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Addgene

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Showing: 81 - 100 of 110 results

  10. Antibody Guide

    Type
    Guide
    ...long-term storage they should be stored at -20 °C in aliquots of at least 10 µL. Antibodies cannot tolerate ... some cases, it may be preferable to buy small aliquots of many different antibodies and do batch validation...

  11. Molecular Biology Reference

    Type
    Guide
    ...recommends making 1000X stock solutions and storing aliquots at -20°C. To use, dilute your antibiotic into ...

  12. General Transfection

    Type
    Protocol
    ... through a 0.22 µm filter. Aliquot 50–100 µL and store at -20 °C. Aliquots can be thawed and stored at...at 4 °C prior to use. Thawed aliquots should be discarded after 1–2 months. 1 mg/mL polyethylenimine, ...Filter the solution through a 0.22 µm membrane. Aliquot 500–1000 µL into sterile tubes. Store the tubes...

  13. AAV Production in HEK293 Cells

    Type
    Protocol
    ...1.0 μg/μL = 468.1 μL Aliquot 100 mL of OptiMEM into a sterile 250 mL bottle. Aliquot 774 mL of DMEM + 2%...Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into sterile tubes. Store the tubes...recommended or the solution may separate into phases. Aliquot and store at 4 °C. Cell Lysis Buffer : 50 mM Tris...

  14. CRISPR Library Amplification

    Type
    Protocol
    ... 200 µL micropipette tips in a -20 °C freezer. Aliquot 3 mL SOC into each of four 14 mL Vented Falcon .... Procedure Day 1 Add 200 ng DNA to each 50 µL aliquot of thawed Endura Duos on ice. Flick gently to mix...Micropulser Ec1 0.1 cm cuvette, 1.8 kV, 1 pulse. Aliquot 25 µL DNA-Endura into pre-chilled cuvette. Pulse...containing 3 mL SOC. Repeat for each of the 25 µl aliquots of cell/DNA mixture used (8 in the described protocol...

  15. Fluorescence Titering Assay

    Type
    Protocol
    ... complete. Mix well by pipetting or inverting. Aliquot 2 mL of cell suspension into each well of the 6...cycles of freeze-thaw. If you are freezing and aliquoting virus, it is recommended that you titer from ...using frozen virus, rapidly thaw the lentiviral aliquot on ice prior to use. Prepare dilutions of the lentivirus...

  16. Colony Formation Titering Assay

    Type
    Protocol
    ...cycles of freeze-thaw. If you are freezing and aliquoting virus, it is recommended that you titer from ...freeze-thaw. If using frozen virus, thaw the lentiviral aliquot on ice prior to use. Prepare 10-fold serial dilutions...polybrene. Mix well by pipetting or inverting. Aliquot 1.35 mL of the cell suspension into each well of...

  17. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...through a sterile 0.22 µm filter. Prepare 10 mL aliquots and store the solution at -20 °C. 1 mg/mL PEI-...the solution through a 0.22 µm filter. Prepare aliquots and store at -20 °C until use. BCD TFX 1000 mL...sterilize through a 0.2 µm PES syringe filter. Aliquot and freeze upright at -20 °C. Procedure Section...

  19. Lentivirus Production

    Type
    Protocol
    ...Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into sterile tubes. Store the tubes...stored at 4 °C for several hours but should be aliquoted and snap frozen in liquid nitrogen and stored ...

  20. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...polybrene into 20 mL media. Thaw the lentiviral aliquot on ice prior to use. Prepare a range of dilutions.... Mix well by pipetting or inverting the tube. Aliquot 1 mL of cell suspension (i.e., 50,000 cells) into...
Showing: 81 - 100 of 110 results