genetic perturbations in single...

11. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    ... of antibodies from 19 COVID-19 convalescent individuals (Tong et al., 2021). Another version of the spike...determine how effective sera from vaccinated individuals are at neutralizing the different SARS-CoV-2 ...

12. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    ...Fidelity, it turns out, might depend more on the individual sequence(s) than on how well that sequence fits...potential for mismatches, so you can redesign individual pieces as needed to improve fidelity. NEBridge...

13. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    ...takes about one day, even for six plates (576 individual plasmids)! These libraries are then QC’d, pooled...the raw data from each sample in our pool and individually assembles the reads into a single FASTA sequence...

14. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    ...develop new working relationships with these individuals who often had different styles from their predecessors...organization speaks in “one voice” whilst among individual members, many opinions may exist. I learnt to...

15. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    ...managing is not “one size fits all”. Manage the individuals in your team: learn what motivates them (keep...benefit of the team. By properly managing the individuals in your team you will have a much better chance...

16. Anatomy of a Plasmid Page at Addgene

    Type

    Blog Post

    ... publication page, and finally on down to the individual plasmid page. The depositing lab page contains...it would cost to buy each plasmid in the kit individually. If I order the plasmid, how will it be sent...

17. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    ...additional experiments investigating the role of individual genes or entire pathways in a particular disease...specific gene within the genome (see Fig. 2). Individual gRNAs are designed in silico using publicly available...

18. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    ...of genome engineering via CRISPR.  Though the individual topics are not surprising, the real value here...book left them wanting to learn more. There are individual paragraphs that may have warranted their own ...

19. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    ...viral cocktail are injected, by chance lots of individual cells will be transduced with multiple viral ...notice in figure 4b how difficult it is to trace individual cell’s axons, particularly the two green neurons...

20. MXS Chaining

    Type

    Blog Post

    ...label subcellular structures in live cells. Four individual expression constructs were created, each containing...cloning. The modules (Figure 2) are contained in individual plasmids and are each flanked by the same multiple...