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Showing: 1 - 20 of 1010 results

  1. pSaGuide

    Plasmid
    #64710
    Purpose
    Cloning and expression of Staphylococcus aureus guide RNA
    Depositor
    Kiran Musunuru
    Insert
    Staphylococcus aureus guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Availability
    Academic Institutions and Nonprofits only
    64710-plasmid-map-sequence-id-108923

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  3. hprtSAGFP

    Plasmid
    #41594
    Purpose
    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences
    Depositor
    Maria Jasin
    Article
    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.
    Insert
    SA-GFP reporter
    Use
    Mouse Targeting; Dsb repair reporter substrate
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    41594-plasmid-map-sequence-id-228244

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  12. pTUBmCardinalSagCAT.dna

    Plasmid
    #166236
    Purpose
    mCardinal consituitive reporter, CAT marker
    Depositor
    David Sibley
    Insert
    mCardinal
    Use
    Tags
    Expression
    Mutation
    none
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    166236-plasmid-map-sequence-id-329836

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  13. pMAT_M13_6xSAG

    Plasmid
    #197096
    Purpose
    This plasmid contains the coding sequence for the control peptide M13, which contains a linker with six repeats of serine-alanine-glycine. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_C
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    Control peptide M13, which contains a linker with six repeats of serine-alanine-glycine (SAG)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    197096-plasmid-map-sequence-id-386824

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  14. AAVS1-SAGFP

    Plasmid
    #127100
    Purpose
    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    SA-GFP reporter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    127100-plasmid-map-sequence-id-247555

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  15. pEF 1-SAG12-4

    Plasmid
    #20092
    Depositor
    Antonio Richart
    Insert
    SAG12
    Use
    Synthetic Biology; Plant gateway vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    20092-plasmid-map-sequence-id-9464

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  17. pSAG1::CAS9-U6::sgUPRT

    Plasmid
    #54467
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.
    Depositor
    David Sibley
    Article
    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.
    Insert
    SAG1 5' UTR
    Use
    CRISPR; Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    54467-plasmid-map-sequence-id-84808

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Showing: 1 - 20 of 1010 results