We narrowed to 275 results for: mel

1. SciComm: Taking Science to Elementary and Middle Schools

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   ...does, and how one becomes a scientist. I gave a timeline of my science life, starting back to Mr. Kelley...engaging examples of how understanding genetics is so timely.” Genome engineering, and many other technologies...

2. The Importance of a Fun Workplace: Company Culture at Addgene

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   ... bit of Addgene history... As you may be aware, Melina Fan, her brother Ken Fan, and her husband Benjie...sometimes fun needs to be had in an orderly and timely fashion. 9. Ask for help My grandma used the old...

3. Plasmids 101: Broad Host Range Plasmids

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   ... Pseudomonas spp., Rhizobium leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas... Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum by. viciae, Rhodobacter sphaeroides...

4. Healthcare Consulting: A Door to the Business of Life Sciences

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   ...achievable tasks, and then executing on them in a timely fashion while allowing for creative solutions to...part of the profession, and that they can be ameliorated by thoughtful planning and engagement, leaving...

5. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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   ...using them grow. So, think beyond Drosophila melanogaster or Saccharomyces cerevisiae (both fine research... short legged lizards.   Zebra finches (Claudio Mello) These very cute birds learn their songs like humans...

6. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   ...partner Rho GTPase, leading to behaviors like lamellipodia or filopodia formation, cell contraction, or...they described tools to control formation of lamellipodia and contractile stress fibers, and recently ...

7. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   ....org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi... Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M,...

8. Harnessing Bacterial Toxins for Allelic Exchange

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   ...relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is a sucrase...Biotechniques 40.3 (2006): 355-364. PubMed PMID: 16568824. Hmelo, Laura R., et al. "Precision-engineering the Pseudomonas...

9. A History of Genome Engineering in Popular Culture

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   ...and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy...Darkness (2013) which is the remake of the space-melodrama Star Trek II: The Wrath of Khan (1982) combined...

10. Sequencing Options for CRISPR Genotyping

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    ...Provided 0.01% (variable) Sequence-level data, extremely sensitive Do not sequence all DSBs, may miss unpredicted...Comparison of Sanger sequencing, pyrosequencing, and melting curve analysis for the detection of KRAS mutations...

11. 9 tips for a successful postdoctoral experience

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    ...achieve your intended next career stage and create a timeline for achieving these goals. Planning is often more...not exist to answer the question or it might be extremely difficult for you to get the needed tools and/...

12. Antibodies 101: Introduction to Antibodies

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    ...the variable heavy chain fragment of heavy chain camelid antibodies (Hcab) (Arbabi-Ghahroudi et al., 2017...collection! References: Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective...

13. A Primer on Optogenetics: Introduction and Opsin Delivery

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    ...through promoter-directed expression alone is extremely limited. Therefore, highly expressing, generic... privately communicated to me as one of these extremely high expressing promoters). Conduct a time course...

14. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

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    ...individuals for green cards because of the cost, long timeline and uncertainty associated with filing a permanent....  Last but not least, NETWORK! Networking is extremely important for candidates during a job search and...

15. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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    ...designed for this purpose. This second area is extremely important and given the lack of attention to it...not entirely necessary but should be considered extremely useful, depending on design goals. Examples:  ...

16. CRISPR 101: Cytosine and Adenine Base Editors

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    ...., Slaymaker, I. M., Yen, J., Young, L. E., & Ciaramella, G. (2020). Directed evolution of adenine base...Schwaegerle, N. D., Peraro, L., Young, L., Lee, S.-J., Ciaramella, G., & Gaudelli, N. M. (2023). Improved cytosine...

17. 22 Hot Plasmid Technologies from 2014

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    ...Biotech. 2013. Dec 23.   Highly bright and extremely dim GFPs The availability of the three dimensional...unprecedentedly-high brightness, bfloGFPa1, and the other with extremely-dim brightness, bfloGFPc1. The plasmids used for...

18. Addgene's 20th Anniversary Party

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    ...even made an appearance during co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia...

19. Addgene is Stopping Use of X (formerly Twitter)

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    ...conflict with our values. For this reason, our timeline has accelerated. We significantly reduced posting...

20. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

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    ...some time out every once in a while – just read Melina Fan’s post on lunch. Why should our new blog be...