We narrowed to 653 results for: EPO
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TypeBlog Post...your western blot several times — some researchers report up to ten cycles! Just remember that a bit of sample...
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Plasmids 101: Modular Cloning
TypeBlog Post...As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog Post... to start is the 24-piece lac operon assembly deposited by NEB – and don’t forget to ask DAD for some ... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...sigh. The problem is that many of these studies report correlations without clearly testing whether a ... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...selection of guideRNAs. Of particular note, ABE8s reported in this work did not cause genome-wide, guide-... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...ways how scientists have used Addgene’s plasmid repository and viral service for their behavioral studies... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...vectors used to create knockout mice include a reporter gene, which replaces the targeted gene and provides... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post... and the type of virus, there have been varied reports of titer losses from 5% to 50% per freeze thaw ... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...characterized a set of prokaryotic terminators and deposited several with Addgene (1). Prokaryotic polyadenylation...