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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 94 results for: sgrna

Showing: 81 - 94 of 94 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...The library was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses...that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides against...obstacle. With CRISPR-X, dCas9 is used to recruit an sgRNA with MS2 hairpin binding sites, which then recruit...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids...plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...

  8. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.” Genetics 200(2) (2015): 431-441. PubMed PMID:...Assembly. Using BsaI, gRNAs are cloned into one of 12 pYLsGRNA plasmids, which contain various promoters and ...

  10. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Figure 2): Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; ~8 kb packaging...

  12. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each essential gene. Koo...

  13. Lentiviral Vector Guide

    Type
    Guide
    ...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Walker Jackson 78624 sgRNA(MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP...His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia Gaorav...TARDBP-Exon1-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP...133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43 sgRNA2 TARDBP U6 ALS Shawn...TIA1 His, T7 T7 ALS Thomas Tuschl 106108 px458_2A_GFP_sgRNA_EIF2AK2 EIF2AK2 U6 VWM disease Thomas Tuschl ...Episodic ataxia Xiaolong Wang 159788 pGL3-U6-sgRNA_KCNA1-mcherry KCNA1 U6 Episodic ataxia Xiaolong Wang...Parkinson's, FTD Eugene Yeo 214814 U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2 ATM CAG Ataxia telangiectasia Yannick...
Showing: 81 - 94 of 94 results