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We narrowed to 91 results for: lentiviral production

Showing: 81 - 91 of 91 results

  1. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...lines from a polyclonal pool of stable cells AAV Production in HEK293 Cells Produce adeno-associated virus...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...colonies Generating Stable Cell Lines with Lentivirus Genomically integrate your DNA sequence into a cell...

  2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...co-infection of helper viruses like adenovirus for productive infection. AAV vectors are engineered to provide... in Addgene's Viral Service Check out our AAV Production Protocol Check out our AAV Purification Protocol...technologies have been investigated including lentiviruses, adenovirus, and inorganic delivery tools such...

  3. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ... Eddy Page! Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer...Our CRISPR Guide Pages Find Ready-to-Use CRISPR Lentivirus Learn about Other Members of the Addgene Leadership...

  4. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ... observe, answer questions, and guide them to productive strategies while noting any gaps in their knowledge...has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies to undergraduates...

  5. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...their production and purification that yield high titers (see also AAV protocols for production, purification...tracing compounds either produce a colorimetric product or are fluorescent. While dyes usually spread unidirectionally...). AAV does not have a membrane envelope like lentivirus, but instead is contained by a capsid which is...

  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...PMC2957237. Okada, T. (2013). Efficient AAV Vector Production System: Towards Gene Therapy For Duchenne Muscular...? Check out Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources...

  7. CRISPR Guide

    Type
    Guide
    ... libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147...

  8. Lab to Office Culture Shock

    Type
    Blog Post
    ...on my technical skills with cloning and lentivirus production, I didn’t really start to recognize my transferable...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal...complex III is required for hypoxia-induced ROS production and cellular oxygen sensing. Cell Metab. 2005...

  10. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...red pH indicator) because the buildup of waste products could be suboptimal for cell growth. This conditioned... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...
Showing: 81 - 91 of 91 results