We narrowed to 570 results for: multi
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TypeBlog Post...indicated Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite® Plus 647 secondary antibodies. Antibody...antibody validation, and it is recommended to screen multiple candidate antibodies when possible. If cost is...techniques — and so it may be necessary to try multiple techniques. For example, we have found that some...potentially the need to study protein expression at multiple timepoints. When the target of interest is critical...challenging. Correlation with RNA or Proteomic data from multiple cell lines/ types with different expression Flow...with an assessment of labeling pattern between multiple antibody clones targeting the same protein (independent...be particularly useful when designing larger multicolour panels, where different manufacturers may have...
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CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...donor DNA. By using a multi-spacer CRISPR array, CRISPR-transposons can be multiplexed. Transcriptional repression...is a fast, multiplexable method that combines CRISPR and recombineering-based MAGE (Multiplex Automated...functions in S. pneumoniae and can be used to generate multiple mutations simultaneously (Jiang et al. 2013). ...mutations and 66% efficiency for small insertions. Multiplexing of two targets simultaneously is possible with...are its regulatable nature and its ability to multiplex repression. Using a Tet responsive promoter to...cassette, one can reliably knockdown expression of multiple target transcripts simultaneously. Marraffini ... system results in 80-90% RNA knockdown across multiple gRNAs per gene (Singh et al. 2016). Sarah Fortune's... -
27 Hot Plasmids from 2016
TypeBlog Post...improve the ease and convenience of assembling these multi-protein fusions or “polyproteams”, the Howarth lab...stimulate neurons. Recent studies have designed multi-component actuators that are sensitive to radio ...it comes to simultaneously modifying multiple loci (multiplexing). Currently, the only genome engineering... and basic biologists alike often need to test multiple expression systems before they achieve the appropriate...have greatly simplified the process of generating multiple E. coli gene expression plasmids with the Cross-disciplinary...library design, Moffat laboratory members conducted multiple screens in different human cell models and identified...method for bacterial genome engineering across multiple species Recent discoveries in the fields of genome... -
CRISPR Guide
TypeCollection...edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using dual...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single promoter...leveraged to achieve specific goals, such as targeting multiple genomic locations or enhancing specificity, efficiency...experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments involve...more than one gene. In these cases, delivering multiple gRNAs using a single plasmid ensures that all ...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...aureus Joung Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors...vectors. These systems can be used to express multiple gRNAs from a single plasmid with or without expression...expression of Cas9. Highlighted below are some multiplex gRNA vectors from our collection. Plasmids Expression...Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit Mammalian A system for constructing...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease... available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering from a single...Gate assembly. Gersbach MuLE System Mammalian MultiSite Gateway recombination-based cloning of complex... -
Fluorescent Protein Guide: Biosensors
TypeCollection...targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...sensor (cytosolic) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...or mitochondria Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...Campbell Voltage Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters... exceptional brightness, photostability, and multiplexing capabilities. bioRxiv. 2025 Jan 11:2024.12.16.628673...sensors for simultaneous monitoring of Zn(2)(+) in multiple cellular locations. PLoS One. 2012;7(11):e49371... -
Neurodegeneration Plasmid Collection
TypeCollection... Multisystem proteinopathy, ALS Nicolas Fawzi 98667 hnRNPA2_LC_WT_S329C HNRNPA2B1 His T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy... T7 Multisystem proteinopathy, ALS Nicolas Fawzi 157865 hnRNPA2_RRM1 HNRNPA2B1 His T7 Multisystem proteinopathy...Nicolas Fawzi 98657 hnRNPA2_LC_WT HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98658 hnRNPA2...hnRNPA2_LC_D290V HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98659 hnRNPA2_LC_D220V HNRNPA2B1... -
Deisseroth INTRSECT Collection
TypeCollection...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to...molecular payloads in target populations defined by multiple parameters....Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to...molecular payloads in target populations defined by multiple parameters. Figure 1: Examples of intersectional...definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations.... This approach has been broadly applied using multiple recombinase-expression strategies to define cellular...2014. Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods 11(7):763-72... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection... markers, respectively, or constructs in which multiple sgRNAs may be expressed from a single plasmid....non-deletion and deletion bands ( Figure 2 ). Consider multiplexing the “deletion” and “non-deletion” PCR primer...primer pairs in a single reaction. Optimize multiplexing in a polyclonal population ( i.e., bulk sorted cells...amplicons be easily resolved on an agarose gel for multiplexing. Screening CRISPR/Cas9 Clones for Deletions ...μl. This facilitates screening by allowing a multichannel pipette to be used for the remainder of the ...in each well) to a 96-well PCR plate using a multichannel pipette. Centrifuge PCR plate at 400 x g for...agar plates with the relevant antibiotic. Select multiple colonies, mini-prep each one, and subject each... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...interest. They may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible promoter, and...frequently used in molecular biology to isolate, multiply, or express the insert they carry in the target...CaMV35S, RPS5A, Ubiquitin pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea...RFP - A zebrafish insulin promoter vector with multiple cloning sites Browse our full Fluorescent ...bacterial gene clusters S. cerevisiae plasmids for multiple marker selection TRP1 Yeast pGBKCg - Gateway compatible... -
Plasmids for Stem Cell Research
TypeCollection...body. Multipotent stem cells, such as adult (or somatic) stem cells, can differentiate into multiple cell... Stem cells are classified as pluripotent or multipotent, based on their ability to generate different...related cell lineages. Pluripotent embryonic and multipotent adult stem cells have been studied for over 30... -
CRISPR Plasmids for Genomic Visualization
TypeCollection...for different genomic loci, capable of detecting multiple genomic loci, and compatible with live cell imaging...visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive...genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting... -
New England Biolabs Cell-Imaging Plasmid Collection
TypeCollection...community. Key features: Allows for visualization of multiple proteins simultaneously with dual labeling when...proteins Single constructs are compatible with multiple applications (different fluorophores, biotin, ...George N, Krumm H, Johnsson K, Johnsson N. 2005. Multicolor imaging of cell surface proteins. J Am Chem Soc... -
mTOR Pathway
TypeCollection...p53 also promotes aberrant mTORC1 activity, and multiple familial cancers are caused by inactivating mutations...for the gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each...available plasmids for the gene. For components with multiple isoforms or subunits, individual links to each... -
Validated gRNA Sequences
TypeCollection...cut S. pyogenes 26028531 Huangfu OCT4 H. sapiens multiple, see article 69537 activate S. pyogenes 26352799... pyogenes 25336740 Christiaen EGFP A. victoria multiple, see article 60071 dCas9-FokI S. pyogenes 24770325...23817069 Calarco Kras, p53, and Lkb1 M. musculus multiple, see article 60224 cut S. pyogenes 25263330 Zhang.... pyogenes 23929340 Kamoun PDS1 N. benthamiana multiple, see article 49772 cut S. pyogenes 24112467 Kamoun... -
Zebrafish Plasmid Collection
TypeCollection...Lab Lineage Tracing Cell lineage tracing in multicellular systems typically relies on genetic markers,...Development and Regeneration - Periklis Pantazis Lab Multibow: digital spectral barcodes for cell tracing - ...Lab Zebrabow - Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism... -
Lentivirus Plasmids
TypeCollection...particle (a virion) are generally divided among multiple plasmids. Some plasmids (typically called packaging...Tet-inducible lentivirus for ORF expression, multicistronic design enables tracking of viral transduction...plasmid (pGIPz based) for inducible knockdown, multicistronic design enables tracking of viral transduction... -
Cre-lox system
TypeCollection...neurons with multiple distinct colors Mammalian Sanes See article Multibow constructs Multibow uses a binary... to facilitate Cas9 mediated genome editing in multiple tissues. FLP/FRT: Alternative recombinase system... -
Fluorescent Protein Guide: In Vivo Imaging
TypeCollection... effective brightness and allow for single or multicolor imaging. Photo-activatable iRFPs can be ‘turned...empty backbone plasmids are ideal for single or multicolor in vivo imaging. Plasmids for In Vivo Imaging... -
Adenovirus Plasmids
TypeCollection...pShuttle-CMV-F2A-T2A-Venus Shuttle For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for...contain modular inserts for seamless assembly of multiple mutations, alterations, or transgene insertions...