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...Protein Imaging Choosing Your Fluorescent Protein for Multi-Color Imaging Catch Up on Your GFP Background Resources...state (“quenching”). The photoswitch can occur multiple times by activating with two distinct wavelengths...experiment even begins! If you are performing multicolor experiments, you will want to be sure that the...relies heavily on the same fluorophore being imaged multiple times in order to reconstruct an image. The best...Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy...Collection Find Photoactivatable FPs for Single and Multicolor in vivo Imaging Browse All Fluorescent Protein...

... indels Cytosine transversion editors rely on a multi-step cellular repair pathway that is known for sometimes...that site is filled depends on cellular factors. Multiple labs tried to identify beneficial factors to include...Several of these proteins had additive effects when multiple were included in the CGBE. They also developed...

...indicated Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite® Plus 647 secondary antibodies. Antibody...antibody validation, and it is recommended to screen multiple candidate antibodies when possible. If cost is...techniques — and so it may be necessary to try multiple techniques. For example, we have found that some...potentially the need to study protein expression at multiple timepoints. When the target of interest is critical...challenging. Correlation with RNA or Proteomic data from multiple cell lines/ types with different expression Flow...with an assessment of labeling pattern between multiple antibody clones targeting the same protein (independent...be particularly useful when designing larger multicolour panels, where different manufacturers may have...

...donor DNA. By using a multi-spacer CRISPR array, CRISPR-transposons can be multiplexed. Transcriptional repression...is a fast, multiplexable method that combines CRISPR and recombineering-based MAGE (Multiplex Automated...functions in S. pneumoniae and can be used to generate multiple mutations simultaneously (Jiang et al. 2013). ...mutations and 66% efficiency for small insertions. Multiplexing of two targets simultaneously is possible with...are its regulatable nature and its ability to multiplex repression. Using a Tet responsive promoter to...cassette, one can reliably knockdown expression of multiple target transcripts simultaneously. Marraffini ... system results in 80-90% RNA knockdown across multiple gRNAs per gene (Singh et al. 2016). Sarah Fortune's...

...improve the ease and convenience of assembling these multi-protein fusions or “polyproteams”, the Howarth lab...stimulate neurons. Recent studies have designed multi-component actuators that are sensitive to radio ...it comes to simultaneously modifying multiple loci (multiplexing). Currently, the only genome engineering... and basic biologists alike often need to test multiple expression systems before they achieve the appropriate...have greatly simplified the process of generating multiple E. coli gene expression plasmids with the Cross-disciplinary...library design, Moffat laboratory members conducted multiple screens in different human cell models and identified...method for bacterial genome engineering across multiple species Recent discoveries in the fields of genome...

...edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using dual...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single promoter...leveraged to achieve specific goals, such as targeting multiple genomic locations or enhancing specificity, efficiency...Figure 2: Overview of the NHEJ repair mechanism. Multiplex Genome Engineering Many CRISPR experiments involve...more than one gene. In these cases, delivering multiple gRNAs using a single plasmid ensures that all ...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell...

...aureus Joung Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors...vectors. These systems can be used to express multiple gRNAs from a single plasmid with or without expression...expression of Cas9. Highlighted below are some multiplex gRNA vectors from our collection. Plasmids Expression...Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit Mammalian A system for constructing...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease... available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering from a single...Gate assembly. Gersbach MuLE System Mammalian MultiSite Gateway recombination-based cloning of complex...

...targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...sensor (cytosolic) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...or mitochondria Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...Campbell Voltage Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters... exceptional brightness, photostability, and multiplexing capabilities. bioRxiv. 2025 Jan 11:2024.12.16.628673...sensors for simultaneous monitoring of Zn 2+ in multiple cellular locations. PLoS One. 2012;7(11):e49371...

... Multisystem proteinopathy, ALS Nicolas Fawzi 98667 hnRNPA2_LC_WT_S329C HNRNPA2B1 His T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy... T7 Multisystem proteinopathy, ALS Nicolas Fawzi 157865 hnRNPA2_RRM1 HNRNPA2B1 His T7 Multisystem proteinopathy...Nicolas Fawzi 98657 hnRNPA2_LC_WT HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98658 hnRNPA2...hnRNPA2_LC_D290V HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98659 hnRNPA2_LC_D220V HNRNPA2B1...

...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to...molecular payloads in target populations defined by multiple parameters....Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to...molecular payloads in target populations defined by multiple parameters. Figure 1: Examples of intersectional...definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations.... This approach has been broadly applied using multiple recombinase-expression strategies to define cellular...2014. Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods 11(7):763-72...

...interest. They may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible promoter, and...frequently used in molecular biology to isolate, multiply, or express the insert they carry in the target...CaMV35S, RPS5A, Ubiquitin pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea...RFP - A zebrafish insulin promoter vector with multiple cloning sites Browse our full Fluorescent ...bacterial gene clusters S. cerevisiae plasmids for multiple marker selection TRP1 Yeast pGBKCg - Gateway compatible...

...body. Multipotent stem cells, such as adult (or somatic) stem cells, can differentiate into multiple cell... Stem cells are classified as pluripotent or multipotent, based on their ability to generate different...related cell lineages. Pluripotent embryonic and multipotent adult stem cells have been studied for over 30...

...for different genomic loci, capable of detecting multiple genomic loci, and compatible with live cell imaging...visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive...genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting...

...community. Key features: Allows for visualization of multiple proteins simultaneously with dual labeling when...proteins Single constructs are compatible with multiple applications (different fluorophores, biotin, ...George N, Krumm H, Johnsson K, Johnsson N. 2005. Multicolor imaging of cell surface proteins. J Am Chem Soc...

...p53 also promotes aberrant mTORC1 activity, and multiple familial cancers are caused by inactivating mutations...for the gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each...available plasmids for the gene. For components with multiple isoforms or subunits, individual links to each...

...Lab Lineage Tracing Cell lineage tracing in multicellular systems typically relies on genetic markers,...Development and Regeneration - Periklis Pantazis Lab Multibow: digital spectral barcodes for cell tracing - ...Lab Zebrabow - Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism...

... standards Thaler et al. (2005) Quantitative multiphoton spectral imaging and its use for measuring resonance...Y67C) constructs for measuring energy transfer to multiple acceptors Koushik et al. (2009) Anomalous surplus...surplus energy transfer observed with multiple FRET acceptors mTurquoise2-mVenus FRET calibration standards...

...infectious viral particle (a virion) are divided among multiple plasmids: Transfer plasmid — contains transgene...Tet-inducible lentivirus for ORF expression, multicistronic design enables tracking of viral transduction...plasmid (pGIPz based) for inducible knockdown, multicistronic design enables tracking of viral transduction...

...pShuttle-CMV-F2A-T2A-Venus For production of viruses with multicistronic expression and a Venus tag Wang 50959 ShuttleMCP...-CMV-F2A-T2A For production of viruses with multicistronic expression Wang Return to top Adenoviral Plasmids...

...subsets of barcode proteins to label and detect multiple injection sites in the same tissue, as well as...generates unique combinations of protein bits. B) Multiplex imaging of barcodes with expansion microscopy ...