... of tubes’ o-rings are, in fact, permeable to CO2 at low temperatures (-80°C) and once in the tube, the...What we did next: Venting the tubes
We then tested whether venting the tubes prior to thaw released the ... and then vented the tubes by completely removing the caps, incubating the tubes in an upright position...and then recapping the tubes prior to thaw. We compared vented and non-vented tubes to controls that were...returning the tubes to the -80°C freezer after storage on dry ice allowed the CO2 to leave the tubes, and would...thawed the tubes after the indicated incubation periods, and measured the pH.
We found that tubes subjected...therefore devised a few experiments to determine if our tubes were permeable to the CO2 released from dry ice,...