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We narrowed to 362 results for: tac

Showing: 81 - 100 of 362 results

  1. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...for at least six months. Future directions and obstacles to clinical CRISPR editing Given the success of...diseases to be treated clinically with CRISPR. Many obstacles remain for CRISPR gene editing, but given the ...Mireault AA, McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal...

  3. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...covalently linked molecules (i.e. photoswitches) attached near the binding pocket of an engineered receptor...PORTLs use a similar concept, except the ligand is attached to a protein binding partner or nanobody that ...allowing the endogenous signaling pathway to remain intact and be studied without confounding issues associated...

  4. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...piggyBac™, the cargo is inserted into the genome fully intact, while, with a standard DNA plasmid transfection...with any integrated transgene). To overcome this obstacle, a positive selection cassette can be incorporated...use of negative selection largely overcomes this obstacle. Furthermore, we have found that transfecting ...

  5. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...wall. Here, I will cover some of the best ways to tackle mycoplasma contamination before they enter your...dry state for a longer time. When they come in contact with a nutrient source, they start proliferating...while working and discard them after use. Human contact can spread contamination when you are working without...

  8. Optogenetics Plasmid Collection

    Type
    Collection
    ...control neuronal activity, scientists generally attach a fluorescent protein to the opsin in order to ...

  9. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...the publication or plasmid information page, or contact the depositing PI for more information on the experimental...

  12. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...community of creative scientists delivered some spectacular photos once again this year! All winners receive...and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis (@AlexDainis) ...

  13. p53 Pathway

    Type
    Collection
    ...early ageing-associated phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann ...

  14. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...virion surface – making it critical for viral attachment and fusion. Once inside a host cell, new evidence... of neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking...

  15. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...updates regarding the order will be sent to the contact email addresses provided. How do I place an order...billing address for your organization. We will contact you directly if we require a copy of your PO. ...

  16. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...single consensus lentiviral expression vector and attached a DNA barcode to each to make the library usable...certain environments, for example. “We’ve been contacted by investigators all over the world who are interested...

  20. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...nothing if not solving your way through a series of obstacles in pursuit of achieving knowledge. Or, as we in...result of competing desires or (3) the presence of obstacles that need to be overcome. Conflict, and its inherent...
Showing: 81 - 100 of 362 results