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We narrowed to 955 results for: ARC;

Showing: 491 - 500 of 955 results

  1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...in neuronal circuits or synaptic remodeling, researchers combine the Cre and FLP recombinases to control...combination of Cre, FLP, and inducible systems gives a researcher exquisite control over expression of multiple...Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question of how...-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org Find Cre-Lox Plasmids...

  2. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...adaptation for use in mammalian cells in 2012-2013. Researchers are now using this system in ever more creative...Hopefully this post will help get your in vivo research up and running as soon as possible! Selecting ...it’s time to use them in mouse embryos. When researchers first started using CRISPR/Cas9 for genome editing...RNA-guided endonucleases and nickases." Genome research 24.1 (2014): 132-141. PubMed PMID: 24253446. PubMed...

  3. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... variety of ways to manage my time. As a busy researcher, you’ve probably already started coming up with...hope you’ll find this post useful. After a bit of research and thinking about my own processes, I’ve broken...

  4. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul ...site-specific knock-in tool that can be harnessed by researchers for many applications. Your favorite Cas enzyme...generally low; to increase the frequency of edits, researchers have designed self-cleaving plasmids that liberate...repair pathway choice. Shrivastav M, et al Cell Research (2008). PubMed. Mechanisms of DNA double-strand...

  5. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...variety of organisms.  While much of current CRISPR research focuses on its potential applications for human...engineering tools that are available to academic researchers through Addgene. CRISPR components for plant ...promoter and gRNA scaffold sequence and allow researchers to insert targeting oligos with minimal cloning... hand at CRISPR. References Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V (2013) Plant genome editing...

  6. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...products that resulted from their research, “aha” moments, and future research directions. Don’t worry if your...this file over the years and a talk about your research or work will write itself when the time comes....ask about how many people use plasmids in their research and then see how many of them have requested from...

  7. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...blue light-activated cation channel, from the archaebacteria Chlamydomonas reinhardtii. When exposed to ...combination with retinal) variations designed to give researchers flexibility in using optogenetic tools in their...concentration gradient, and of activation inhibitory (ArCH) proton pump passing ions against the concentration...proteins activate their signaling pathways allowing researchers greater control over when and where to activate...

  8. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...increasing affordability and accessibility, more researchers have been utilizing full plasmid sequencing services...sequencing that Addgene uses.) However, when some researchers received the sequencing results for their plasmids...numbers (Williams, Carnes, and Hodgson, 2009). More research is ongoing to determine plasmid features that ...monomeric plasmid compared to DH5α. However, more research is needed to determine which recA- strains are...

  9. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...effectiveness and interpretation of RNAi. Fortunately, researchers studying plants and other organisms lacking this...concatenated. hpRNA has been extensively used in plant research and many different constructs are available to...and is a popular RNAi trigger in the mammalian research community. Fortunately, many tools for shRNA in...fluorescence readout of RNAi activity. As a plant researcher, the traditional hpRNA and visual screening method...

  10. Finding Your Science Policy Path

    Type
    Blog Post
    ...how and where you pursue it. It can manifest via research and data gathering, regulatory efforts, policy...fellowships have a strong focus on early career researchers who have decided to transition out of academia... host “Hill Days” where they train and bring researchers to Congress to advocate for increased science...incentivizing the real-world impacts of health services research. She is particularly interested in driving scientific...
Showing: 491 - 500 of 955 results