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We narrowed to 315 results for: idua

Showing: 81 - 100 of 315 results
  1. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...in real time PCR, each reaction partition is individually assessed for a positive or negative (digital...resolution of digital PCR allows classification of individual target molecules based on their mutation status...
  2. RANbodies: Reporter Nanobody Fusions

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    Blog Post
    ...established, it’s also a process that’s difficult for individual labs to complete. The nanobody based RANbody ...purification. Antibodies are difficult for an individual lab to produce - Hybridomas offer an alternative...
  3. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...method it is not possible to follow turnover of individual proteins in situ.  For the visualization of specific...Söderberg, Ola, et al. "Direct observation of individual endogenous protein complexes in situ by proximity...
  4. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...pregnancy, the researchers were able to examine each individual blastomere to determine its genotype. However...regulatory and ethics committees comprised of individuals with varied backgrounds. Talking about this study...
  5. High Complexity Golden Gate Assembly with NEB’s DAD

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    Blog Post
    ...Fidelity, it turns out, might depend more on the individual sequence(s) than on how well that sequence fits...potential for mismatches, so you can redesign individual pieces as needed to improve fidelity. NEBridge...
  6. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...managing is not “one size fits all”. Manage the individuals in your team: learn what motivates them (keep...benefit of the team. By properly managing the individuals in your team you will have a much better chance...
  7. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ... publication page, and finally on down to the individual plasmid page. The depositing lab page contains...it would cost to buy each plasmid in the kit individually. If I order the plasmid, how will it be sent...
  8. Genome-wide Screening Using CRISPR

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    Blog Post
    ...additional experiments investigating the role of individual genes or entire pathways in a particular disease...specific gene within the genome (see Fig. 2). Individual gRNAs are designed in silico using publicly available...
  9. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...diluted in a series ranging from 1:2 to 1:32. Each individual step of the series is consistent at 1:2. When...the %CV of the standard curve, one averages the individual %CVs for each point on the curve. ‌The smaller...
  10. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...run the journal club. Some are led by a single individual who walks through the paper figure by figure....implications rather than diving deep into the individual data points. All formats should be an open forum...
  11. Getting to Know Expansion Microscopy

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    Blog Post
    ... within ~250 nm of each other. For reference, individual presynaptic terminals, which are jam packed with...their relative positions, allowing you to resolve individual points with a conventional, diffraction-limited...
  12. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...IgG subclasses. Instead, you can pre-incubate individual primary antibodies with secondary nanobodies ... with three IgG1 antibodies. Antibodies were individually incubated with a secondary nanobody conjugated...
Showing: 81 - 100 of 315 results