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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 147 results for: pam

Showing: 81 - 100 of 147 results

  2. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...site-directed mutagenesis can be used to mutate the PAM sequence (an NGG sequence critical for Cas9 cleavage...

  3. Plan Your Experiment

    Type
    Collection
    ... no PAM site close to your desired edit site. Consider alternative Cas9 enzymes with different PAM sequences...activity within a very narrow window proximal to the PAM sequence Base Edit Prime Edit Generate targeted insertions...sequences or Cas9 enzymes with flexible PAM sites . Base Editing : Target the site of your desired edit...their functions within a limited window from the PAM sequence, and base editor variants often have different...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... and PAM happen to fall on the bottom (Crick) strand while sgRNA-B’s protospacer sequence and PAM fall... “NGG” sequence (“protospacer adjacent motif” or PAM) at the genomic recognition site. NOTE: Figure 1 ...independent of orientation of the protospacer sequence/PAM relative to the top or bottom strand. Determine the...

  8. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...sequence identity to the spacer sequence and contain a PAM sequence just downstream of the sequence to be cleaved...

  9. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In...

  10. CRISPR Plasmids - Parasites

    Type
    Collection
    ...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell...

  12. Zhang Lab CRISPR Page

    Type
    Collection
    ...and need to be followed on the 3' end by a 3bp NGG PAM sequence. We have found that adding an additional...and need to be followed on the 3' end by a 3bp NGG PAM sequence. 3. SpCas9 alone: This plasmid contains ...need to be followed on the 3' end by NGRRT or NGRRN PAM sequences. We have found that adding an additional...

  18. CRISPR Plasmids - Plants

    Type
    Collection
    ...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell...

  19. Validated gRNA Sequences

    Type
    Collection
    ... may only be compatible with a single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must...used with targets that are upstream of a 5' NGG 3' PAM sequence). Which CRISPR application is this gRNA ...
Showing: 81 - 100 of 147 results