We narrowed to 151 results for: lentiviral vector plasmid

...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...virus protocols . Browse our plasmid collection of popular lentiviral vectors .... . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....-free plasmid purification protocol. To minimize the immunogenic properties of the final vector preparation...

...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...all applications. Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but did...facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in... al. 2023 with permission.   Find Cas-PAGE plasmids here!   Zhang, Z., et al. (2023). Efficient engineering...inspired and informed this section.  Find mStayGold plasmids at Addgene here!   Ando, R., et al. (2023). StayGold...developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene...under CC-BY license.   Find the AAV.CAP-Mac plasmid here!   Chuapoco, M.R., Flytzanis, N.C., Goeden...

...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription... Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream... regions. Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating...preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI ...Janes 99283 pCW57.1-Luciferase Firefly TRE Lentiviral vector with dox- or tet-inducible luciferase expression...

...macrophages/monocytes using lentiviral expression vectors. I also used plasmids made and shared by others...Addgene, plasmids, and depositing (all while our US counterparts are still sleeping). Plasmid sharing ...have deposited their plasmids into the Addgene repository. More than 4,500 plasmids have already been deposited...in research, I developed several new plasmids, including: plasmids to study the dimerization of HLA B27... Years of Plasmid Sharing: Interested in more statistics from Addgene's 10 years of plasmid sharing? ...Check out our Top 10 Plasmid Technologies blog post Or look at our "10 Years of Plasmid Sharing" Infographic...emailed. And Addgene has been there to answer your plasmid questions. But until now, you’ve always had to ...

...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing...2 Pseudotyped Virus SARS-CoV-2 Spike Plasmids Accessory Plasmids Other Resources Mechanisms of SARS-CoV...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin...

...Development of a novel mouse glioma model using lentiviral vectors." Nature medicine 15.1 (2009): 110-116. PubMed...Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids Find Kits for Cancer Research  ...immunosurveillance have a look at our Immunology Research Plasmids and Resources. Studying the immune system and ...In contrast to using randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering...analyse the tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited... immunology resources. If you have a great new plasmid you’d like to share with the scientific community...

...one are in a pLVX vector background, so you can package them into lentiviral vectors if you need to. Additionally...going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6 construct is undergoing QC and... has been inundated with requests to share our plasmids with other researchers. We have been excited to...Gordon) have been quite busy shipping and sending plasmids to all who request them -- in locations all over...Facebook. Find more COVID-19 and coronavirus related plasmids and resources at Addgene* Many thanks to our...people, not just scientists! *If you are requesting plasmids for coronavirus or COVID-19 research, please email...

.... ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for ...Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids Blog: Inducible Promoters...tet-controlled gene of interest from one plasmid. A single vector approach may not have as much flexibility...Highlighted Plasmids Browse the tables below to find popular empty backbones, expression vectors for transactivators...transactivators, and vectors for other applications, or search our collection for all dox-regulated plasmids . Looking...Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... recent plasmid deposit please sign up here.      Listen to this episode of the Hot Plasmids podcast ... (ARG) plasmids to better monitor gene expression in vivo using ultrasound. These ARG plasmids contain...high-throughput manner. They designed the pXPR_053 lentiviral expression vector, which contains an sgRNA insert site...responses in C. elegans. Find the split BoTN/B plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed...potentially problematic for imaging. Find the ARG plasmids at Addgene. For more details on acoustic reporters...

... about Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages.... Viral vectors undergo DNA rearrangements. Depending on the composition of the viral vector and the ... one point or another. After amplifying a viral vector, collecting virus, and transducing target cells...the repeat sequences present in the LTR, the vector may undergo genomic rearrangements during replication...genomic rearrangements, try amplifying the viral vector with bacteria designed to minimize rearrangements...have low titers (which is unavoidable for some vectors) you can concentrate your virus by ultracentrifugation...antibiotic whose resistance gene is encoded in the viral vector. This antibiotic selection can be performed approximately...

.... At the time, adenoviral vectors were the most commonly used viral vectors for gene delivery, and they... better vector for ocular gene therapy: Adeno-Associated Virus (AAV) While other viral vectors such as...gene can be targeted to RPE cells using a lentiviral vector and a clinical trial is ongoing. Moving forward...Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral vectors ...AdV) and lentivirus (LV) are known to transduce retinal cells, AAV quickly became the vector of choice...technician in a lab using adeno-associated viral vectors (AAVs) to treat an inherited retinal degenerative...tolerate the presence of antigens (such as viral vectors) without triggering a destructive inflammatory ...

...the Addgene Blog Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene like...Once you've gotten to know what a plasmid is in general, it's time to take a look at some of its parts...origin of replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll...the promoter, which is necessary for getting the plasmid express our gene of interest. This element is responsible.... This means that the promoter present in your plasmid backbone must to be compatible with the type of... as RNAP II promoters is commonly employed in lentiviral and retroviral constructs and we will discuss...discuss these in a future post on viral vector parts. Promoter specificity Aside from choosing a promoter based...

...delivery of a plasmid. shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ...pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral packaging plasmids Web Resources...a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression...argonaute, please search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...

...empty gRNA plasmids . For more information on CRISPR plasmids and function, see our CRISPR plasmids resource...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled ...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in our repository...fluorescence. Morris Control Lentivirus Browse our list of available viruses based on plasmids in our repository ...

...available as pooled plasmid DNA. In addition, several libraries are offered as lentiviral preparations to ...are a little different: each is a collection of plasmids that share the same backbone and differ only in...hundreds more libraries to browse. Our Guide to Plasmid Pooled Libraries has tips on using these libraries...Additional resources on the Addgene blog Viral Vectors 101: Preparing Pooled Libraries Pooled Library ...Addgene.org Browse all pooled libraries Guide to Plasmid Pooled Libraries CRISPR Library Amplification Protocol...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available... Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell populations...populations without end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter... a) Cells are transfected with three different vectors and flow cytometry is used to sort populations ...available from Addgene, head over to our CRISPR Plasmids and Resources page. Here are some highlights from the...

...Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile ...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly used ... Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred to...Browse viral vector guides and plasmids available at Addgene and other related resources. Science...Viral Vectors Resources Viral vectors are popular tools for delivering genetic information to cells. These...These vectors rely on production plasmids that have been carefully optimized for both safety and efficiency...

...expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some...lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang and is described in Improved vectors and genome-...BRDN0000561167) (Plasmid #80034) and BRAF gRNAs (BRDN0001145345 and BRDN0001146266) (Plasmid #76479 and #76480...performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR... PubMed Central PMCID: PMC4486245.2. pXPR_011 (Plasmid #59702) was a gift from John Doench & David Root...Lines with Lentivirus Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production...of A549 cells transduced with Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI...

...roughly half the packaging limits of lentiviral and adenoviral vectors. While many transgene will fit within... overviews of three split vector strategies plus one additional split vector strategy for increasing the...homology directed repairs (HDR). Split AAV vector approaches Split vector approaches increase the size of the...copies of both vectors. Examples of use: Halbert et al., 2002 used an overlapping vector with 440 or 1000bp... hybrid vector, as measured by β-galactosidase activity, was 81% of that from a single vector, but significantly...ideal split vector system. Overall the efficiency of transgene expression from split vectors has been inconsistent..., Duan D (2008) A Hybrid Vector System Expands Adeno-associated Viral Vector Packaging Capacity in a Transgene-independent...

...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...ONE vector G-protein Optical (ONE-GO) biosensors (Janicot and Maziarz et al., 2024). Each plasmid expresses...biosensor in optimal proportions from a vector backbone that allows lentiviral packaging as a single payload (...selectivity. With 10 plasmids available as a kit, plus 21 optional accessory plasmids, the collection targets...precise plant breeding. Find dicot prime editing plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song... proteins for mammalian cell expression. These plasmids will be useful not only for future studies of ...proteins of interest too! Find CLIP, CLAP, and SNAP plasmids here! Smith, E.R., Turner, E.D., Abdelhamid, ...