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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 304 results for: pET

Showing: 81 - 100 of 304 results

  1. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...Professional Development Strategy to increase your competencies and skills in the grants profession. Your professional... and the candidate’s knowledge of the 8 core competencies through a multiple choice exam.  The bottom ...grant professionals and to build your skills and competencies. Becoming a grant professional is rewarding ...

  2. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...decreasing the probability of creating replication-competent lentivirus (RCL) in your target cells.  Q4: How...targeted. Q6: Are transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer...are: 1) the potential generation of replication competent virus [usually HIV-1]; and 2) the potential for...

  3. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...your experiments. Don’t dive directly into the pipetting! MoClo nomenclature and structure can be quite...To combat this, you can increase the volume of competent cells you plate after each transformation and/...high-throughput, we recommend transforming into chemically competent cells in a 96-well PCR plate. Always confirm your...

  4. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...sample is immediately ready to transform into competent cells. The master mix of enzymes can be purchased...master mix for 1 hour, and then transform into competent cells. The success rate of this reaction is typically...terminator sequence), since this would directly compete with the required single-stranded annealing and...

  5. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...restriction-deficient; good strain for cloning repetitive DNA. endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB...lacY1 proA2 rpsL20 xyl-5 leu mtl-1 Stbl4   Electrocompetent cells for cloning and storage of unstable ...XL10 Gold Tetracycline and Chloramphenicol High competency cloning and propagation of large plasmids, ligated...

  6. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...lots of hustle and bustle, but the lab was very competitive and not very communal. That didn’t suit my collaborative... of effort, intimidating use of discipline or competence procedures Threats to personal standing – including...atmosphere makes you successful. Collaborative Competitive Papers have multiple authors One author plus...

  8. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...done in reverse. Assuming you want to become competitive for a Group Leader position, what will you need... you get more publications, you will be more competitive for fellowships. If you explore your system more...job search. Therefore, you must balance these competing goals. Knowing when to follow a lead and when ...

  9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ... to poor lab techniques. These include reusing pipettes for multiple cell lines rather than using disposable...aerosols that are generated (while talking or pipetting) during working in the laminar airflow are also... are tightly packed with seals. Use disposable pipettes to avoid cross-contamination. Make sure you keep...

  11. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...favorite twitter #agarart accounts, Petri dish Picasso (@PetriPicasso) came together and delivered very ...

  12. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ... 1. Lab B needs a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave...species for the challenge, but one species always outcompeted the other species.  When he found the chromoprotein...

  13. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...learn more about. Our post on “Making Your Own Competent Cells” has been extremely popular, generating ...Morgan Save Time and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter...

  14. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...dire:  The current system is unsustainable, hypercompetition is corroding values, we are shifting away ...such as the National Postdoc Association Core Competencies and the Science Careers’ myIDP). Dr. Galit Lahav...

  18. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...overnight. 4. Transform TOPO Cloning Reaction into Competent Cells: You can use your standard lab protocol ...transforming your reaction into chemically or electro-competent E. coli - excess salt causes arcing during electroporation...

  19. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...filing cabinets, a rickety old incubator, a few pipettes, and some home-made equipment. It was humble in...year with a computer science degree and had zero pipetting experience. However, after a few months of dedicated...

  20. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...during transformation First, use enough DNA and competent bacteria to ensure all components of the library...library are represented. Typically, Addgene uses electrocompetent Stbl4 cells when performing amplifications...
Showing: 81 - 100 of 304 results