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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 94 results for: sgrna

Showing: 81 - 94 of 94 results

  1. CRISPR Guide

    Type
    Guide
    ...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site by...efficiency PE3 - increased efficiency; an additional sgRNA directs Cas9n to a nearby site, driving mismatch...in nature and is also commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the ... D. E. (2014). Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation. Nature...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nature Biotechnology . 34 (...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids...plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional...

  7. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.” Genetics 200(2) (2015): 431-441. PubMed PMID:...Assembly. Using BsaI, gRNAs are cloned into one of 12 pYLsGRNA plasmids, which contain various promoters and ...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...

  11. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Figure 2): Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; ~8 kb packaging...

  12. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each essential gene. Koo...

  13. Lentiviral Vector Guide

    Type
    Guide
    ...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Walker Jackson 78624 sgRNA(MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP...His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia Gaorav...TARDBP-Exon1-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP...133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43 sgRNA2 TARDBP U6 ALS Shawn...TIA1 His, T7 T7 ALS Thomas Tuschl 106108 px458_2A_GFP_sgRNA_EIF2AK2 EIF2AK2 U6 VWM disease Thomas Tuschl ...Episodic ataxia Xiaolong Wang 159788 pGL3-U6-sgRNA_KCNA1-mcherry KCNA1 U6 Episodic ataxia Xiaolong Wang...Parkinson's, FTD Eugene Yeo 214814 U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2 ATM CAG Ataxia telangiectasia Yannick...
Showing: 81 - 94 of 94 results