We narrowed to 120 results for: sag

1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   ...different plasmid, which may lead to unequal gene dosage and high variability of protein coexpression. By...

2. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ...Schwessinger, which resulted in greater access and usage of the materials. To provide greater visibility ...

3. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ...JChristopher Anderson. "Rapid optimization of gene dosage in E. coli using DIAL strains." Journal of biological...

4. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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   ...through our Twitter feeds and dealing with social messages and postings,” says Susan. There are also team...

5. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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   ... unionization at their institutions. One clear message that institutions have sent, with the lack of transparency...

6. Antibodies 101: Choosing the Right Isotype

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   ...; Cytochemistry (Vol. 46, Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813...

7. Open Resources and Plasmid Tools For Studying C. elegans

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   ...open science and share resources and tools. That message was fully exemplified in Cori Bargmann’s keynote...

8. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   ... Here! Munter, S.D., Görnemann, J., Derua, R., Lesage, B., Qian, J., Heroes, E., Waelkens, E., Eynde,...

9. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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   ...help you to become more assertive and resolve disagreements with your supervisor, even if he or she is a...

10. Of Myc and Men

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    ...al.’s results mean the end of 9E10’s time in the saga of the Myc tag? Hardly! 9E10’s accessibility and...

11. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...Self-renewal High Low once isolated from the human body Usage Universal. Can generate all adult cell types. Limited...

12. Plasmids 101: Degron Tags

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    ...for imaging. Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC) was developed...

13. A Guide to Starting Your Own Journal Club

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    ...casual request via email, in person, or instant messaging but we as scientists often prefer data. At Addgene...

14. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...biology and CRISPR genome engineering technologies. Message or follow him on twitter @Vopinator.     References...

15. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also theoretically...

16. The Importance of a Fun Workplace: Company Culture at Addgene

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    .... No, we’re not all BFFs. Yes, sometimes teams disagree. Yes, sometimes work is a bummer when you’d rather...

17. Negotiating Work and Life: How to Find the Joy

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    ...vacation alone with your partner – no kids. Get a massage – no guilt. Have recreational time with your friends...

18. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...off-target editing, and, for RNA editing, fine-tuning dosage so that enough wild-type MECP2 is made inside the...

19. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    ...to make a polygamous species monogamous. Anolis sagrei (Doug Menke) No methods were available to create...

20. Bioinformatics at Addgene

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    ...counting, or sequence assembly), but the order and usage of the scripts needs to be customized in the pipeline...