...efficiency.  Finding recombinant psychrophilic enzymes for the host The expression of recombinant proteins for...well under suboptimal conditions. This means recombinant proteins can aggregate or precipitate out as ...would allow the stable folding and expression of recombinant psychrophilic proteins. As the figure below shows... as engineering and regulating psychrophilic recombinant proteins.  To infinity, and beyond! Keeping ...

....org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant antibody plasmid...diabodies to be used for assembling protein nanostructures. Diabodies readily co-crystalize, a major advantage...antibodies (sdAbs), sold under the commercial name nanobodies, are derived from a camelid antibody lacking ...10.1016/j.ymeth.2018.09.005 Muyldermans S. (2013). Nanobodies: natural single-domain antibodies. Annu Rev Biochem...

...purity of DNA samples. These days, many labs have a NanoDrop, which is a very small spectrophotometer that ...little as 1μl. Regardless of whether you have a NanoDrop, follow the manufacturer's instructions for the...inherent to the machine and your solvent. If using a NanoDrop to measure your samples, place 1-2µL of mini-prepped... Keep in mind that despite the accuracy of the NanoDrop, if two consecutive samples have significantly... between them has affected the accuracy of the NanoDrop. It is a good idea to re-zero any spectrophotometer...

...would seem to save time and money but cells lose provenance (this is the history of the cell line consisting...contamination with HeLa being the most frequent contaminant, closely followed by T24 bladder cancer cells... Public Domain. The basics of good cell line maintenance The key is good recordkeeping, you need to have...journals strictly require evidence of cell line provenance and identity. The Nature Publishing Group recently... cell line maintenance...

...coli strains are not usually well suited for recombinant protein expression. Many challenges can arise...coli. We will review the potential pitfalls of recombinant protein expression and some of the most popular...or are enzymatically active. For this reason, recombinant proteins are typically expressed in E. coli engineered..., which reduces proteolysis of the expressed recombinant proteins. lon protease: Strains where this is...temperature, time, and media conditions. Many recombinant proteins express better at 30°C or room-temperature...

...BRET= Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer    Interesting...now well-known FRET, aka Fluorescence (Förster) Resonance Energy Transfer, technique which allows the detection...have any cross-talk but you also don’t have any resonance transfer. You have to find a balance between FRET....201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET) microscopy imaging of live...

...variants of their Nano-lantern technology, first developed in 2012. The new cyan and orange Nano-lantern fusions...multi-color imaging. The Nano-lanterns rely on the principle of bioluminescence resonance energy transfer (BRET...and pcDNA3.1+-pHlash, that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson...two improved Light Inducible Dimers (iLID), iLID nano and iLID micro, which differ from each other by ...light/dark affinity range (130nM to 4.7µM for iLID nano and 800nM to 47µM for iLID micro; Figure 1). With... al. Cell reports. 2015. PubMed PMID: 26456817. Nano-lanterns for multicolor luminescence imaging  Fluorescent...their 2015 PNAS manuscript, Takai et al validate the Nano-lantern approach as a tool for monitoring multi-...

...techniques. For example, restriction mapping led to recombinant DNA technologies that are now commonplace in ...sequencing world, one up and coming innovation is nanopore-based DNA sequencing which reads the nucleotides...nucleotides (8). Compared to older technologies nanopore-based DNA sequencing machines are relatively small...have high error rates (10). It is projected that nanopore-based sequencing technologies could one day sequence...PubMed PMID: 1925562. 10. Feng, Yanxiao, et al. "Nanopore-based fourth-generation DNA sequencing technology...

...focus on defining these resources and not on the financial aspects. Money often carries an emotional weight...administrative services, managers, and techs’ financial books.  To find personnel salary ranges, go to...specific aims. 2. Then make decisions based on financial values. All too often, we see early investigators...save you money in the short term. However, the financial risks associated with old equipment, like service.... Always focus on the science When negotiating finances, instead of solely discussing numerical values...

...brain samples from the James Trimmer Lab, using recombinant antibodies with different isotypes. (A) Neocortex... affinity reagents like fluorescently-labeled nanobodies or scFvs. Many, many options for fluorescently.... (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...Beuschel, R. T., Kabat, J., Croteau, J., Davis, J., Hernandez, J. M., & Germain, R. N. (2020). IBEX: A versatile...Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for...

...fluorescently tagged nanocellulose for nanotoxicological studies. Environmental Science: Nano, 6(5), 1516–1526...isothiocyanate (FITC) can be stably combined with nanoparticles, maintaining intensity at ~85% of its original...specificity to ensure accurate tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes ...

...contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique in modern ...only excites fluorophores within a few hundred nanometers of the glass/water interface. Super resolution...only excites fluorophores within a few hundred nanometers of the coverslip (4). Essentially, an optical...10 fold increase in resolution, down to 10s of nanometers. Thick dynamic samples Ex: 3D cell cultures, ...KI, Sahl SJ, Hell SW. Lens-based fluorescence nanoscopy. Quarterly reviews of biophysics 48, 178-243 (...

...protocol is composed of steps that separate out contaminants from an impure AAV preparation. The 15% iodixanol...macromolecules. The 40% and 25% steps are used to remove contaminants with lower densities, including empty capsids.... Image adapted from Zolotukhin, S., et al. "Recombinant adeno-associated virus purification using novel...Left image adapted from Zolotukhin, S., et al. "Recombinant adeno-associated virus purification using novel...by silver stain. Note the increased number of contaminants in each fraction. * AAV capsid proteins VP1,...

...and the ITRs are retained with the gene in a recombinant AAV (rAAV) vector (Figure 1). Everything else...outside the ITRs gets left behind.    Figure 1: Recombinant AAV vector with ITRs that flank the gene cassette...plasmid DNA with mutated ITR sequences. Using recombinant-deficient bacterial strains such as NEB Stable...individual reads allows for identification of non-dominant ITR sequences.  Restriction digests with the enzymes...

...CRISPR/Cas9 could be used to edit several autosomal-dominant mutations that have been connected to the early...Chinese Academy of Sciences found that mice with a dominant mutation in a gene that causes cataracts could... chose to explore its potential in mice with a dominant cataract disorder caused by a single copy of a...applications are also possible through the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR...

...auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S. in accounting... on average 20 PhD’s a year and we have a very dominant presence in the biotech sector in both patent ...effectiveness of the Plan's risk management, control, and governance processes. Prior to Tufts Health Plan, Carl worked...

... The binding of the GFP nanobody to its target stabilizes the TurboID-nanobody protein and allows TurboID...TurboID fused to a conditionally stabilized GFP-nanobody rather than a specific protein of interest. This...GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The modularity of the system...

...others you’ll encounter include monoclonal and recombinant antibodies). By the end, you’ll have a better...polyclonal antibody, or select a monoclonal or recombinant antibody instead. Otherwise, you might experience...of background noise compared to monoclonal or recombinant antibodies. This is because the polyclonal antibody...antibodies Learn about the advantages of using recombinant plasmid-based antibodies Resources on Addgene.org...

...might not be the right choice. I spoke with our Financial Operations Specialist, Lucy Hammer, about why ...response to this question came from Lucy Hammer, our Financial Operations Specialist. She said, “I’ve found the...came to Addgene with a goal to learn nonprofit finance, and while I've done that, my big takeaway has ...conversation master knows that although her focus is finance, it is just as important to bring love and fun ...

...neural connectivity (Nassi et al. 2015). The recombinant viruses typically used in neuroscience research...transmitted transsynaptically). This is because the recombinant forms of these viruses typically used in research...characteristics of a G-deficient rabies virus recombinant: an in vitro and in vivo study. J Gen Virol. ...Osakada F, Callaway EM. Design and generation of recombinant rabies virus vectors. Nat Protoc. 2013;8:1583...