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Showing: 81 - 100 of 152 results

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CRISPR 101: Non-Homologous End Joining

Type

Blog Post

...for Efficient Genome Editing in Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128/mbio.02364...
Giving gRNAs a Facelift - Synthetic and Beyond

Type

Blog Post

...We’ve all heard “Get that tube on ice!!” and “I hope it isn’t degraded” when scientists talk about their...
Summer SciComm Series: Cognitive Load

Type

Blog Post

...three year olds is unlikely to be able to follow a YouTube video on PCR, while a senior lab scientist is likely...
Addgene's Tips for Plasmid Quality Control

Type

Blog Post

...carefully track samples through our lab using barcoded tubes and plates. The critical pieces of information for...
Plasmids 101: Golden Gate Cloning

Type

Blog Post

...vector and entry vector(s) are placed in a single tube containing the Type IIS enzyme and ligase. Although...
High Complexity Golden Gate Assembly with NEB’s DAD

Type

Blog Post

...reaction, using four-base overhangs, in a single tube. Their assembly had a predicted fidelity of 71% ...
Tips for Technical Support Calls

Type

Blog Post

...information about a product is printed right on the tube itself! Is the answer to your question on your...
Open Resources and Plasmid Tools For Studying C. elegans

Type

Blog Post

..., this CRISPR plasmid can be created in a single tube. You can also find additional SapTrap plasmids used...
Viral Vectors 101: Virus Safety

Type

Blog Post

...biosafety cabinet. For ultracentrifugation, heat sealed tubes can be used for extra security. Finally, when spinning...
10 Basic tips for mammalian cell culture

Type

Blog Post

...rather decant the solutions into disposable sterile tubes first. Not only does this make things more manageable...
Plasmids 101: Protein Expression

Type

Blog Post

...such as maize, tobacco, rice, sugarcane, and even tubers of potatoes have been used for protein expression...
Fluorescent Tagging of Endogenous Genes with SapTrap

Type

Blog Post

...elegans. SapTrap offers scientists a convenient one tube assembly reaction to generate high-throughput targeting...
AAV Vector Quality Control: Going the Extra Mile with NGS

Type

Blog Post

...humans have been known to make mistakes. What if a tube is mislabeled or someone grabs the wrong plasmid...
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Type

Blog Post

... on the dorsal side of its thorax to look like stubble (sb). For a better reference of various fly phenotypes...
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Type

Blog Post

...reticulum, the normally spiderweb-like pattern of tubules can be grossly distorted into dense stacked membranes...
Experimenting with New Careers while in Grad School

Type

Blog Post

...BadAstronomer, Emily Graslie from The Brain Scoop YouTube channel @Ehmee, and David Shiffman @WhySharksMatter...
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Type

Blog Post

...tools and how they work, watch Endy's 10-minute YouTube primer on BIL gates.) Addgene: Why is it so important...
Tips for arabidopsis transformation

Type

Blog Post

... dunk your plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are both great options for...
Transferable Skills Guide: Creativity

Type

Blog Post

...is supply chain shortages. When flasks, conical tubes or pipette tips are backordered for months, we have...
Viral Vectors 101: Preparing Pooled Libraries

Type

Blog Post

...which contains a collection of plasmids in a single tube, or a pre-made viral prep library. Addgene’s pooled...

Showing: 81 - 100 of 152 results

Results per page:

CRISPR 101: Non-Homologous End Joining

Giving gRNAs a Facelift - Synthetic and Beyond

Summer SciComm Series: Cognitive Load

Addgene's Tips for Plasmid Quality Control

Plasmids 101: Golden Gate Cloning

High Complexity Golden Gate Assembly with NEB’s DAD

Tips for Technical Support Calls

Open Resources and Plasmid Tools For Studying C. elegans

Viral Vectors 101: Virus Safety

10 Basic tips for mammalian cell culture

Plasmids 101: Protein Expression

Fluorescent Tagging of Endogenous Genes with SapTrap

AAV Vector Quality Control: Going the Extra Mile with NGS

Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Experimenting with New Careers while in Grad School

Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Tips for arabidopsis transformation

Transferable Skills Guide: Creativity

Viral Vectors 101: Preparing Pooled Libraries

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