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We narrowed to 1,046 results for: sta

Showing: 81 - 100 of 1046 results

  1. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ... Commercial DNA Ladder Penn State Ladder: Homemade Plasmid Prep Penn State Ladder: Commercial Plasmid ...undergraduate students led by Dr. Song Tan at Penn State developed the plasmids, pPSU1 and pPSU2. When restriction...$200-$500 for 1000 lanes worth of ladder. The Penn State ladders are significantly cheaper. When using a ...Your Agarose Gel Other Resources Read about Gel Stains on the BitesizeBio Blog ...

  2. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...to a few limiting factors.  To start, the average health science startup requires a great deal more capital... CEO of miLab, a Boston-based startup focused on empowering early-stage scientists. You can think of miLab...while becoming a health science entrepreneur at 22. Start with your network You've spent your life making ...the feedback we received from our network.  We started with the idea of a biotechnology investment fund...for such a fund is an impossibility at our early stage.  After each pitch we made, we considered the feedback...backgrounds (an essential component to any successful startup management team). Sreyan is a research associate... The world is against you.  As I mentioned, you start at a disadvantage in the health science space if...

  3. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...good ideas to a SynBio startup At some point, our ideas got pretty good. We started doing extracurricular...co-founders of Revolution Bioengineering.  Last year we started a company. Revolution Bioengineering is two responsible...working in a new technical discipline that nobody understands (synthetic biology), building a product no one...a plant that could sense and respond to novel substances. We both had an applied science bent, so the ...postdoc in order to rebuild my resume, so I had started to entertain other options. Keira had it a little...occasionally thinking about how we could bypass the establishment hiring system altogether. We had a lot of ideas...and dissected with equal enthusiasm. It may have started as idle conversation to pass the time in a job ...

  4. Plasmid Collections

    Type
    Collection
    ...plasmids and science information you need before starting your experiment. Genome Engineering Genome Engineering...Communities Find a scientific community that has established a common place for deposited plasmids. ASAP Discovery...

  5. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...expectations to the best of our knowledge from the start. However, we know there’s a chance that things may...general estimated turnaround time above. After starting viral production, if we determine that we will...

  6. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    ...interview with Crystal Shih Podcast breakdown Intro Chapter 1: Who is Crystal Shih? Crystal's educational...Gray at Caltech, Crystal moved on to a Postdoc in the chemistry department at MIT. Crystal now works on protein...skills. Outro Many thanks to our guest, Crystal Shih. Crystal Shih leads project and scientific teams ...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland...therapeutics at Novartis - listen to learn more about Crystal’s exciting career path, to gain insight into industry...to succeed in your own career. One pro tip from Crystal - be agile and willing to move on to new projects...how delegating can empower people Chapter 3: How Crystal advanced at Novartis, and how to prepare yourself...

  7. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...three larval stages where larvae molt in order to continue growing in the next larval stage. After the ...remain stable as homozygous alleles, others cannot. Geneticists need a method for maintaining stable lines...three-part series. We’ll first discuss how to get started on fly work in this post. The second post will ...the bees when it comes to fruit flies Drosophila start life as fertilized eggs that develop into embryos...the third larval stage, Drosophila will pupate, which takes about one week from the initial deposition of... visible dark spot. Pupae begin eclosion at the start of the day and throughout daytime. They mostly stop...through their translucent bodies. This is the ideal stage to select for females because you can be certain...

  8. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...H840A nickase. The fusion enzyme is capable of installing targeted insertions, deletions, and point mutations...protection factor La to PEmax to enhance pegRNA stability epegRNA — additional protection added to 3’ tail...

  9. Distribution to Industry

    Type
    Collection
    ...Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive...Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic multigene constructs...

  10. Promega Plasmid Collection

    Type
    Collection
    ...two formats: pretransformed in bacteria as an agar stab 20 µg purified DNA in Tris buffer (500 ng/µL ± 25...window) is a 19.1 kDa and exceptionally bright and stable luciferase that enables highly sensitive detection...

  11. Plant Plasmids and Resources

    Type
    Collection
    ...and responsible innovation for improvement of sustainable agriculture and conservation. Medicinal Plant...in a new window) - The Taproot is a podcast to understand how scientific publications are created. Papers...

  12. p53 Pathway

    Type
    Collection
    ...interaction with negative regulators, increase its stability and DNA binding activity, and allow it to bind...regulator; also known as MDM4 Noxa Phorbol-12-myristate-13-acetate-induced protein 1; also known as PMAIP1...

  13. TALEN Guide

    Type
    Collection
    ...Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide recognition ...some restrictions (for example, the target must start with a T), but they still have potential targetable...

  14. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...have been developed, which differ in the number, stability, and brightness of the fluorescent proteins used... (Cai et al., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red)...truncated, non-fluorescent form of YFP as the default state of the transgene such that recombination is necessary...not undergo recombination can be detected by immunostaining of the truncated YFP. In Thy1-Brainbow mice...2011) labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage will express...injecting a CMV-Brainbow construct at the embryonic stage. This technique could be used to target Brainbow...labeled by injecting Brainbow at the single-cell stage. Zebrabow, or “zebrafish brainbow,” is a collection...

  15. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...Recombinase-based state machines (RSMs), which take on a particular DNA-sequence configuration (state) based on...effects on the state cannot be undone and also limit the set of possible downstream states that can be achieved...order of inputs results in only one final state, then the state of the RSM will tell you precisely what ...might one read the state of an RSM? The most straightforward way to read the state of an RSM is to sequence...is to interrogate state with qPCR. The re-arrangement of DNA segments in each state of a register enables...certain states of interest. For example, in Figure 2 below we present a two-input, five-state GRSM register...green fluorescent proteins in each state. The three methods of state interrogation mentioned above (Sanger...

  16. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ...In 2008 the Quake Lab at Stanford University became interested in exploring biological dark matter – ...Preparations ahead of time included contacting YNP staff to arrange permits to visit places not accessible...voltmeter on top of the battery box to monitor charge status and a disconnect switch between the battery bank...September we arrived in YNP just as the weather was starting to turn chilly at the 8,000 foot elevation. Due...environmental samples Since the high temperatures and unstable ground around these hot springs make them extremely...telescoping poles to collect water samples from a distance. At each collection site, we took GPS coordinates... collected small water and sediment samples, stabilizing them in ethanol and/or glycerol, since there ...

  17. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...example, I am not that interested in meeting movie stars, but when I met the astronaut Jim Lovell I was speechless...work one floor above mine in the lab of Dr. Jack Szostak, who went on to win the Nobel Prize. Dr. Doudna...whiteboard. The final image is pictured here…she started from a blank white slate and developed this over...paper in Science.  Interestingly, this project started with their labs studying the mechanisms of RNAi...

  18. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...entrepreneur must be OK with the constant fear of failure at all stages in the startup". To gain insight into "...team was unable to secure enough money to sustain a startup, he went to work for Flagship Ventures – a... his energy and effort into making the startup self-sustainable. Within a year from this decision, Mike...Development & Operations. Starting a biotech company When and how does a startup become "successful"? First... budding startup, I asked Mike to outline 3 pieces that are essential to the biotech startup puzzle: ...Assembly Standards Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)    ...occasionally pondered translating my research into a startup company. However, like many like-minded people,...

  19. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...into two stages: Dedifferentiation - Conversion of adult somatic cells into the pluripotent state. Redifferentiation...differentiated somatic cell reverts back to a pluripotent state, which then proliferates and is redifferentiated...lineage, without dedifferentiating into a pluripotent stage. Cellular reprogramming The reversal of a differentiated...differentiated cell type to an undifferentiated state and then redifferentiation into the cell type of choice...differentiated cells of choice. The dedifferentiation stage involves overexpression of four reprogramming factors...differentiated somatic cell to revert back to a pluripotent stage (iPSC formation) [2, 3]. The iPSCs then proliferate...reprogrammed to iPSCs successfully [10-12]. The next stage of reprogramming consists of redifferentiation of...

  20. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...recombination between the two FRT sites, resulting in the stable integration of the gene of interest into the genome... repetitive recombination leading to genomic instability and cell death. It is important to incorporate...
Showing: 81 - 100 of 1046 results