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We narrowed to 938 results for: Kin

Showing: 491 - 500 of 938 results

  1. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...scenarios below for a CRISPR experiment which involves making specific knock-out edits in a gene(s) of interest...off-target frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes and...certain Cas enzymes are less compatible with the bulkiness that accompanies mispairing of bases. To reduce...enzyme swaps will only reduce off targets where nicking/cleaving of DNA occurs, not for deadCas activator...

  2. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... channel with fast kinetics may be required, whereas a channel with slower kinetics can be used for lower...each light source used.   Channel and activation kinetics Some channels turn on and off very rapidly in ...thorough characterization data, like spectral and kinetic activation information, in the supplemental data...

  3. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...counting the number of puncta in each cell. When making figures for a paper or presentation, make sure ...binding non-specifically – try optimizing your blocking and washing conditions or consider using a different...concentrations of all the different antibodies, blocking, washing, and sample preparation steps, in order...Immunofluorescence Staining. In W. H. Yong (Ed.), Biobanking (Vol. 1897, pp. 299–311). Springer New York. ...

  6. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ... single dependent variable. I know what you’re thinking – this really sounds like a two-way ANOVA. And...calculated p values from smallest to largest and ranking them (1 being the smallest value). It calculates... you want to know type of distribution you are working with, graphing data with a histogram is sometimes... friendly neighborhood biostatistician happy by making your statistical analysis plan when you design ...

  7. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...stability. Optimizing antibodies for better pharmacokinetics and reduced immunogenicity allows for more...the specific mutations you choose, whether it's tweaking the antibody-antigen interface or fine-tuning ... mutants for the specific functionality you’re looking for. For example, if you were aiming to increase...-antibody interactions in a plate-based format, making it suitable for comparing relative affinities across...

  8. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ...eBook collection is a handy resource for anyone looking to use antibody-based applications in their research...: What is an Antibody” quite useful. If you’re looking to design your first flow cytometry experiment,...the Antibody 101 eBook is a great place to start looking for answers.    We hope you find the latest addition...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring kinase activity (Ni, Titov, & Zhang, 2006), quantifying...pressure-test your FRET assay to make sure it is working? This article addresses these questions, which ...s best to consider these cases separately when talking about the tools that are available. Let’s consider...Denis V. Titov, and Jin Zhang. "Analyzing protein kinase dynamics in living cells with FRET reporters." ...

  10. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...Fly-related Issues in the Lab A significant drawback of working with fruit flies is their ability to escape their...experienced the minor yet consistent annoyance of walking into a fly lab and finding ourselves accosted by...system in a video filmed at Addgene:     If you're working in fly research, I hope you find this new technology...
Showing: 491 - 500 of 938 results