We narrowed to 370 results for: reporter

... available with T-Sapphire, EGFP, or mScarlet reporters, so you can choose a color compatible with the...and AAV11 tropism and applications. A) AAV6 is reported to have broad tissue tropism, including skeletal...hematopoietic cells, and immune cells. B) AAV11 is reported to target skeletal muscle, kidney, spleen, lung...the Cre recombinase payload activates a floxed reporter gene (genetically encoded or delivered separately...

...are removed and replaced by a single (non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked...necessary to direct minigenome replication and reporter gene transcription by the EBOV polymerase (reviewed...the minigenome as a template to transcribe the reporter gene (e.g. firefly luciferase). The polymerase...start signal and initiates transcription of the reporter gene. The polymerase will elongate the mRNA until...

... in sample treatment across methods can affect reported titer by either method so to ensure equal animal...AAV8. Very recently an engineered AAV5 capsid was reported for specific and potent liver transduction. While...marmosets each. Brain potency for each individual is reported in Figure 4B (see left for image and link for ...

..., Bernach plans to use a suite of fluorescent reporters. Bacteria containing unstable fluorescent proteins...colonizer, labelled with a constitutively fluorescent reporter can also be used to track how one population responds...using TIMER and an unstable fluorescent protein reporter under the control of promoters that control cell... the TIMER growth rate bioreporter. During protein maturation, the bioreporter spontaneously changes from...artificial leaf, Bernach will use the CUSPER bioreporter such that fluorescent proteins expression is ...

...not used for general plasmid selection. Instead, reporter genes are often employed to easily monitor transfection...expression levels in the cells. Ideally, the chosen reporter is unique to the cell, is expressed from the plasmid...assess transfection success. GFP is often used as a reporter and we will be covering this ever-versatile fluorophore...

...primary antibody required. Reporters There are two main classes of reporters: chemiluminescence enzymes...you need a specific conjugate, and changing your reporter molecule requires either re-conjugating or re-...-purchasing your primary antibody with the new reporter. Finally, each new conjugate will have to be tested...film is only compatible with chemiluminescence reporters and requires access to a darkroom and a developer... time; higher background Chemiluminescence reporter Smaller dynamic range; no multiplexing Protein-based... time; higher background Chemiluminescence reporter Smaller dynamic range; no multiplexing Wet...

... targeting astroglia. It has additionally been reported to have tropism for liver, vascular endothelial... muscle inflammation and degeneration has been reported (Muraine et al., 2020). AAV8 AAV8 has high transduction... the CNS (Han et al., 2023), and has also been reported to have tropism for liver and kidney cells (Issa...Use Addgene’s AAV Data Hub to find experimental reports on commonly used vector cargo designs and administration...cells utilizing various AAV serotypes. Scientific Reports, 15(1), 20927. https://doi.org/10.1038/s41598-025...

...only 46% could be reproduced. In a recent Nature report from researchers at Amgen, it was stated that only...through all saved data and automated writing of reports. To be able to understand the situation in depth...keep paper records as well as digital, or for reporting on a project – printing your data out can be required... you might need share data with them including reports and protocols. To facilitate this, some ELNs allow...

...Russell and Hirata from the University of Washington reported a surprising finding - when delivered via AAV ...take up ~6.5 kB if delivered in a single AAV. They report roughly 40% repair efficiency in the K562 leukemia...AAV vector cures haemophilia B mice." Scientific Reports 7.1 (2017): 4159. PubMed PMID: 28646206. PubMed... gene cassettes using AAV donor vectors." Cell reports 20.3 (2017): 750-756. PubMed PMID: 28723575. PubMed...

...in the same lane, and the resulting fraction is reported. Figure 2: Normalization with loading controls...equally strong, so the normalized value simply reports which POI band is stronger. The right gel has exactly...abundance on your membrane, which would be directly reported by band intensity. Researchers use later steps...perturbed by the experiment Unfortunately, multiple reports suggest that common housekeeping proteins are influenced...

...gene disruption in M. abscessus  Bioluminescent reporter of mechanical forces   Epitope tag antibody ...Western Blot for Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke...doi.org/10.1128/jb.00335-23.  Bioluminescent reporting of mechanical forces with PILATeS By Emily P. ... reaction (Figure 5). The researchers used the reporter to visualize biologically relevant forces at focal...

...Neuroscience Community blog to read reports from bloggers at the meeting – my report is currently in the works....

... also lists several luciferase and fluorescent reporter plasmids that have been used for measuring viral...acids SARS-CoV-2 main protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase...

...microbiome by using engineered genetic tools and reporter microbes. Let’s take a look at a few of these ...is a ribosome binding site (RBS) followed by a reporter gene. The hairpin blocks translation by sequestering... reveals the RBS and allows translation of the reporter gene resulting in GFP signal. With the addition...

...step. Scorpion chemistry in particular has been reported to work well in the Lightcycler while being relatively...Yes Agarose gel Sunrise primers   Low Reporter and quencher on hairpin structure included in ...much as sixfold variation in expression levels reported (Biassoni, 2014), so you’ll want to ensure that...conceptualize. Just remember to switch them back before reporting the data!   Statistical analysis  Getting...frown upon statistical laxness in all its forms. Reporting your data  Once you’ve done the work to produce...(Bustin, 2009) to ensure you’re recording and reporting the right information so others can reproduce ...

... an ERK kinase translocation reporter with a mRuby2 fluorescent reporter (ERK KTR-mRuby2). The ERK KTR...Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins...

...by reducing the distance between the target and reporter. They have fewer non-specific binding opportunities...it either needs to be directly conjugated to a reporter or you need a light-chain specific conjugated ...fairly easy to find these in a Fab fragment format. Reporter-conjugated anti-IgG Fab fragments make good secondaries...

...of interest and co-expressing a cytosolic ZtSH2 reporter, they observe fluorescence localizes to the plasma...And orthogonal sensors with different ITAM/SH2 reporter pairs allow multiplexed measurement of different...10.1101/2022.08.13.503850.  iGECInano NIR calcium reporter Near-infrared (NIR) fluorescent proteins promise...photochemical characteristics than previous NIR calcium reporters iGECI and NIR-GECO2G, and its small size (~1500...

...library by in vivo functional screen." Scientific reports 5 (2015). BIOFAB plasmid set 3000 Series Confronted...strengths centered within GFP/RFP bicistronic reporters characterized in Cambray et al., 2013. These terminators... an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31-37. pCri...fusions. Some applications of PUF protein fusions reported in the literature include RNA detection, RNA cleavage...

...responsible for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher...he lived! His hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. ...