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Showing: 1 - 20 of 151 results
  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...pmTurquoise2-Tubulin Microtubules alpha-tubulin mTurquoise2 Dorus Gadella 85045 pmScarlet_alphaTubulin_C1 Microtubules...alpha-tubulin mScarlet Dorus Gadella 85046 pmScarlet-H_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet-H...Dorus Gadella 85047 pmScarlet-i_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet-I Dorus Gadella 85054...EGFP Erich Nigg 158008 pCMV-mGold-Tubulin-C-18 Microtubules alpha-tubulin mGold Francois St-Pierre 158009...Francois St-Pierre 49149 mCh-alpha-tubulin Microtubules alpha-tubulin mCherry Gia Voeltz 89472 GFP-hChibby1...Michael Davidson 55148 mCherry-Tubulin-C-18 Microtubules alpha-tubulin mCherry* Michael Davidson 12298...12298 pIRESneo-EGFP-alpha Tubulin Microtubules alpha-tubulin EGFP Patricia Wadsworth 27123 eTC GFP beta-actin...
  2. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin Microtubules 87422 LMNB1-mEGFP AICSDP...Peroxisomes 101785 TUBA1B-mTagRFP-T AICSDP-28 mTagRFP-T Alpha-tubulin Microtubules 101786 ST6GAL1-mEGFP...
  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...182 Mycobacterium tuberculosis CRISPRi Library (RLC12) 163954 Inhibition M. tuberculosis Rock NA Varies ...Fly Yeast T. gondii E. coli S. pneumoniae M. tuberculosis M. smegmatis Green monkey ( C. sabaeus ) Kaposi's...
  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...trouble loading this video, please try viewing on YouTube . Abstract The prokaryotic clustered regularly ...filtering them through a 50 μm filter into a FACS tube. FACS sort the top ~3% of GFP positive cells in ...
  5. Antibody Guide

    Type
    Collection
    ...ubiquitously expressed protein such as GAPDH or tubulin (sometimes referred to as housekeeping genes). ...can also be sorted (separated and deposited into tubes or plate wells) by fluorescence activated cell sorting...
  6. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...a 2D barcoded tube (see next section) and every time something is removed from this tube, the barcode ...contained within the tube, but more than that, they also help us keep track of the tubes’ physical location... 2D barcode and LIMS: Barcoded Tube Plate Freezer Rack Freezer Tubes are assigned a location within ...because every barcode on every tube contains information about that tube’s appropriate location. The barcode...each individual tube.  If a plate of samples is ever dropped, we can simply scan the tubes and know what..., we prepare three sets of 2D barcoded tubes. Two of the tubes are used for long term storage (one is ... the sample tubes that we use match those of the appropriate deposit. Using barcoded tubes is part of ...
  7. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ... of tubes’ o-rings are, in fact, permeable to CO2 at low temperatures (-80°C) and once in the tube, the...What we did next: Venting the tubes We then tested whether venting the tubes prior to thaw released the ... and then vented the tubes by completely removing the caps, incubating the tubes in an upright position...and then recapping the tubes prior to thaw. We compared vented and non-vented tubes to controls that were...returning the tubes to the -80°C freezer after storage on dry ice allowed the CO2 to leave the tubes, and would...thawed the tubes after the indicated incubation periods, and measured the pH. We found that tubes subjected...therefore devised a few experiments to determine if our tubes were permeable to the CO2 released from dry ice,...
  8. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...recruited to microtubules under blue light, removing tubulin dimers from the microtubule body. B) Schematic...  Opto-katanin: a light-powered microtubule-cutting sword Microtubules are one of the most important components... several strategies are available to disrupt microtubules in cells, they have various disadvantages and...and limitations – for example, microtubule-destabilizing drugs are impossible to target to specific subcellular...published a new optogenetic tool for localized microtubule disassembly, Opto-katanin (Meiring et al., 2022...Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of Opto-katanin constructs...under CC-BY 4.0 license. A naturally occurring microtubule-severing protein, Katanin is of course named ...
  9. All Antibodies

    Type
    Collection
    ...Markers : Antibodies targeting popular markers like tubulin or epitope tags like Myc, GFP, and more. Neuroscience...
  10. Adenovirus Plasmids

    Type
    Collection
    ... 5 (HAdV5) genome that can be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder...
  11. mTOR Pathway

    Type
    Collection
    ...Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von Hippel-Lindau tumor suppressor...
  12. p53 Pathway

    Type
    Collection
    ...3 Siah Siah E3 ubiquitin protein ligase 1 TSC2 Tuberous sclerosis 2 TSP1 Thrombospondin 1 Return to top...
  13. Developing Lab Management Software for Biology

    Type
    Blog Post
    ... new tube. In the first example we’ve used inheritance to tightly couple the contents of a tube with ...with the tube itself. If something happens to the worm that requires a change of tube, there’s no clean way...second example we have a separate worm model and tube model, we can interchange them easily as needed....or a freezer. Some are disposable, like a plastic tube. Talk with your lab team about how they would like...
  14. Lab Automation at Addgene

    Type
    Blog Post
    ...vials with a specific amount of agar. There is a tube that goes from the needle to the flask with agar...dispense and you’re on your way. Label your tubes with a label maker. Label makers generate far more...your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is a simple table...roller to go over caps and push them into micronic tubes instead of pushing them in one by one. It seems ...instance, vial labeling would be much easier with a Tube Writer, which air brushes the information onto the...vials instead of requiring us to put a label on each tube by hand. We’ll be sure to tell you about more lab...
  15. Viral Production

    Type
    Collection
    ...matching system that follows the sample from the DNA tube all the way to the cleared viral preparation pool...
  16. Ras Pathway

    Type
    Collection
    ...metastasis TP53 Tumor protein p53 TSC TSC1 TSC2 Tuberous sclerosis VAV1 Vav guanine nucleotide exchange...
  17. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...Mycobacterium tuberculosis resistance to pyrazinamide (PZA) (a drug commonly used to treat tuberculosis) and in... drug resistance mechanisms of Mycobacterium tuberculosis Dr. Patricia Sheen, Universidad Peruana Cayetano...detection of PZA-resistance is necessary to improve tuberculosis care and control. In her current research, Sheen...improved therapies for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies biofilm formed...
  18. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...terms of total view count and videos published, YouTube reported a total of 865,995 views for the 926 video...  Check Out Addgene Videos Over at the Addgene Youtube Channel   After watching these trends for a few...these platforms were developing, Google acquired Youtube and has been growing its online video market ever... hosted on the platform, they can be hosted on YouTube, Vimeo, and other platforms (such as university...current-biology/abstract/S0960-9822(16)30873-9 Youtube: https://youtu.be/EHHhiawIdWM Saving salmon, one...
  19. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...add the various reagents to a single tube and simply flick the tube several times. This will help ensure...processes including automated vial filling, accurate tube-labeling, quick plate pouring, and much more. We...quick trick. Just flip, and press! Lab tip #2: Tube flick trick When combining reagents in the lab, ...
Showing: 1 - 20 of 151 results