We narrowed to 129 results for: sacs

1. Addgene: The First Twenty Years

   Type

   Blog Post

   ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...

2. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   Blog Post

   ...RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method...

3. The Future of Research Symposium Boston 2015

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   Blog Post

   ...University School of Medicine and the University of Massachusetts Medical School. Graduate education programs ...

4. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   Blog Post

   ...Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on...

5. Plasmids 101: Cre-lox

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   Blog Post

   ...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another...

6. Overcoming the Challenges of Lentiviral Production

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   Blog Post

   ...systems such that it no longer requires the viral transactivator, Tat. For more information on the different...

7. Hot Plasmids and Viral Preps - March 2021

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   Blog Post

   ...repression, and editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence...

8. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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   Blog Post

   ...Assistant Professor of Sociology. University of Massachusetts Boston Christine Oslowski, PhD - Biotechnology...

9. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ...variants with alternative PAMs like NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting...

10. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...like split Cas9, or a smaller Cas9 ortholog like SaCas9. You could even venture away from the comfort of...

11. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

    ...recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible CRISPRi library - Ampl133...

12. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    Blog Post

    ...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School....

13. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ...scale right now is to grow up a bunch of cells, sacrifice them all, blow them up, and slam that onto a mass...

14. Neurodegeneration Research Collection

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    Collection

    ...including: Anti-KChIP1 K+ channel [K55/7R] Anti-Fig4/Sac3 [N202/7R] Anti-Ankyrin-G [N106/36] Generate anti-kinesin...

15. Rinehart Lab Phosphoprotein Reagents

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    Collection

    ...al., 2011). Follow up work from the Rinehart and Issacs labs at Yale have made improvements to this system...

16. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ...Zhou Z, Smock DC, Tang K, Owens TK, Krishnappa N, Sachdeva R, Deutschbauer AM, Banfield JF, Doudna JA (2020...

17. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...Global analysis of protein homomerization in Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org...

18. Getting to Know Expansion Microscopy

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    Blog Post

    ...brief history and new avenues. Philosophical Transactions Royal Soc 380:20210110. https://doi.org/10.1098...

19. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post

    ...classic rhodamines and their analogs without sacrificing their small size and membrane permeability (Figure...

20. Lentiviral Vector Uses and Overview

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    Blog Post

    ...precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional activation from the 5’ ...