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We narrowed to 290 results for: poly

Showing: 81 - 100 of 290 results

  2. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...high-fidelity polymerase to ensure accuracy. In this case, you would want a low-fidelity polymerase, like Taq...forcing sequence diversity using error-prone polymerases, you force sequence diversity using a pool of...

  3. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this range needs...this starting point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase... time Not enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein...

  5. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... bacterial genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted... into three distinct types and subtypes using polythetic classification based on evolutionary similarity...

  6. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...one epitope. (It can sometimes be an issue with polyclonal antibodies, so it's good to keep epitope availability...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

  9. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make...

  10. 5 Great Apps for Lab Life

    Type
    Blog Post
    ... depending on the primer sequence and the NEB polymerase used. Overall, while much of the information ...allow you to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, ...

  11. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset and ligate mismatched...primer design based on fragment sequences and the polymerase used in the amplification. This tool allows instant...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ..., NIH3T3, CHO, 293HEK, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation 100...hygB HeLa, NIH3T3, CHO, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation. ...

  13. Antibodies 101: ChIP

    Type
    Blog Post
    ...incubate the sample with your antibody.  Both polyclonal and monoclonal antibodies can work well in ChIP...Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

  14. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Cassettes transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9...Cassettes transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9  ...

  15. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...The 5’ LTR and 3’ LTR, respectively, act as RNA polymerase II promoter and terminator regions and are slightly...replication, with E2B being the primary viral polymerase. E4/E1A activate transcription of other relevant...

  17. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...). mAbs and rAbs have higher homogeneity than polyclonal antibodies because of their immunoglobin component...circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially unlimited ...

  18. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ... the reverse transcriptase, protease, and Bst polymerase constructs from their assay to Addgene.  Drew...expressing MMLV reverse transcriptase and Taq polymerase that can be used in a one-step RT-qPCR reaction...Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020...

  19. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...In addition the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), ...centrifugation on a sucrose gradient, spin columns, and polyethylene glycol (PEG) precipitation. All of these methods...

  20. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...and Bester 2021). For example, inserting a polyadenylation signal downstream of the transcription start...fragment. (D) Knock-in: insertion of a synthetic polyadenylation (spA) signal. Figure and caption adapted from...
Showing: 81 - 100 of 290 results