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We narrowed to 361 results for: tac

Showing: 81 - 100 of 361 results
  1. Transferable Skills Guide: External Collaborations

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    Blog Post
    ...needed to find the right contacts at the companies and then convince the contacts to give me some time to...collaborators’ professional developments without directly contacting them. One great way to transition from an internal...
  2. Antibiotic Resistance: An Old Solution but a New Problem

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    Blog Post
    ...antibiotics, like penicillin. These specific drugs attack the cell wall of bacterial cells; however, Mycoplasma..., is fight smarter, not harder. We can utilize tactics like rotating antibiotic treatments, so that exposure... public health.     References 1. O'Neill, J. "Tackling drug-resistant infections globally: final report...
  3. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ...you to visualize a target of interest within an intact cell, tissue, or, in some cases, a whole organism...use of antibodies to visualize a target within an intact sample, a cell in the case of ICC or a tissue in...antibody targets the antigen of interest but is not attached to a fluorescent molecule, instead a secondary...
  4. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post
    ...for at least six months. Future directions and obstacles to clinical CRISPR editing Given the success of...diseases to be treated clinically with CRISPR. Many obstacles remain for CRISPR gene editing, but given the ...Mireault AA, McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal...
  5. Viral Vectors 101: Optogenetic Tools

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    Blog Post
    ...covalently linked molecules (i.e. photoswitches) attached near the binding pocket of an engineered receptor...PORTLs use a similar concept, except the ligand is attached to a protein binding partner or nanobody that ...allowing the endogenous signaling pathway to remain intact and be studied without confounding issues associated...
  6. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...piggyBac™, the cargo is inserted into the genome fully intact, while, with a standard DNA plasmid transfection...with any integrated transgene). To overcome this obstacle, a positive selection cassette can be incorporated...use of negative selection largely overcomes this obstacle. Furthermore, we have found that transfecting ...
  7. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post
    ...wall. Here, I will cover some of the best ways to tackle mycoplasma contamination before they enter your...dry state for a longer time. When they come in contact with a nutrient source, they start proliferating...while working and discard them after use. Human contact can spread contamination when you are working without...
  8. #Deckthelab Contest Winners 2017

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    Blog Post
    ...community of creative scientists delivered some spectacular photos once again this year! All winners receive...and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis (@AlexDainis) ...
  9. A Scientist's Guide to Ebola

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    Blog Post
    ...virion surface – making it critical for viral attachment and fusion. Once inside a host cell, new evidence... of neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking...
  10. How Do I Place an Addgene Order Using a PO Number?

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    Blog Post
    ...updates regarding the order will be sent to the contact email addresses provided. How do I place an order...billing address for your organization. We will contact you directly if we require a copy of your PO. ...
  11. Modulate the Activity of 17 Signaling Pathways with One Kit!

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    Blog Post
    ...single consensus lentiviral expression vector and attached a DNA barcode to each to make the library usable...certain environments, for example. “We’ve been contacted by investigators all over the world who are interested...
  12. Summer SciComm: Science Storytelling

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    Blog Post
    ...nothing if not solving your way through a series of obstacles in pursuit of achieving knowledge. Or, as we in...result of competing desires or (3) the presence of obstacles that need to be overcome. Conflict, and its inherent...
  13. Summer SciComm Series: Master of SciComm

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    Blog Post
    ...again write for fun eventually saw an English minor tacked onto my BSc. With this decision I had unwittingly... ours reflect a growing effort to address this obstacle in its many forms. A short stroll from my office...
  14. Plasmids 101: TOPO Cloning

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    Blog Post
    ...from a different DNA strand comes along, it can attack this covalent bond thus joining the two DNA strands...topoisomerase I from rebinding, which results in more intact molecules. (Note that the amount of salt you add...
Showing: 81 - 100 of 361 results