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We narrowed to 135 results for: sacs

Showing: 81 - 100 of 135 results

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Plasmids 101: Cre-lox

Type

Blog Post

...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another...
Overcoming the Challenges of Lentiviral Production

Type

Blog Post

...systems such that it no longer requires the viral transactivator, Tat. For more information on the different...
Hot Plasmids and Viral Preps - March 2021

Type

Blog Post

...repression, and editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence...
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

Type

Blog Post

...Assistant Professor of Sociology. University of Massachusetts Boston Christine Oslowski, PhD - Biotechnology...
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

Type

Blog Post

...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School....
xCas9: Engineering a CRISPR Variant with PAM Flexibility

Type

Blog Post

...variants with alternative PAMs like NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting...
Hot Plasmids and Viral Preps - May 2021

Type

Blog Post

...recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible CRISPRi library - Ampl133...
The AAVantages of AAV in CRISPR Screens

Type

Blog Post

...like split Cas9, or a smaller Cas9 ortholog like SaCas9. You could even venture away from the comfort of...
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Type

Blog Post

...scale right now is to grow up a bunch of cells, sacrifice them all, blow them up, and slam that onto a mass...
Fluorescent Protein Guide: Biosensors

Type

Collection

...PLoS One. 2014 Jun 5;9(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic...sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria. Biotechnol Biofuels...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...Global analysis of protein homomerization in Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org...
Getting to Know Expansion Microscopy

Type

Blog Post

...brief history and new avenues. Philosophical Transactions Royal Soc 380:20210110. https://doi.org/10.1098...
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Type

Blog Post

...Zhou Z, Smock DC, Tang K, Owens TK, Krishnappa N, Sachdeva R, Deutschbauer AM, Banfield JF, Doudna JA (2020...
Better Dyeing Through Chemistry & Small Molecule Fluorophores

Type

Blog Post

...classic rhodamines and their analogs without sacrificing their small size and membrane permeability (Figure...
Lentiviral Vector Uses and Overview

Type

Blog Post

...precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional activation from the 5’ ...
Tips for Screening with Yeast Two Hybrid Systems

Type

Blog Post

...Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively ...
Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

...that of the widely used SpCas9 and comparable to SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies...
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

Type

Blog Post

...So, think beyond Drosophila melanogaster or Saccharomyces cerevisiae (both fine research organisms) - ...
Supporting Reproducibility with a Connected ELN

Type

Blog Post

...electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged to be part of the vibrant...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Type

Blog Post

...Chicanos/Hispanics and Native Americans in Science’s (SACNAS) annual conference all foster mentorship in different...

Showing: 81 - 100 of 135 results

Results per page:

Plasmids 101: Cre-lox

Overcoming the Challenges of Lentiviral Production

Hot Plasmids and Viral Preps - March 2021

Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

xCas9: Engineering a CRISPR Variant with PAM Flexibility

Hot Plasmids and Viral Preps - May 2021

The AAVantages of AAV in CRISPR Screens

Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Fluorescent Protein Guide: Biosensors

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Getting to Know Expansion Microscopy

INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Better Dyeing Through Chemistry & Small Molecule Fluorophores

Lentiviral Vector Uses and Overview

Tips for Screening with Yeast Two Hybrid Systems

Cpf1: A New Tool for CRISPR Genome Editing

What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

Supporting Reproducibility with a Connected ELN

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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