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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 130 results for: tac promoter

Showing: 81 - 100 of 130 results

  1. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression pattern...pattern and an auxin inducible degron (AID) sequence attached to your protein of interest. TIR1 interacts with...

  3. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...skills. This is an era of social media and self-promotion, and personality is playing an increasingly important...on fluorescent proteins and their applications, tackles much broader issues in this brief volume. He examines... Watsons.” In Part Four, “New Science,” Zimmer tackles more recent examples of scientific advances, and...

  4. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...transgenic genome will simply express from whatever promoters were included in the vector (or are nearby in ...after all, among the more virulent viruses (it attacks the immune system directly). It may seem odder ...6 x 106 virus-like particles that we come into contact with every day (Prussin et al., 2015), only a small...what these vector particles do once they come in contact with target cells (Fig. 3).     Figure ...

  5. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...walk into any supermarket now without seeing a promotion for antibiotic-free, farmed meats. We can focus...antibiotics, like penicillin. These specific drugs attack the cell wall of bacterial cells; however, Mycoplasma..., is fight smarter, not harder. We can utilize tactics like rotating antibiotic treatments, so that exposure... public health.     References 1. O'Neill, J. "Tackling drug-resistant infections globally: final report...

  6. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    .... Therefore, the same reaction which ligates a promoter into an empty backbone can be repeated to ligate...ligate your gene of interest after the promoter, and so on. The following table provides information for... Suffix Enzymes: SpeI , NotI , PstI Scar: TACTAGAG or TACTAG Features: 8 bp Scar; No In-Frame Fusions;...

  7. Optogenetics Plasmid Collection

    Type
    Collection
    ...ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging PI Return to top ...ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to top Content last reviewed: 25 September...control neuronal activity, scientists generally attach a fluorescent protein to the opsin in order to ...

  8. A Look at Addgene's QC Process

    Type
    Blog Post
    ...they ordered contains the correct inserts, tags, promoters, and other critical functional components. Fun...If the QC team identifies a QC issue, they will contact the depositing lab to review the discrepancies....

  9. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...incorporating it into a fourth plasmid. In addition, the promoter of the 5’ long terminal repeat (LTR) is mutated...supernatant is collected, filtered, and titered. A major obstacle in obtaining usable lentivirus is producing a ...

  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, ...reducing the time it takes to image through a large z-stack (8).  One limitation of LSFMs using single side ...illumination, especially with large tissue, is that any obstacles (e.g. air bubbles or high concentration of fluorophores...

  11. AAV Molecular Tools

    Type
    Collection
    ...Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator...pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of the tet-off... 2 Thomas Jessell , Eiman Azim 45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, ..., bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation. 1, 5 Nirao Shah ...

  12. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...complexed with a gRNA that directs them to the promoter of a gene of interest, they have been shown to...target site, Cas9’s DNA binding capability remains intact while its nucleolytic activity is eliminated (Fig...

  13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...continuous outflow of phage, increasing stringency and promoting survival of only the broadest-targeting variants...available near the locus you’d like to modify. To tackle the PAM problem, researchers have engineered alternative...

  14. PhD Applications After COVID

    Type
    Blog Post
    ...feasible. Through the virtual systems created and promoted during COVID-19, online meetings with current ...other. Before COVID-19, financial and logistical obstacles could make touring potential programs and universities...

  15. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ... difference between them lies in the choice of promoter for ωRNA (reRNA) expression (see Figure 1). Both...5: Colony Screening Use Primer 92F (5′-cattacgcaattggacgacaac-3′) and Oligo 2 (specific to your target.... If you have specific questions, feel free to contact the Molla Lab (X account: @Kutub_joy). Dr. Kutubuddin...

  16. Tips for arabidopsis transformation

    Type
    Blog Post
    ...are binary gateway vectors and can be used for promoter swapping and generating N and C terminally tagged...transfer DNA (tDNA) into the genomes of the plants it attacks Growing the transformed agrobacterium culture Dipping...

  18. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...encoded on the envelope plasmid which contains a promoter, the envelope gene, and a polyA tail.   ...protein’s role in infecting cell lines without using intact SARS-CoV-2 virus. Scientists have been using similar...

  20. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow...benefits may be changing. Please also feel free to contact us if you have information that you think would...
Showing: 81 - 100 of 130 results