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...cells, are already resistant to this antibiotic due to stable integration of resistance genes in their genomes...selection agent. Pros: Stable (spectinomycin). Cons: Cost (spectinomycin) or stability (streptomycin), doesn...damage. Choosing an antibiotic resistance Choosing the best antibiotic resistance ultimately comes down to ...antibiotic and happy selecting!   Antibiotic Resistance Stability Time Saving?  Works for multiple organisms... M., & Larminat, F. (2005). Resistance to the antibiotic Zeocin by stable expression of the Sh ble gene...Plasmids need antibiotic resistance genes so that you can specifically isolate bacteria containing your...interest. But does it matter which antibiotic resistance gene you select? In this blog, we’ll break down...

...to compare the photostability of different fluorescent proteins? Measuring photostability Since it is clear... to quantify the photostability of fluorescent proteins. To determine photostability, an experiment is...Measuring photostability at realistic powers will provide better insight into the photostability in the ...Measuring photostability in living cells provides a much more realistic view of photostability. Selecting...This avoids substantial changes in excitation power and will help to keep the photostability of the probes...this series, we will discuss how to select a photostable fluorescent protein. Photobleaching is the irreversible...desirable to have fluorescent proteins that are photostable. On top of photobleaching, fluorescent proteins...

...concept. It is imperative that we all understand what antibiotic resistance is and how it develops so that we...Microbiology Pages What is antibiotic resistance? Antibiotic resistance is defined as microbial survival despite...generally organisms with multi-drug resistance are resistant to three or more different classes, or types...Learn How Antibiotic Resistance Genes Are Used in the Lab How does antibiotic resistance develop? There are...in which bacteria can become resistant to antibacterial drugs. Resistance can be intrinsic, meaning a ...means the bacterium is not naturally resistant but has become resistant somehow overtime. One fantastic example... example of intrinsic resistance would be Mycoplasma species’ resistance to beta-lactam antibiotics, like...

...the resources. Instead of creating a start up budget, create a start up list.  In this post we’ll help you...most start-up P.I.’s. It doesn’t have to be complicated, or difficult to create this list. Start BEFORE... much do you need to start your lab”. You can respond by saying, “The exact start up costs may vary greatly...Consulting, Inc. “How much do you need [for your lab start up]?” A hiring chair or dean will often ask a faculty...leads to poor outcomes; not enough funds to get started, wasting funds on unnecessary materials, or even... you successfully negotiate a start up award by giving you step by step instructions on creating a budget... in regards to the budget. Creating a budget We start by using your research specific aims. This is actually...

...recombination between the two FRT sites, resulting in the stable integration of the gene of interest into the genome... repetitive recombination leading to genomic instability and cell death. It is important to incorporate...

...zebrafish, mouse, rat, and human. Developed by the Stanley Qi Lab . CCTop - CRISPR/Cas9 target online predictor...Link opens in a new window) A Google groups forum started by the Feng Zhang Lab . Protocols Lab(s) Description...

...chromatin and want to start sharing your plasmids through Addgene, don’t hesitate to start the process! Addgene...illustrations from Leah Bury of Microscopic Art. I started thinking about this problem when I recently attended...megabase away, or looking at the histone methylation status at a double-strand break that needs repairing. ... Pederson Phil Sharp Julie Ahringer Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard...H3.1 H3.3 HIST1H1E HIST1H2BB HIST2H2BE RBX1 STAG2 Xist Your favorite gene! SWI/SNF Genes:       ...

... and increase your lab sustainability. For my other posts on lab sustainability, check out my Recycling...the quick things above, try joining or starting a sustainability project. This could be: Setting up a ...This is the last post in a blog series on sustainable practices in the lab, where I’ll list some easy...unimportant. With so many big contributors to the sustainability problem, how can one little change make a difference...reusable water bottle instead of buying yourself a big stack of plastic cups. By initiating little changes in...lab Here are a few easy and fast things you can start doing right away to reduce your consumption in the...whole-cell extract from 2009. Join a newsletter to stay up-to-date on all the latest news and encourage ...

... Commercial DNA Ladder Penn State Ladder: Homemade Plasmid Prep Penn State Ladder: Commercial Plasmid ...undergraduate students led by Dr. Song Tan at Penn State developed the plasmids, pPSU1 and pPSU2. When restriction...$200-$500 for 1000 lanes worth of ladder. The Penn State ladders are significantly cheaper. When using a ...Your Agarose Gel Other Resources Read about Gel Stains on the BitesizeBio Blog ...

...to a few limiting factors.  To start, the average health science startup requires a great deal more capital... CEO of miLab, a Boston-based startup focused on empowering early-stage scientists. You can think of miLab...while becoming a health science entrepreneur at 22. Start with your network You've spent your life making ...the feedback we received from our network.  We started with the idea of a biotechnology investment fund...for such a fund is an impossibility at our early stage.  After each pitch we made, we considered the feedback...backgrounds (an essential component to any successful startup management team). Sreyan is a research associate... The world is against you.  As I mentioned, you start at a disadvantage in the health science space if...

...good ideas to a SynBio startup At some point, our ideas got pretty good. We started doing extracurricular...co-founders of Revolution Bioengineering.  Last year we started a company. Revolution Bioengineering is two responsible...working in a new technical discipline that nobody understands (synthetic biology), building a product no one...a plant that could sense and respond to novel substances. We both had an applied science bent, so the ...postdoc in order to rebuild my resume, so I had started to entertain other options. Keira had it a little...occasionally thinking about how we could bypass the establishment hiring system altogether. We had a lot of ideas...and dissected with equal enthusiasm. It may have started as idle conversation to pass the time in a job ...

...interview with Crystal Shih Podcast breakdown Intro Chapter 1: Who is Crystal Shih? Crystal's educational...Gray at Caltech, Crystal moved on to a Postdoc in the chemistry department at MIT. Crystal now works on protein...skills. Outro Many thanks to our guest, Crystal Shih. Crystal Shih leads project and scientific teams ...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland...therapeutics at Novartis - listen to learn more about Crystal’s exciting career path, to gain insight into industry...to succeed in your own career. One pro tip from Crystal - be agile and willing to move on to new projects...how delegating can empower people Chapter 3: How Crystal advanced at Novartis, and how to prepare yourself...

...three larval stages where larvae molt in order to continue growing in the next larval stage. After the ...remain stable as homozygous alleles, others cannot. Geneticists need a method for maintaining stable lines...three-part series. We’ll first discuss how to get started on fly work in this post. The second post will ...the bees when it comes to fruit flies Drosophila start life as fertilized eggs that develop into embryos...the third larval stage, Drosophila will pupate, which takes about one week from the initial deposition of... visible dark spot. Pupae begin eclosion at the start of the day and throughout daytime. They mostly stop...through their translucent bodies. This is the ideal stage to select for females because you can be certain...

...transgenes in Cre transgenic lines. As part of our standard viral production , these viral vectors undergo... plasmid with PCR, purity assessment by silver staining, in vitro and (when possible) in vivo testing,...

...AiE0410m Cre Oligodendrocytes Whole Brain * = Substantial non-specific labeling observed Publications Ben-Simon...Daigle, T. L., Dwivedi, D., Way, S. W., Oster, A., Stafford, D. A., Mich, J. K., Taormina, M. J., Martinez...

...have been developed, which differ in the number, stability, and brightness of the fluorescent proteins used... (Cai et al., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red)...truncated, non-fluorescent form of YFP as the default state of the transgene such that recombination is necessary...not undergo recombination can be detected by immunostaining of the truncated YFP. In Thy1-Brainbow mice...2011) labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage will express...injecting a CMV-Brainbow construct at the embryonic stage. This technique could be used to target Brainbow...labeled by injecting Brainbow at the single-cell stage. Zebrabow, or “zebrafish brainbow,” is a collection...

...In 2008 the Quake Lab at Stanford University became interested in exploring biological dark matter – ...Preparations ahead of time included contacting YNP staff to arrange permits to visit places not accessible...voltmeter on top of the battery box to monitor charge status and a disconnect switch between the battery bank...September we arrived in YNP just as the weather was starting to turn chilly at the 8,000 foot elevation. Due...environmental samples Since the high temperatures and unstable ground around these hot springs make them extremely...telescoping poles to collect water samples from a distance. At each collection site, we took GPS coordinates... collected small water and sediment samples, stabilizing them in ethanol and/or glycerol, since there ...

...Recombinase-based state machines (RSMs), which take on a particular DNA-sequence configuration (state) based on...effects on the state cannot be undone and also limit the set of possible downstream states that can be achieved...order of inputs results in only one final state, then the state of the RSM will tell you precisely what ...might one read the state of an RSM? The most straightforward way to read the state of an RSM is to sequence...is to interrogate state with qPCR. The re-arrangement of DNA segments in each state of a register enables...certain states of interest. For example, in Figure 2 below we present a two-input, five-state GRSM register...green fluorescent proteins in each state. The three methods of state interrogation mentioned above (Sanger...

...example, I am not that interested in meeting movie stars, but when I met the astronaut Jim Lovell I was speechless...work one floor above mine in the lab of Dr. Jack Szostak, who went on to win the Nobel Prize. Dr. Doudna...whiteboard. The final image is pictured here…she started from a blank white slate and developed this over...paper in Science.  Interestingly, this project started with their labs studying the mechanisms of RNAi...

...entrepreneur must be OK with the constant fear of failure at all stages in the startup". To gain insight into "...team was unable to secure enough money to sustain a startup, he went to work for Flagship Ventures – a... his energy and effort into making the startup self-sustainable. Within a year from this decision, Mike...Development & Operations. Starting a biotech company When and how does a startup become "successful"? First... budding startup, I asked Mike to outline 3 pieces that are essential to the biotech startup puzzle: ...Assembly Standards Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)    ...occasionally pondered translating my research into a startup company. However, like many like-minded people,...