...pipette tip snowflakes, hang stockings filled with tube rack “gifts”, or prepare for the 2022 New Year. ...

...a 2D barcoded tube (see next section) and every time something is removed from this tube, the barcode ...contained within the tube, but more than that, they also help us keep track of the tubes’ physical location... 2D barcode and LIMS: Barcoded Tube Plate Freezer Rack Freezer Tubes are assigned a location within ...because every barcode on every tube contains information about that tube’s appropriate location. The barcode...each individual tube.  If a plate of samples is ever dropped, we can simply scan the tubes and know what..., we prepare three sets of 2D barcoded tubes. Two of the tubes are used for long term storage (one is ... the sample tubes that we use match those of the appropriate deposit. Using barcoded tubes is part of ...

... of tubes’ o-rings are, in fact, permeable to CO2 at low temperatures (-80°C) and once in the tube, the...What we did next: Venting the tubes We then tested whether venting the tubes prior to thaw released the ... and then vented the tubes by completely removing the caps, incubating the tubes in an upright position...and then recapping the tubes prior to thaw. We compared vented and non-vented tubes to controls that were...returning the tubes to the -80°C freezer after storage on dry ice allowed the CO2 to leave the tubes, and would...thawed the tubes after the indicated incubation periods, and measured the pH. We found that tubes subjected...therefore devised a few experiments to determine if our tubes were permeable to the CO2 released from dry ice,...

...recruited to microtubules under blue light, removing tubulin dimers from the microtubule body. B) Schematic...  Opto-katanin: a light-powered microtubule-cutting sword Microtubules are one of the most important components... several strategies are available to disrupt microtubules in cells, they have various disadvantages and...and limitations – for example, microtubule-destabilizing drugs are impossible to target to specific subcellular...published a new optogenetic tool for localized microtubule disassembly, Opto-katanin (Meiring et al., 2022...Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of Opto-katanin constructs...under CC-BY 4.0 license. A naturally occurring microtubule-severing protein, Katanin is of course named ...

... new tube. In the first example we’ve used inheritance to tightly couple the contents of a tube with ...with the tube itself. If something happens to the worm that requires a change of tube, there’s no clean way...second example we have a separate worm model and tube model, we can interchange them easily as needed....or a freezer. Some are disposable, like a plastic tube. Talk with your lab team about how they would like...

...vials with a specific amount of agar. There is a tube that goes from the needle to the flask with agar...dispense and you’re on your way. Label your tubes with a label maker. Label makers generate far more...your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is a simple table...roller to go over caps and push them into micronic tubes instead of pushing them in one by one. It seems ...instance, vial labeling would be much easier with a Tube Writer, which air brushes the information onto the...vials instead of requiring us to put a label on each tube by hand. We’ll be sure to tell you about more lab...

...Mycobacterium tuberculosis resistance to pyrazinamide (PZA) (a drug commonly used to treat tuberculosis) and in... drug resistance mechanisms of Mycobacterium tuberculosis Dr. Patricia Sheen, Universidad Peruana Cayetano...detection of PZA-resistance is necessary to improve tuberculosis care and control. In her current research, Sheen...improved therapies for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies biofilm formed...

...terms of total view count and videos published, YouTube reported a total of 865,995 views for the 926 video...  Check Out Addgene Videos Over at the Addgene Youtube Channel   After watching these trends for a few...these platforms were developing, Google acquired Youtube and has been growing its online video market ever... hosted on the platform, they can be hosted on YouTube, Vimeo, and other platforms (such as university...current-biology/abstract/S0960-9822(16)30873-9 Youtube: https://youtu.be/EHHhiawIdWM Saving salmon, one...

...add the various reagents to a single tube and simply flick the tube several times. This will help ensure...processes including automated vial filling, accurate tube-labeling, quick plate pouring, and much more. We...quick trick. Just flip, and press! Lab tip #2: Tube flick trick When combining reagents in the lab, ...

... Plasmids! Light controls the activity of a microtubule-associated protein to regulate cell growth dynamics...light by modifying EB1. EB1 normally sits on microtubules to attract other proteins involved in their ...into two halves (Fig. 1): one containing the microtubule binding domain and the second containing the ...modified EB1 protein are overexpressed in cells, microtubule growth can be switched off with blue light which...

... Limit tube transfer - the fewer opportunities your viral particles have to stick to your tubes, the higher...siliconized tubes to store samples as it is more difficult for viral particles to adhere to these tubes. Rinse...

...Streptococcus pneumoniae. Robert Husson lab's M. tuberculosis CRISPRi plasmids A group from the Husson lab... used CRISPRi to study essential genes in M. tuberculosis. dCas9 and a gRNA are expressed on pRH2502 and... (Singh et al. 2016). Sarah Fortune's lab M. tuberculosis CRISPRi plasmids Noting that the more common... for transcriptional silencing Mycobacterium tuberculosis and Mycobacterium smegmatis. In addition to ...Investigating essential gene function inMycobacterium tuberculosisusing an efficient CRISPR interference system. Nucleic...

...our guest blogger Kutubuddin Molla from the Pennsylvania State University Kutubuddin Molla is an Ag-biotechnologist...This post was contributed by Kutubuddin Molla, a Fulbright Visiting Scholar at the Pennsylvania State... Institute, Cuttack, Odisha, India. Currently Kutubuddin is a Fulbright Visiting Scholar at the Pennsylvania...

...way! Many thanks to guest blogger Kaustubh Kishor Jadhav! Kaustubh Kishor Jadhav, MSc is a Research Assistant...This post was contributed by guest blogger, Kaustubh Kishor Jadhav, a Research Assistant at MGMs Institute...lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain in the...

...used to convey the difference between a gradient tube before and after ultracentrifugation so that a scientist...each layer. The right panel illustrates a gradient tube before and after ultracentrifugation. The viral ...the bevel pointed up. A syringe at the top of the tube allows air to enter the column so the virus will...

...method procedure The procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant...are mixed with 3 ml of 2X DMEM in a 50 ml sterile tube (for 60 mm dishes only 1.5 ml of each are used)....agarose plug by rapid pipetting, as shown in the YouTube video. Before returning the plate to the incubator...

... K, et al (2015) Resolving bundled microtubules using anti-tubulin nanobodies. Nat Commun 6:7933. https... start re-conceptualizing actin filaments or microtubules. This issue is shared by other SRM techniques...

...same tube. You can clone up to 4 DNA fragments, in a specific order and orientation, in one tube, into...possible to set up the BP and LR reactions in the same tube, speeding up the cloning of the attB-PCR products...

...growing tips of microtubuli and plasmids tagged with MapTau to label entire microtubules. Find these fluorescent...best motif was from the Lck gene. For marking microtubules of mammalian cells, the author found that too...

...right, researchers of the world. Cap your Eppendorf tubes, hang up your pipettes, and seal up those plates...decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing routine...