Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 961 results for: ARC

Showing: 491 - 500 of 961 results

  1. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...the College of Life Sciences that same year. Her research is focused on the escape mechanism of genome editing...Molecular Plant Sciences since December 2020. His research focuses on the efficiency of transposon-encoded...professor in Synthetic Biology in 2006. The Yang lab's research is focused on developing microbial genome editing...Sciences in September 2019 as a Ph.D. candidate. Her research focuses mainly on the development and application...

  2. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...users, we just released a major redesign of how search works on our site. For Addgenies, development includes...with our software. When this happens, we need to research and hopefully find a comparable library if it’...our users focus on the fun stuff, accelerating research and discovery. We work with transparency, gladly...the Addgene blog Learn about what went into our search engine update Find all Addgene news Resources ...

  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...adaptation for use in mammalian cells in 2012-2013. Researchers are now using this system in ever more creative...Hopefully this post will help get your in vivo research up and running as soon as possible! Selecting ...it’s time to use them in mouse embryos. When researchers first started using CRISPR/Cas9 for genome editing...RNA-guided endonucleases and nickases." Genome research 24.1 (2014): 132-141. PubMed PMID: 24253446. PubMed...

  4. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... variety of ways to manage my time. As a busy researcher, you’ve probably already started coming up with...hope you’ll find this post useful. After a bit of research and thinking about my own processes, I’ve broken...

  5. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul ...site-specific knock-in tool that can be harnessed by researchers for many applications. Your favorite Cas enzyme...generally low; to increase the frequency of edits, researchers have designed self-cleaving plasmids that liberate...repair pathway choice. Shrivastav M, et al Cell Research (2008). PubMed. Mechanisms of DNA double-strand...

  6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...variety of organisms.  While much of current CRISPR research focuses on its potential applications for human...engineering tools that are available to academic researchers through Addgene. CRISPR components for plant ...promoter and gRNA scaffold sequence and allow researchers to insert targeting oligos with minimal cloning... hand at CRISPR. References Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V (2013) Plant genome editing...

  7. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...products that resulted from their research, “aha” moments, and future research directions. Don’t worry if your...this file over the years and a talk about your research or work will write itself when the time comes....ask about how many people use plasmids in their research and then see how many of them have requested from...

  8. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...blue light-activated cation channel, from the archaebacteria Chlamydomonas reinhardtii. When exposed to ...combination with retinal) variations designed to give researchers flexibility in using optogenetic tools in their...concentration gradient, and of activation inhibitory (ArCH) proton pump passing ions against the concentration...proteins activate their signaling pathways allowing researchers greater control over when and where to activate...

  9. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...increasing affordability and accessibility, more researchers have been utilizing full plasmid sequencing services...sequencing that Addgene uses.) However, when some researchers received the sequencing results for their plasmids...numbers (Williams, Carnes, and Hodgson, 2009). More research is ongoing to determine plasmid features that ...monomeric plasmid compared to DH5α. However, more research is needed to determine which recA- strains are...

  10. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ... design of these constructs, allowing novice researchers to quickly and easily start genome editing (Schwartz...donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289...CRISPR-targeted homologous recombination. Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805...Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013) Heritable Custom Genomic...
Showing: 491 - 500 of 961 results